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Your search keyword '"Santos, Filipe"' showing total 6 results
Start Over Author "Santos, Filipe" Journal acs synthetic biology
6 results on '"Santos, Filipe"'

1. Interlaboratory Reproducibility in Growth and Reporter Expression in the Cyanobacterium Synechocystis sp. PCC 6803

Author

Mager, Maurice, primary, Pineda Hernandez, Hugo, additional, Brandenburg, Fabian, additional, López-Maury, Luis, additional, McCormick, Alistair J., additional, Nürnberg, Dennis J., additional, Orthwein, Tim, additional, Russo, David A., additional, Victoria, Angelo Joshua, additional, Wang, Xiaoran, additional, Zedler, Julie A. Z., additional, Branco dos Santos, Filipe, additional, and Schmelling, Nicolas M., additional

Published
2023
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6. Nonhierarchical Flux Regulation Exposes the Fitness Burden Associated with Lactate Production in Synechocystis sp. PCC6803.

Author

Du W, Angermayr SA, Jongbloets JA, Molenaar D, Bachmann H, Hellingwerf KJ, and Branco Dos Santos F

Subjects
Fructosediphosphates metabolism, L-Lactate Dehydrogenase metabolism, Mannitol analogs & derivatives, Mannitol metabolism, Allosteric Regulation genetics, Lactic Acid metabolism, Synechocystis metabolism
Abstract

Cyanobacteria are mostly engineered to be sustainable cell-factories by genetic manipulations alone. Here, by modulating the concentration of allosteric effectors, we focus on increasing product formation without further burdening the cells with increased expression of enzymes. Resorting to a novel 96-well microplate cultivation system for cyanobacteria, and using lactate-producing strains of Synechocystis PCC6803 expressing different l-lactate dehydrogenases (LDH), we titrated the effect of 2,5-anhydro-mannitol supplementation. The latter acts in cells as a nonmetabolizable analogue of fructose 1,6-bisphosphate, a known allosteric regulator of one of the tested LDHs. In this strain (SAA023), we achieved over 2-fold increase of lactate productivity. Furthermore, we observed that as carbon is increasingly deviated during growth toward product formation, there is an increased fixation rate in the population of spontaneous mutants harboring an impaired production pathway. This is a challenge in the development of green cell factories, which may be countered by the incorporation in biotechnological processes of strategies such as the one pioneered here.

Published
2017
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