1. Single-Fluorophore Indicator to Explore Cellular and Sub-cellular Lactate Dynamics
- Author
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Camila Aburto, Alex Galaz, Angelo Bernier, Pamela Yohana Sandoval, Sebastián Holtheuer-Gallardo, Iván Ruminot, Ignacio Soto-Ojeda, Helen Hertenstein, Johanna Aurelia Schweizer, Stefanie Schirmeier, Tammy Paulina Pástor, Gonzalo Antonio Mardones, Luis Felipe Barros, and Alejandro San Martín
- Subjects
Fluid Flow and Transfer Processes ,Drosophila melanogaster ,Ionophores ,Process Chemistry and Technology ,Fluorescence Resonance Energy Transfer ,Animals ,Bioengineering ,Lactic Acid ,Endoplasmic Reticulum ,Instrumentation ,Fluorescent Dyes - Abstract
Lactate is an energy substrate and an intercellular signal, which can be monitored in intact cells with the genetically encoded FRET indicator Laconic. However, the structural complexity, need for sophisticated equipment, and relatively small fluorescent change limit the use of FRET indicators for subcellular targeting and development of high-throughput screening methodologies. Using the bacterial periplasmic binding protein TTHA0766 from
- Published
- 2022