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The anomalously high recovery of solution gas drive in some heavy oil reservoirs has been associated with foamy oil. The effects of external factors such as temperature, permeability, and the pressure depletion rate on foamy oil flow have been studied sufficiently, but few studies are available on the effect of heavy oil itself. In order to investigate the effect of oil viscosity and the solution gas-oil ratio on foamy oil, 11 tests of solution gas drive through a sandpack were carried out in this work. The results show that a typical foamy oil solution gas drive exists in three stages, which are the oil phase expansion stage, the foamy oil flow stage, and the oil-gas two-phase flow stage. As the oil viscosity decreases, the foamy oil flow stage shortens, resulting in reduced recovery of this stage significantly. In the experiment with an oil viscosity of 200 mPa·s, foamy oil flow was not observed. A lower limit of oil viscosity should exist for steady flow of foamy oil, which is considered to be approximately 600 mPa·s according to the experimental results. As the solution gas-oil ratio increases, the oil recovery first increases and then decreases. Foamy oil flow could be observed clearly when the solution gas-oil ratio was between 10 and 26 Sm

In this work, a biological engineering-based biosilica-yeast hybrid material was developed. It was obtained by the aggregation of Yarrowia lipolytica through biosilicification catalyzed using genetically displayed silicatein on its cell surface. With orthosilicate or seawater as the substrate, the silicatein-displayed yeast could aggregate into flocs with a flocculation efficiency of nearly 100%. The resulting floc was found to be a sheetlike biosilica-yeast hybrid material formed by the biosilica-mediated immobilization of yeast cells via cross-linking and embedding, turning the original hydrophilicity of yeast cells into hydrophobicity. In addition, this material was characterized to be porous with an average pore diameter of approximately 10 μm and porosity of over 70%. Because of these properties, this hybrid material could achieve enhanced removal efficiencies for chromium ions and n-hexadecane, which were both above 99%, as compared to the free cells of Y. lipolytica in aqueous environments. Importantly, this hybrid material could be recultivated to generate new batches of yeast cells that maintain parallel properties to the first generation so that the same hybrid material could be reproduced with unchanged highly efficient removal of chromium and n-hexadecane to those of the first generation, demonstrating that this biosilica-yeast hybrid material was living and renewable. This work presented a novel way of harnessing silicatein and Y. lipolytica to achieve biological synthesis of a living inorganic-organic hybrid material that has potential to be applied in water treatment.