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The sequencing of the genomes of the B05.10 and T4 strains of the fungus Botrytis cinerearevealed an abundance of novel biosynthetic gene clusters, the majority of which were unexpected on the basis of the previous analyses of the fermentation of these and closely related species. By systematic alteration of easy accessible cultivation parameters, using chemical induction with copper sulfate, we have found a cryptic sesquiterpenoid family with new structures related to eremophil-9-ene, which had the basic structure of the sesquiterpene (+)-5-epiaristolochene ((+)-4-epieremophil-9-ene). An expression study of the sesquiterpene cyclase genes present in the Botrytis cinereagenome, under culture conditions, is reported. In general, a 3 day delay and a higher BcSTCgenes expression were observed when copper (5 ppm) was fed to the fermentation broth. In addition, to the observed effect on the BcBOT2(BcSTC1) gene, involved in the biosynthesis of the botrydial toxin, a higher expression level for BcSTC3and BcSTC4was observed with respect to the control in the strain B05.10. Interestingly, under copper conditions, the BcSTC4gene was the most expressed gene in the Botrytis cinereaUCA992 strain. In vitroevaluation of the biological role of these metabolites indicates that they contributed to the conidial development in B. cinereaand appear to be involved in self-regulation of the production of asexual spores. Furthermore, they promoted the formation of complex appressoria or infection cushions.