1. Redox Responsive Polymersomes for Enhanced Doxorubicin Delivery
- Author
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Aradhana Nayal, Veena Koul, and Chetan Nehate
- Subjects
biology ,Biocompatibility ,Chemistry ,media_common.quotation_subject ,0206 medical engineering ,Biomedical Engineering ,02 engineering and technology ,021001 nanoscience & nanotechnology ,biology.organism_classification ,020601 biomedical engineering ,Biomaterials ,HeLa ,chemistry.chemical_compound ,In vivo ,Polymersome ,Polycaprolactone ,medicine ,Biophysics ,Doxorubicin ,0210 nano-technology ,Internalization ,Cytotoxicity ,media_common ,medicine.drug - Abstract
In the present investigation, the potential of a novel, self-assembled, biocompatible, and redox-sensitive copolymer system with disulfide bond was explored for doxorubicin (DOX) delivery through polymersome nanostructures of ∼120 nm. The polymer system was synthesized with less steps, providing a high yield of 86%. The developed polymersomes showed admirable biocompatibility with high dose tolerability in vitro and in vivo. The colloidal stability of DOX-loaded polymersomes depicted a stable and uniform particle size over a period of 72 h. The cellular internalization of polymersomes was assessed in HeLa and MDA-MB-231 cell lines, where enhanced cellular internalization was observed. The dose-dependent cytotoxicity was observed for DOX-loaded polymersomes by MTT cytotoxicity assay in the above cell lines. The tumor suppression studies were assessed in Ehrlich ascites tumor (EAT) carrying Swiss albino mice, where polymersomes exhibited a 7.16-fold reduction in tumor volume correlated with control and 5.39...
- Published
- 2021