1. Harnessing Polyhydroxyalkanoates and Pressurized Gyration for Hard and Soft Tissue Engineering
- Author
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Barbara Lukasiewicz, Eleanor J. Humphrey, Rinat Nigmatullin, Ipsita Roy, Phoebe L. Heseltine, Mohan Edirisinghe, Jonathan I. Dawson, Pooja Basnett, Richard O.C. Oreffo, Caroline S. Taylor, Qasim A. Majid, Mehrie Behbehani, John W. Haycock, Janos M. Kanczler, Upulitha Eranka Illangakoon, Cesare M. Terracciano, Sian E. Harding, and Rupy Kaur Matharu
- Subjects
Materials science ,Rotation ,Cell Survival ,Cells ,Induced Pluripotent Stem Cells ,02 engineering and technology ,03 medical and health sciences ,Mice ,Tissue engineering ,Cell Line, Tumor ,Elastic Modulus ,Ganglia, Spinal ,Pressure ,Animals ,Humans ,General Materials Science ,Myocytes, Cardiac ,Induced pluripotent stem cell ,030304 developmental biology ,0303 health sciences ,Tissue Engineering ,Tissue Scaffolds ,Regeneration (biology) ,Polyhydroxyalkanoates ,Biomaterial ,Soft tissue ,Cell Differentiation ,Mesenchymal Stem Cells ,021001 nanoscience & nanotechnology ,Cell biology ,Rats ,Transplantation ,Chorioallantoic membrane ,Schwann Cells ,0210 nano-technology ,Chickens ,Porosity ,Ex vivo - Abstract
Organ dysfunction is a major cause of morbidity and mortality. Transplantation is typically the only definitive cure, challenged by the lack of sufficient donor organs. Tissue engineering encompasses the development of biomaterial scaffolds to support cell attachment, proliferation, and differentiation, leading to tissue regeneration. For efficient clinical translation, the forming technology utilized must be suitable for mass production. Herein, uniaxial polyhydroxyalkanoate scaffolds manufactured by pressurized gyration, a hybrid scalable spinning technique, are successfully used in bone, nerve, and cardiovascular applications. Chorioallantoic membrane and in vivo studies provided evidence of vascularization, collagen deposition, and cellular invasion for bone tissue engineering. Highly efficient axonal outgrowth was observed in dorsal root ganglion-based 3D ex vivo models. Human induced pluripotent stem cell derived cardiomyocytes exhibited a mature cardiomyocyte phenotype with optimal calcium handling. This study confirms that engineered polyhydroxyalkanoate-based gyrospun fibers provide an exciting and unique toolbox for the development of scalable scaffolds for both hard and soft tissue regeneration.
- Published
- 2021