1. Electrochemical Quantification of Glycated and Non-glycated Human Serum Albumin in Synthetic Urine
- Author
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Attar, Aisha M, Richardson, Mark B, Speciale, Gaetano, Majumdar, Sudipta, Dyer, Rebekah P, Sanders, Emily C, Penner, Reginald M, and Weiss, Gregory A
- Subjects
Analytical Chemistry ,Engineering ,Chemical Sciences ,Diabetes ,Biosensing Techniques ,Electrochemical Techniques ,Enzymes ,Immobilized ,Equipment Design ,Glycation End Products ,Advanced ,Humans ,Models ,Biological ,Serum Albumin ,Serum Albumin ,Human ,Tetrahydrofolate Dehydrogenase ,Glycated Serum Albumin ,human serum albumin ,glycated albumin ,polythiophene ,iminodiacetic acid ,boronic acid ,dihydrofolate reductase ,biosensor ,Nanoscience & Nanotechnology ,Chemical sciences ,Physical sciences - Abstract
A polymer-based electrode capable of specific detection of human serum albumin, and its glycated derivatives, is described. The sensor is constructed from a glass microscope slide coated with a synthesized, polythiophene film bearing a protected, iminodiacetic acid motif. The electrode surface is then further elaborated to a functional biosensor through deprotection of the iminodiacetic acid, followed by metal-affinity immobilization of a specific and high-affinity, albumin ligand. Albumin was then quantified in buffer and synthetic urine via electrochemical impedance spectroscopy. Glycated albumin was next bound to a boronic acid-modified, single-cysteine dihydrofolate reductase variant to quantify glycation ratios by square-wave voltammetry. The platform offers high sensitivity, specificity, and reproducibility in an inexpensive arrangement. The detection limits exceed the requirements for intermediate-term glycemic control monitoring in diabetes patients at 5 and 1 nM for albumin and its glycated forms, respectively.
- Published
- 2019