1. Morphological, Stability, and Hypoglycemic Effects of New Gliclazide-Bile Acid Microcapsules for Type 1 Diabetes Treatment: the Microencapsulation of Anti-diabetics Using a Microcapsule-Stabilizing Bile Acid
- Author
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Sangeetha Mathavan, Frank Arfuso, Hani Al-Salami, and Nigel Chen-Tan
- Subjects
Drug ,Male ,medicine.drug_class ,Cell Survival ,media_common.quotation_subject ,Drug Compounding ,Pharmaceutical Science ,Administration, Oral ,Capsules ,02 engineering and technology ,Aquatic Science ,Pharmacology ,030226 pharmacology & pharmacy ,Cell Line ,Diabetes Mellitus, Experimental ,Bile Acids and Salts ,03 medical and health sciences ,Mice ,Random Allocation ,0302 clinical medicine ,Drug Stability ,Oral administration ,Diabetes mellitus ,Drug Discovery ,medicine ,Myocyte ,Animals ,Hypoglycemic Agents ,Gliclazide ,Viability assay ,Rats, Wistar ,Ecology, Evolution, Behavior and Systematics ,media_common ,Ecology ,Bile acid ,Chemistry ,General Medicine ,021001 nanoscience & nanotechnology ,medicine.disease ,Ursodeoxycholic acid ,Rats ,Diabetes Mellitus, Type 1 ,Treatment Outcome ,0210 nano-technology ,Agronomy and Crop Science ,medicine.drug - Abstract
When we administered orally a mixture of the anti-diabetic drug, gliclazide (G) and a primary bile acid, they exerted a hypoglycemic effect in a rat model of type 1 diabetes (T1D), but stability of mixture was limited. We aimed to develop and characterize microcapsules incorporating G with a microcapsule-stabilizing bile acid, ursodeoxycholic acid (UDCA). Sodium alginate (SA)-based microcapsules were prepared with either G or G with UDCA and analyzed in terms of morphological, physico-chemical, and electro-chemical characteristics at different pH and temperatures. The microcapsules' effects on viability on muscle cell line (C2C12) and on diabetic rats' blood glucose levels and inflammatory profiles were also examined. Bile acid-based microcapsules maintained their morphology, showed good stability, and compatibility profiles, and the incorporation of UDCA resulted in less G content per microcapsule (p
- Published
- 2018