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Plastic waste is a global environmental burden. Polyurethanes (PU), toxic and ubiquitous synthetic polymers, do not biodegrade quickly, leading to their rapid accumulation in the soil and water environments. Highly efficient PU-degrading microorganisms are rare in nature and are of fundamental importance for achieving circular plastic economy. Bacterial isolates from groundwater, originating from magmatogenic massif and Tertiary basin within metamorphic area, as well as soil isolates collected from various pristine (PS) and contaminated sites (CS), were screened using PU model compound Impranil® DLN-SD (IMP) as sole C source to identify PU-degrading isolates. Phylogenetic analysis of 16S rRNA gene sequences from IMP-degrading isolates was performed using the neighbor-joining method to observe their clustering. Thirty one of 96 isolates (32.3 %) from groundwater and 18 of 220 isolates (8.2%) from soil produced prominent IMP-clearing zones. Thirteen IMPdegrading isolates from each type of environment, belonging to 8 genera (Pseudomonas, Proteus, Enterobacter, Flavobacterium, Serratia, Pantoea, Acinetobacter and Stenotrophomonas) for groundwater and to 6 genera (Streptomyces, Pseudomonas, Rhodococcus, Achromobacter, Bacillus and Paenibacillus) for soil environment, were included in phylogenetic analysis. No clear grouping of groundwater and soil isolates was observed, indicating that isolates are too distinct. Stronger clustering was observed for groundwater compared to soil isolates. For groundwater, strongest clustering was observed for 2 isolates belonging to Proteus genus, 2 belonging to Flavobacterium and 2 to Pseudomonas. For soil samples, strongest clustering was observed for 3 isolates belonging to genus Streptomyces. There was no clear grouping within isolates from CS and PS. In the future, wider range of environmental niches should be included in screening efforts for development of biocatalytic processes for management of plastic waste. Subterranean ecosyste