Jamyuang, Chanettee, Phoonlapdacha, Phanphen, Chongviriyaphan, Nalinee, Chanput, Wasaporn, Nitisinprasert, Sunee, and Nakphaichit, Massalin
Several studies have reported a complex microbial community in human breast milk. This community impacts the shape of the infant gut microbiota and consequently impacts host health. Lactobacillusis an important probiotic and has many applications in the functional food industry. This study isolated and evaluated the potential probiotic bacteria from human milk. Two Lactobacillusspecies, L. plantarumand L. pentosus, were isolated from the breast milk of Thai women. L. pentosusHM04-22, L. pentosusHM04-3, L. plantarumHM04-80, L. plantarumHM04-88 and L. plantarumHM01-1 showed good adhesion activity (> 55%) and resistance in gastric (pH 2) and bile (pH 8) conditions. Characterization of the probiotic properties indicated that all selected Lactobacillusisolates had anti-adhesion properties against Escherichia coliand SalmonellaTyphimurium. Lactobacillusisolates protected Caco-2 cells from pathogen adhesion at 25–40%. In addition, the five selected strains presented anti-inflammatory properties by reducing interleukin (IL)-8 expression at 0.14 ± 0.16 to 0.52 ± 0.117-fold. However, the strains had no effect on the expression of tight junction genes, including zona occludens (ZO)-1, occludin and claudin-1. In conclusion, five selected Lactobacillusisolates from human milk were candidates for use as probiotics to promote health. However, more tests in animal models and clinical trials need to be performed.