1. On proper simulation of phenomena influencing image formation in fluorescence microscopy
- Author
-
Stanislav Stejskal, Martin Maška, Ludek Matyska, Vladimír Ulman, Jan Bella, and David Svoboda
- Subjects
Image formation ,0303 health sciences ,Ground truth ,Computer science ,business.industry ,Real image ,Photobleaching ,030218 nuclear medicine & medical imaging ,Image (mathematics) ,03 medical and health sciences ,0302 clinical medicine ,Medical imaging ,Fluorescence microscope ,Computer vision ,Artificial intelligence ,business ,Focus (optics) ,030304 developmental biology - Abstract
The simulation plays an important role in biomedical image analysis as it can inherently provide large collections of image data with absolute ground truth. Compared to real image data typically annotated by some expert, the computer generated data still lacks the authenticity due to the simplifications of various natural phenomena. In this paper, we focus on simulating the photobleaching effect and the uneven illumination, being simplified or even omitted from the majority of present simulation toolkits, to considerably improve the authenticity of computer generated data.
- Published
- 2014
- Full Text
- View/download PDF