1. Microelectrothermofluidic packaging for single chip integrated viral RNA extraction and RT-PCR microdevices
- Author
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Hong Miao Ji, Tae Goo Kang, Yu Chen, Ming Yi Daniel Ang, Li Zhang, Xiaowu Zhang, Guang Kai Ignatius Tay, Kum Cheong Tang, Siow Pin Melvin Tan, and Siti Rafeah Mohamed Rafei
- Subjects
Microheater ,Reverse transcription polymerase chain reaction ,Materials science ,Microchannel ,business.industry ,Microfluidics ,Optoelectronics ,Fluidics ,Nanotechnology ,Integrated circuit packaging ,Solid phase extraction ,business ,Microfabrication - Abstract
This paper presents a microelectrothermofluidic packaging of integrated microdevice for viral ribonucleic acid (RNA) extraction from blood samples and its amplification through a reverse transcription (RT)-polymerase chain reaction (PCR) method for application to the point-of-care (POC) infectious disease diagnostics. Integrated microdevice consists of solid phase extraction microchannel structure, microfluidic mixer, and meander-shaped microchamber for RT-PCR. Metallic microheater and resistive temperature detection (RTD) microsenosr is monolithically integrated with fluidic components. In order to secure both electrical and fluidic interconnections, spring-loaded electrical pin and drilled microchannel structure has been design in polycarbonate micropackaging structure. In the biotesting, we have demonstrated to detect dengue serotype III virus from the 80µl of blood sample with 80pfu virus concentration.
- Published
- 2010