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Glycyrrhic acid (GA) and liquiritin are the two classes of bioactive components in licorice with known pharmacological effects. In our study, different methods were compared to separate GA an-d liquiritin. Solid-phase extraction (SPE) method was verified to separate GA and liquiritin effectively. The method is fast, easy, reliable, and inexpensive; especially in the quality control. Moreover, we built a new method to determine glycyrrhizic acid and liquiritin simultaneously using reversed phase high performance liquid chromatography (RP-HPLC) In the HPLC analyze, gradient elution and two wavelength were used . The regression line for GA was y =15340.6x+12.81, the regression for liquiritin line was y =9269.1x+20.63, The recovery rates were 101.3% and 99.4%; RSD were 1.01% and 0.92%.

Forsythia suspense is a widely used Chinese traditional medicine. Forsythoside B and phillyrin are the major bioactive components in Forsythia suspense. In this study, macroporous resin method was used to separate forsythoside B and phillyrin. It indicated that compared with solvent extraction and absorption by alumina and other common separation method, macroporous resin method can separate forsythoside B and phillyrin effectively. Moreover, this study built a new method to determine forsythoside B and phillyrin simultaneously relied on Reversed phase high performance liquid chromatography (RP-HPLC). The linearity studies, Stability, Precision, and reproducibility test satisfy the determination request.