1. Tuning the Hydrophilic–Hydrophobic Balance of Molecular Polymer Bottlebrushes Enhances their Tumor Homing Properties
- Author
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Ramamurthi, Parathan, Zhao, Zhongchao, Burke, Eamonn, Steinmetz, Nicole F, and Müllner, Markus
- Subjects
Cancer ,Rare Diseases ,Biotechnology ,Bioengineering ,Ovarian Cancer ,Nanotechnology ,Development of treatments and therapeutic interventions ,5.1 Pharmaceuticals ,Animals ,Drug Delivery Systems ,Hydrophobic and Hydrophilic Interactions ,Mice ,Nanoparticles ,Neoplasms ,Polymers ,3D cell culture ,biodistribution ,cellular interactions ,polymer bottlebrushes ,polymer nanoparticles ,tumor mouse model ,Medicinal and Biomolecular Chemistry ,Biomedical Engineering ,Medical Biotechnology - Abstract
Nanoparticle (NP)-based drug delivery systems are promising in anticancer therapy, capable of delivering cargo with superior selectivity and achieving enhanced tumor accumulation compared to small-molecule therapeutics. As more efforts are being devoted to NP development, molecular polymer bottlebrushes (MPBs) have gained attention as a potential drug delivery vehicle. To date, the influence of various MPB parameters such as size, shape, and surface charge in determining tumor penetrability have been systematically probed. However, the role of amphiphilicity, specifically the hydrophilic-hydrophobic balance, remains unexplored. In this study, a series of MPBs are employed with varied hydrophobicity levels to reveal a dependence between MPB composition, cell association, and tumor homing. The data indicates that increasing levels of hydrophobicity in MPBs (to a certain level) demonstrate only marginal effects in vitro but reveals enhanced tumor homing in a mouse model of ovarian cancer in vivo, where more hydrophilic MPBs exhibit low tissue deposition and low tumor homing. In contrast, more hydrophobic MPBs show significant tumor accumulation and homing due to their engineered hydrophobicity.
- Published
- 2022