1. Generation of an induced pluripotent stem cell line (UBCi001-A) from a presymptomatic individual carrying the R418X progranulin gene mutation
- Author
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Frew, J, Wu, X, Hsiung, GY, Feldman, HH, Mackenzie, IR, and Nygaard, HB
- Subjects
Medical Biotechnology ,Biomedical and Clinical Sciences ,Stem Cell Research - Nonembryonic - Human ,Stem Cell Research ,Regenerative Medicine ,Stem Cell Research - Induced Pluripotent Stem Cell - Non-Human ,Clinical Research ,Genetics ,Stem Cell Research - Induced Pluripotent Stem Cell ,Stem Cell Research - Nonembryonic - Non-Human ,Stem Cell Research - Induced Pluripotent Stem Cell - Human ,Cell Differentiation ,Cells ,Cultured ,Codon ,Nonsense ,Female ,Fibroblasts ,Frontotemporal Lobar Degeneration ,Heterozygote ,Humans ,Induced Pluripotent Stem Cells ,Progranulins ,Biological Sciences ,Medical and Health Sciences ,Developmental Biology ,Medical biotechnology ,Oncology and carcinogenesis - Abstract
Induced pluripotent stem cells (iPSCs) were generated from peripheral blood-derived erythroid progenitor cells obtained from a presymptomatic female carrying the heterozygous R418X progranulin (GRN) nonsense mutation, known to cause autosomal dominant frontotemporal lobar degeneration. Erythroid progenitor cells were reprogrammed into iPSCs using integration free episomal plasmids which enables exogenous expression of the Yamanaka factors. The pluripotent potential of the iPSCs was validated through expression of pluripotency factors and their capacity to differentiate into the three primary germ layers. The cells were confirmed to carry the described mutation and shown to have a normal karyotype.
- Published
- 2019