1. Protein disulfide isomerase–endoplasmic reticulum resident protein 57 regulates allergen-induced airways inflammation, fibrosis, and hyperresponsiveness
- Author
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Hoffman, Sidra M, Chapman, David G, Lahue, Karolyn G, Cahoon, Jonathon M, Rattu, Gurkiranjit K, Daphtary, Nirav, Aliyeva, Minara, Fortner, Karen A, Erzurum, Serpil C, Comhair, Suzy AA, Woodruff, Prescott G, Bhakta, Nirav, Dixon, Anne E, Irvin, Charles G, Janssen-Heininger, Yvonne MW, Poynter, Matthew E, and Anathy, Vikas
- Subjects
Biomedical and Clinical Sciences ,Immunology ,Clinical Research ,Health Effects of Indoor Air Pollution ,Lung ,Asthma ,2.1 Biological and endogenous factors ,Aetiology ,Inflammatory and immune system ,Respiratory ,Allergens ,Animals ,B-Lymphocytes ,Biopsy ,Caspase 3 ,Cytokines ,Disease Models ,Animal ,Female ,Fibrosis ,Gene Expression ,Humans ,Male ,Mice ,Mice ,Transgenic ,Protein Disulfide-Isomerases ,Respiratory Hypersensitivity ,Respiratory Mucosa ,T-Lymphocytes ,bcl-2 Homologous Antagonist-Killer Protein ,Unfolded protein response ,endoplasmic reticulum stress ,asthma ,house dust mite ,protein disulfide isomerase ,endoplasmic reticulum protein 57 ,recombination-activating gene 1 ,epithelium ,airways hyperresponsiveness ,Allergy - Abstract
BackgroundEvidence for association between asthma and the unfolded protein response is emerging. Endoplasmic reticulum resident protein 57 (ERp57) is an endoplasmic reticulum-localized redox chaperone involved in folding and secretion of glycoproteins. We have previously demonstrated that ERp57 is upregulated in allergen-challenged human and murine lung epithelial cells. However, the role of ERp57 in asthma pathophysiology is unknown.ObjectivesHere we sought to examine the contribution of airway epithelium-specific ERp57 in the pathogenesis of allergic asthma.MethodsWe examined the expression of ERp57 in human asthmatic airway epithelium and used murine models of allergic asthma to evaluate the relevance of epithelium-specific ERp57.ResultsLung biopsy specimens from asthmatic and nonasthmatic patients revealed a predominant increase in ERp57 levels in epithelium of asthmatic patients. Deletion of ERp57 resulted in a significant decrease in inflammatory cell counts and airways resistance in a murine model of allergic asthma. Furthermore, we observed that disulfide bridges in eotaxin, epidermal growth factor, and periostin were also decreased in the lungs of house dust mite-challenged ERp57-deleted mice. Fibrotic markers, such as collagen and α smooth muscle actin, were also significantly decreased in the lungs of ERp57-deleted mice. Furthermore, adaptive immune responses were dispensable for house dust mite-induced endoplasmic reticulum stress and airways fibrosis.ConclusionsHere we show that ERp57 levels are increased in the airway epithelium of asthmatic patients and in mice with allergic airways disease. The ERp57 level increase is associated with redox modification of proinflammatory, apoptotic, and fibrotic mediators and contributes to airways hyperresponsiveness. The strategies to inhibit ERp57 specifically within the airways epithelium might provide an opportunity to alleviate the allergic asthma phenotype.
- Published
- 2016