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This book reviews the latest developments and applications in the field of biosensing, providing readers with an update of the earlier, successful edition, Biosensing for the 21st Century. In this book, readers will find comprehensive key information on the advances and challenges in biorecognition elements and transduction principles, including examples of new materials and new methods. Ranging from engineering chimeric enzymes to the progress in electrochemical sensing of drugs, the book discusses the latest innovations in the field and thus, provides the reader with a profound overview. In the field of biological recognition the focus will be on switchable sensors by engineering new proteins, the development of Molecular Imprinted Polymers (MIPs) for proteins or even larger biological entities, the construction of complex nucleic acid – protein structures for affinity sensing and the application of whole microbes. The book covers new trends in signal transduction which includes graphene-based field effect transistors, nanopores designed for high selectivity and sensitivity in analysis, plasmonic and nanophotonic sensing applying metal nanostructures up to new developments in microarray construction. An introduction in to the challenges in skin-interfaced systems for continuous biochemical sensing, offers a glimpse into the future of wearable technologies. Furthermore, actual areas of application of new sensing strategies such as the detection of various types of viruses and bacteria or the analysis of drugs will be highlighted. Each chapter provides a scholarly yet accessible perspective on the latest trends in biosensor technology. Given its breadth, the book has an interdisciplinary appeal and engages a wide readership, from students and researchers to practitioners in academia and industry, interested in the fields of biochemical/biomedical engineering and in biosensing methodologies and applications.

This book chronicles the role of advanced nanomaterials and surface engineering technologies in the development of point-of-care biosensors for health and environmental monitoring. All aspects of nanomaterial synthesis and characterization, functionalization methods, sensing surface engineering, signal amplification strategies, use of innovative technologies to enhance sensor efficiency and performances, and innovative applications of nanobiosensors to tackle real-life problems are discussed in this book with a focus on optical and electrochemical based sensing. It also covers the detection of infectious diseases and various disease biomarkers, smartphone-based biosensing, and portable diagnostics module developments with a discussion on the working mechanisms of these devices in various domains. The book also illustrates the recent trends in biosensing, and an overview of the challenges and probable solutions for the translation of biosensors from laboratory prototypes to commercial success. ​

This book presents select proceedings of the North East Research Conclave (NERC 2022). The book mainly covers research related to healthcare and related technologies. It includes broad bioengineering applications divided into four parts, namely, biomaterials and tissue engineering, biomedical science and engineering, drug delivery and therapeutics and cell and molecular biology. The book encompasses the latest research and advancements in the field of healthcare and related technologies. This book also describes a few studies based on computer simulations in bioengineering. In addition, the fundamental and basic aspects are also discussed for the better understanding of readers. The book can be a valuable reference for beginners, researchers and professionals interested in the field of healthcare and related technologies.

New Experimental Probes for Enzyme Specificity and Mechanism, Volume 685, the latest release in the Methods of Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics including Subverting Hedgehog Protein Autoprocessing by Chemical Induction of Paracatalysis, New Mechanistic Probes to Identify Novel Substrates for N-Myristoyltransferases, Phosphonate and a Fluorophosphonate Analogues of D Glucose 6 Phosphate as Active-Site Probes of 1L-Myo-Inositol 1 phosphate Synthase, Kinetic Mechanism of Nicotine-Degrading Enzyme Probed by Stopped-Flow Kinetic Analyses, Kinetics and Mechanism for Enzyme-Catalyzed Reactions of Substrate Pieces, and more. Additional chapters cover Kinetics and Mechanism for Reactions of Enzyme Pieces, Evaluation of allostery for the bienzyme assembly of a 3-deoxy-D-arabino heptulosonate-7-phosphate synthase and chorismate mutase, Recognition and Catalysis of Reactions of Chiral Substrates by Mandelate Racemase, Innovative and emerging modalities of EGFR kinase inhibitors, Characterization of the Aminoacrylate Intermediate of Tyrosine Phenol-Lyases, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology serials - Updated release includes the latest information on New Experimental Probes for Enzyme Specificity and Mechanism

This book gives a comprehensive overview of recent advancements in both theory and practical implementation of plasmonic probes. Encompassing multiple disciplines, the field of plasmonics provides a versatile and flexible platform for nanoscale sensing and imaging. Despite being a relatively young field, plasmonic probes have come a long way, with applications in chemical, biological, civil, and architectural fields as well as enabling many analytical schemes such as immunoassay, biomarkers, environmental indexing, and water quality sensing, to name but a few. The objective of the book is to present in-depth analysis of the theory and applications of novel probes based on plasmonics, with a broad selection of specially-invited chapters on the development, fabrication, functionalization, and implementation of plasmonic probes as well as their integration with current technologies and future outlook. This book is designed to cater to the needs of novice, seasoned researchers and practitioners in academia and industry, as well as medical and environmental fields.

This book presents tools and techniques for the development of miniature biosensors and their applications. The initial chapters discuss the advancements in the development of the transduction techniques, including optical, electrochemical, and piezoelectric, which are used for miniaturized biosensors. The book also reviews several technologies, such as nanotechnology, nanobiotechnology, immune-technology, DNA-technology, micro-manufacturing technology, electronic-circuit technology to increase the miniaturization and sensitivity of the biosensor platform. Subsequently, the chapters illustrate the applications of miniaturized biosensing systems in point-of-care monitoring of treatment and disease progression, environmental monitoring, food control, drug discovery, forensics, and biomedical research. Towards the end, the book discusses the advanced applications of biosensors in water quality monitoring, especially on-line detection systems and on-site detection of pesticides, heavy metals and bacteria in water. This book is an invaluable source for scientists working in biochemical engineering, bioengineering, and biomedical engineering in academia and industry.

This book presents a collection of chapters on modern bioelectrochemistry focusing on new materials for biodevice, bioelectrosynthesis and bioenergy. The chapters cover protein engineering, semiconductors, biorecognition, graphene-based bioelectronics, bioelectrosynthesis, biofuel cells, bioinspired batteries and biophotovoltaics.

The detection and measurement of the dynamic regulation and interactions of cells and proteins within the living cell are critical to the understanding of cellular biology and pathophysiology. The multidisciplinary field of molecular imaging of living subjects continues to expand with dramatic advances in chemistry, molecular biology, therapeutics, engineering, medical physics and biomedical applications. Molecular Imaging: Principles and Practice, Volumes 1 and 2, Second Edition provides the first point of entry for physicians, scientists, and practitioners. This authoritative reference book provides a comprehensible overview along with in-depth presentation of molecular imaging concepts, technologies and applications making it the foremost source for both established and new investigators, collaborators, students and anyone interested in this exciting and important field. - The most authoritative and comprehensive resource available in the molecular-imaging field, written by over 170 of the leading scientists from around the world who have evaluated and summarized the most important methods, principles, technologies and data - Concepts illustrated with over 600 color figures and molecular-imaging examples - Chapters/topics include, artificial intelligence and machine learning, use of online social media, virtual and augmented reality, optogenetics, FDA regulatory process of imaging agents and devices, emerging instrumentation, MR elastography, MR fingerprinting, operational radiation safety, multiscale imaging and uses in drug development - This edition is packed with innovative science, including theranostics, light sheet fluorescence microscopy, (LSFM), mass spectrometry imaging, combining in vitro and in vivo diagnostics, Raman imaging, along with molecular and functional imaging applications - Valuable applications of molecular imaging in pediatrics, oncology, autoimmune, cardiovascular and CNS diseases are also presented - This resource helps integrate diverse multidisciplinary concepts associated with molecular imaging to provide readers with an improved understanding of current and future applications

This book includes selected papers from the 4th International Conference on Computational Vision and Bio Inspired Computing (ICCVBIC 2020), held in Coimbatore, India, from November 19 to 20, 2020. This proceedings book presents state-of-the-art research innovations in computational vision and bio-inspired techniques. The book reveals the theoretical and practical aspects of bio-inspired computing techniques, like machine learning, sensor-based models, evolutionary optimization and big data modeling and management that make use of effectual computing processes in the bio-inspired systems. As such it contributes to the novel research that focuses on developing bio-inspired computing solutions for various domains, such as human–computer interaction, image processing, sensor-based single processing, recommender systems and facial recognition, which play an indispensable part in smart agriculture, smart city, biomedical and business intelligence applications.

This book covers the discovery of molecular biomarkers, the development of laboratory testing techniques and their clinical applications, focusing on basic research to clinical practice. It introduces new and crucial knowledge and ethics of clinical molecular diagnosis. This book emphasizes the applications of clinical molecular diagnostic test on health management, especially from different diseased organs. It lets readers to understand and realize precision healthcare.

Numerous genetic methods can be utilised to link a phenotype to a single molecular target but annotated small molecule chemical probes and even entire chemogenomic libraries are increasingly being used as a complementary approach. This book will comprehensively cover the state of the art in chemical probes and best practice for use in target discovery, illustrated throughout with examples. Ideal for students and established biochemists, the book will also cover new technologies for probe discovery, new probe modalities, the new field of probes for RNA targets and the mature field of kinase chemical probes.

The area of molecular imaging has matured over the past decade and is still growing rapidly. Many concepts developed for molecular biology and cellular imaging have been successfully translated to in vivo imaging of intact organisms. Molecular imaging enables the study of processes at a molecular level in their full biological context. Due to the high specificity of the molecular readouts the approach bears a high potential for diagnostics. It is fair to say that molecular imaging has become an indispensable tool for biomedical research and drug discovery and development today.This volume familiarizes the reader with the concepts of imaging and molecular imaging in particular. Basic principles of imaging technologies, reporter moieties for the various imaging modalities, and the design of targeted probes are described in the first part. The second part illustrates how these tools can be used to visualize relevant molecular events in the living organism. Topics covered include the studies of the biodistribution of reporter probes and drugs, visualization of the expression of biomolecules such as receptors and enzymes, and how imaging can be used for analyzing consequences of the interaction of a ligand or a drug with its molecular target by visualizing signal transduction, or assessing the metabolic, physiological, or structural response of the organism studied.The third edition has been extended considerably. This holds for the chapter on imaging modalities, which now includes sections on intravital microscopy and mass spectrometric imaging. All chapters have been updated and a new chapter on the challenges of translating molecular imaging solutions for clinical use has been added.

This thesis describes an in-depth study of an indolizine-based fluorophore, from understanding of its structure-photophysical property relationship to its application as a useful biological reporter.Organic fluorophores have been extensively used in the field of molecular biology owing to their excellent photophysical property, suitable cell permeability, and synthetic flexibility. Understanding of the structure-photophysical property relationship of a given fluorophore often paves the road to the development of valuable molecular probes to visualize and transcribe biological networks. In this thesis, respective chapters deal with molecular design, organic synthesis, structure-property analysis, and quantum-mechanical interpretation of unexplored family of indolizine-based molecules. This systematic exploration has led to rational development of a new microalgae lipid droplet probe, colorful bioorthogonal fluorogenic probes, and a bright mitochondrial probe, working underlive cell conditions.Harnessing the optical properties of a given fluorophore has been an important topic for a couple of decades, both in industry and in academia. This thesis provides useful insights for the improvement and development of unique small fluorescent materials, or optical materials.

This new edition provides the most advanced research using bioprobes on the chemical control of 1) cell cycle and differentiation, 2) epigenetics, 3) apoptosis and autophagy, and 4) immune response. The “bioprobe”, first proposed in the first edition, has become an indispensable tool for chemical biology and has substantially assisted in the investigation of complex biochemical processes of cells. New areas of investigation such as stem cell research, epigenetic research, and autophagy research have rapidly advanced in the past 10 years. Including these new findings, this second edition supplies up-to-date information on the biochemical tools called bioprobes. Data on each bioprobe, such as chemical structure, origin, function, and references, are presented as one item in this volume. Readers will easily find useful information and will be able to determine the appropriate bioprobes to investigate cell functions. The information on bioprobes and their use in research makes this booka valuable source for researchers in diverse fields. Not only scientists in academia but also in the pharmaceutical industries will discover the most important information about small molecules useful for drug discovery.

This volume contains original submissions on the development and application of molecular imaging computing. The editors invited authors to submit high-quality contributions on a wide range of topics including, but not limited to:• Image Synthesis & Reconstruction of Emission Tomography (PET, SPECT) and other Molecular Imaging Modalities• Molecular Imaging Enhancement• Data Analysis of Clinical & Pre-clinical Molecular Imaging• Multi-Modal Image Processing (PET/CT, PET/MR, SPECT/CT, etc.)• Machine Learning and Data Mining in Molecular Imaging.Molecular imaging is an evolving clinical and research discipline enabling the visualization, characterization and quantification of biological processes taking place at the cellular and subcellular levels within intact living subjects. Computational methods play an important role in the development of molecular imaging, from image synthesis to data analysis and from clinical diagnosis to therapy individualization. This work will bring readers from academia and industry up to date on the most recent developments in this field.

Molecular imaging is primarily about the chemistry of novel biological probes, yet the vast majority of practitioners are not chemists or biochemists. This is the first book, written from a chemist's point of view, to address the nature of the chemical interaction between probe and environment to help elucidate biochemical detail instead of bulk anatomy. Covers all of the fundamentals of modern imaging methodologies, including their techniques and application within medicine and industry Focuses primarily on the chemistry of probes and imaging agents, and chemical methodology for labelling and bioconjugation First book to investigate the chemistry of molecular imaging Aimed at students as well as researchers involved in the area of molecular imaging

One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors. Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well as for applications in biomedicine, drug discovery and biotechnology. These tools have been designed and engineered thanks to the combined efforts of chemists and biologists over the last decade, and developed hand in hand together with imaging technologies. This volume will convey the many exciting developments the field of fluorescent biosensors and reporters has witnessed over the recent years, from concepts to applications, including chapters on the chemistry of fluorescent probes, on technologies for monitoring protein/protein interactions and technologies for imaging biosensors in cultured cells and in vivo. Other chapters are devoted to specific examples of genetically-encoded reporters, or to protein and peptide biosensors, together with examples illustrating their application to cellular and in vivo imaging, biomedical applications, drug discovery and high throughput screening. - Contributions from leading authorities - Informs and updates on all the latest developments in the field

The area of molecular imaging has matured over the past decade and is still growing rapidly. Many concepts developed for molecular biology and cellular imaging have been successfully translated to in vivo imaging of intact organisms. Molecular imaging enables the study of processes at a molecular level in their full biological context. Due to the high specificity of the molecular readouts the approach bears a high potential for diagnostics. It is fair to say that molecular imaging has become an indispensable tool for biomedical research and drug discovery and development today.This volume familiarizes the reader with the concepts of imaging and molecular imaging in particular. Basic principles of imaging technologies, reporter moieties for the various imaging modalities, and the design of targeted probes are described in the first part. The second part illustrates how these tools can be used to visualize relevant molecular events in the living organism. Topics covered include the studies of the biodistribution of reporter probes and drugs, visualization of the expression of biomolecules such as receptors and enzymes, and how imaging can be used for analyzing consequences of the interaction of a ligand or a drug with its molecular target by visualizing signal transduction, or assessing the metabolic, physiological, or structural response of the organism studied. The final chapter deals with visualization of cell migration, for example in the context of cell therapies.The second edition covers novel developments over recent years, in particular regarding imaging technologies (hybrid techniques) and novel reporter concepts. Novel biomedical applications have been included, where appropriate. All the chapters have been thoroughly reworked and the artwork updated.

Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications.This book is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice.Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China.Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA.

In this volume, Prof. Ye and his coworkers propose and review the concept of nano-bio probe design for biochemical analysis on the basis of their recent published works. A unique biochemical analysis technology based on fluorescence polarization enhanced by nanoparticles is described here with successful applications in environmental monitoring, rapid and sensitive sensing protease activity and high-throughput screening of inhibitors. Furthermore, they introduce a versatile molecular beacon (MB)-like probe for the multiplex sensing of targets such as sequence-specific DNA, protein, metal ions and small molecule compounds based on the self-assembled biomolecule-graphene conjugates. Besides, some colorimetric and luminescence probes utilizing metal nanoparticles for biochemical applications are also presented, such as chiral enantiomer discrimination and separation, environmental monitoring, clinic diagnosis and etc.

Stem Cell Labeling for Delivery and Tracking Using Noninvasive Imaging provides a comprehensive overview of cell therapy imaging, ranging from the basic biology of cell therapeutic choices to the preclinical and clinical applications of cell therapy. It emphasizes the use of medical imaging for therapeutic delivery/targeting, cell tracking, and det

The ideal starting point for investigating, developing, and implementing stable isotope technologies. • Serves as a valuable resource for environmental microbiology students and researchers and genomics, biotechnology, and medical microbiology professionals.

The cutting-edge guide on advancing the science of molecular imaging using nanoparticles Nanoplathform-Based Molecular Imaging provides rationale for using nanoparticle-based probes for molecular imaging, then discusses general strategies for this underutilized, yet promising, technology. It addresses general strategies of particle synthesis and surface chemistry, applications in computed tomography optical imaging, magnetic resonance imaging, ultrasound, multimodality imaging, theranostics, and finally, the clinical perspectives of nanoimaging. This comprehensive volume summarizes the opinions of those in the forefront of research and describes the latest developments by emphasizing fundamentals and initiating hands-on application.

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and'just look at the thing'as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. - Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Reporter genes have been used for several decades to study regulation of gene expression in vivo. However, it was little more than a decade ago that a new class of reporter genes was developed for imaging molecular events within living subjects. By following the interactions of protein molecules, researchers can resolve the complex chemical pathways that living cells utilise. This book focuses on this group of imaging reporter genes, starting with detailed descriptions of all reporter genes from different imaging modalities, including optical, MRI, and radionuclide-based imaging. Key scientists in the field explain how to enhance reporter gene imaging utility through instrumentation and the various applications of this technology. This is the first comprehensive book on all aspects of reporter gene imaging, detailing what is known in the field and future goals for research. Investigators in biomedical sciences, physicians, and the biotechnology and pharmaceutical industries will benefit from topics covered.

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and'just look at the thing'as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. - Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Discover the link between the latest chemical biology approaches and novel drug therapies! Protein Targeting with Small Molecules: Chemical Biology Techniques and Applications takes readers beyond the use of chemical biology in basic research, providing a highly relevant look at techniques that can address the challenges of biology and drug design and development. This indispensable bench companion features up-to-date coverage of advances in chemistry and assesses their impact on developing new therapeutics, making it ideal for chemical biologists and medicinal chemists who are developing small molecule drugs to target proteins and treat diseases. In addition, the book examines the full range of complex biological systems and their interrelationship with chemistry, from the interaction of biological response modifiers with proteins to the chemical biology of cell surface oligosaccharides. Distinguished by an overview of chemical biology that is reinforced and clarified by detailed examples and descriptions of techniques, Protein Targeting with Small Molecules: Chemical Biology Techniques and Applications: Introduces key technologies and methods of chemical biology designed to detect the interactions of small molecules and proteins Facilitates the discovery of small molecules that bind to proteins and describes the molecules'application in the investigation of biological processes Presents timely coverage of the development of fluorescent probes for small molecules, as well as the generation of small molecule ligands and inhibitors Reviews important techniques such as chemical genomics, target profiling, immobilization technology, detection methods, chemical inhibition, and structure-based targeting Offers a compelling synopsis of data that underscores the recent progress made in the area of targeting proteins by small molecules

The only resource to systematically review current experimental methods, this handy reference enables researchers to select the best solution for their experimental problems. For each method covered, the book provides step-by-step protocols, illustrated by typical research applications. After an initial section on probing the lipid bilayer, the text moves on to discuss probing proteins -- including membrane proteins -- and nucleic acids. The first single publication to incorporate chemical markers, fluorescent probes and genetic tags allows a well-informed comparison of different solutions for the same problem in molecular analytics.

Although fluorescent probes and assays, which use energy transfer (ET) for monitoring DNA reactions, have multiplied in recent years, until now there have been no manuals summarizing the many different protocols and probe designs. Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is the first publication to provide such a collection. In the volume, hands-on experts-often the original developers of the technique-comprehensively describe a variety of fluorescent probes and devices that use ET, including molecular beacons, molecular break lights, TaqMan® probes, LUX primers, Invader® assay, aptazymes, DNAzymes, molecular machines, biosensors, and logic gates for molecular-scale computation. Merging work on nanotechnology and fluorescent probes, the authors assemble the first comprehensive treatment of all the key DNA- and RNA-based ET probes and present strategies for their optimized custom design. They discuss both fluorescence resonance energy transfer (FRET)-based and non-FRET-based constructs, and provide a complete set of techniques to monitor DNA and RNA reactions, such as hybridization, amplification, cleavage, folding, and associations with proteins, other molecules, and metal ions. Detailed protocols are provided for distance determination in protein-DNA complexes and the detection of topological DNA alterations, mutations, and single-nucleotide polymorphisms. The protocols follow the successful Methods in Molecular Biology series format, each offering an outline of the principles behind the technique, step-by-step detailed instructions, and comprehensive troubleshooting. Exhaustive and state-of-the-art, Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is an invaluable resource for both novice and experienced researchers who wish to use the latest developments in fluorescent probes in any field of molecular biology.

Chemical genomics is a highly interdisciplinary and very exciting field of research both in academics and in the life sciences industry. The Ernst Schering Research Foundation Workshop 58 was organized to bring together scientific leaders in the field to discuss the implications of chemical genomics for drug discovery. Various aspects of the interface between chemistry and biology are covered in this volume, such as chemogenomics efforts in the pharmaceutical industry, diversity-oriented synthesis, chemogenomic approaches to the study of cell function, screening technologies, and natural products as tools in chemical biology.

Molecular imaging is a rapidly emerging field that translates many concepts developed for molecular biology and cellular imaging to the in vivo imaging of intact organisms. The technique allows the study of molecular biological events in their full context and will therefore become an indispensable tool for biomedical research and drug discovery and development.This volume familiarizes the reader with the concepts of imaging and molecular imaging in particular. Basic principles of imaging technologies, reporter moieties for the various imaging modalities and the design of target reporter constructs are described in the first part. The second part illustrates how these tools can be used to visualize relevant molecular events: the biodistribution of drugs/ligands, the expression of drug targets (receptors, enzymes), and the consequences of the molecular drug-target interactions (pathway activations, system responses). A final chapter deals with visualization of cell migration (cell therapies).

Chemical genomics is an exciting new field that promises to transform biological chemistry into a high-throughput industrialized process, much in the same way that genomics has transformed molecular biology. In Chemical Genomics: Reviews and Protocols, leading edge researchers introduce the uninitiated into the various'flavors'of chemical genomics and describe a series of readily reproducible techniques for applications in organic chemistry, protein chemistry, and computer science, with emphasis on the high-throughput approaches that are the essence of chemical genomics. The techniques range from the classic design of small molecules as affinity ligands for specific protein families, to in silico docking for computational approaches, to protein-small molecule interactions, to producing highly diverse collections of proteins, carbohydrates, and small molecules for use in arrays containing large numbers of these molecules. There are also methods for the production of proteins and carbohydrates using different expression systems, for analyzing proteins using mass spectrometry, and for arraying proteins and carbohydrates on solid supports. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Chemical Genomics: Reviews and Protocols offers researchers an accessible introduction by pioneers to the key areas and methods of chemical genomics, as well as its use in the discovery and evaluation of novel drug targets.

This state-of-the-art reference provides comprehensive coverage of the development of antisense oligonucleotides to inhibit cancer cells as well as those involved in infectious, inflammatory, and immune-mediated diseases-highlighting new tools and technologies in medicinal chemistry, RNA biochemistry, and molecular and cellular biology to produce new therapeutic compounds.Presents previously unpublished data on the use of antisense technology to dissect pharmacological processes and confirm the roles of various genes!Showcasing the benefits of antisense drug use, including reduced toxicity and earlier disease detection, Antisense Drug Technology discussesnovel formulations of antisense drugs practical methods to design effective isotype selective inhibitors molecular mechanisms of antisense drugs mRNA as a current biological template modern postreceptor binding mechanisms and more!With contributions by over 60 seasoned experts in the field and containing more than 3000 helpful references, tables, drawings, and photographs, Antisense Drug Technology is an illuminating source for organic, medicinal, and pharmaceutical chemists; biochemists; geneticists; hematologists; oncologists; molecular and cell biologists; virologists; immunologists; and medical school and graduate students in these disciplines.

Depuis une dizaine d'années, l'utilisation intensive des marqueurs moléculaires a d'ores et déjà modifié, voire bouleversé, de nombreux secteurs de la biologie. Cet ouvrage fait le point sur les marqueurs moléculaires en génétique formelle, génétique des populations, génétique quantitative et biologie moléculaire. Il définit les concepts, explicite les techniques et recense les références bibliographiques nécessaires pour appréhender et utiliser cet outil désormais irremplaçable. Cette synthèse est centrée sur le domaine végétal. Cependant, comme la plupart des outils et concepts sont communs à tous les organismes pourvus d'ADN, elle sera lue avec profit par tout biologiste ayant recours aux marqueurs, quelle que soit sa problématique.

Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols. - Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules - Gives protocols for nonisotopic DNA sequencing - new in this edition - Gives extensive, practical information - Presents background information for each method - Provides expert accounts from the inventor or developer of each method - Contains seven entirely new chapters - Covers all major types of nonisotopic procedures for labeling and detection