186 results on '"Kuroda, Kouichi"'
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2. Simultaneous Display of Multiple Kinds of Enzymes on the Yeast Cell Surface for Multistep Reactions
3. CRISPR Nickase-Mediated Base Editing in Yeast
4. Overcoming the Limitations of CRISPR-Cas9 Systems in Saccharomyces cerevisiae: Off-Target Effects, Epigenome, and Mitochondrial Editing
5. Foci-forming regions of pyruvate kinase and enolase at the molecular surface incorporate proteins into yeast cytoplasmic metabolic enzymes transiently assembling (META) bodies
6. Preparation of Functional Cells: Improvement of Stress Tolerance
7. Recovery of Rare Metal Ions
8. Energy Production: Biomass – Starch, Cellulose, and Hemicellulose
9. Cleanup of Pollution: Heavy Metal Ions and Environmental Hormones
10. Adaptive Evolution of Yeast Under Heat Stress and Genetic Reconstruction to Generate Thermotolerant Yeast
11. Improving Precise Genome Editing Using Donor DNA/gRNA Hybrid Duplex Generated by Complementary Bases
12. Development of Artificial System to Induce Chromatin Loosening in Saccharomyces cerevisiae
13. A critical role of an oxygen-responsive gene for aerobic nitrogenase activity in Azotobacter vinelandii and its application to Escherichia coli
14. Enzyme Evolution by Yeast Cell Surface Engineering
15. Construction of recombinant Escherichia coli producing nitrogenase-related proteins from Azotobacter vinelandii
16. Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-arming Technology
17. Small‐scale hypoxic cultures for monitoring the spatial reorganization of glycolytic enzymes in Saccharomyces cerevisiae
18. Development of a mito-CRISPR system for generating mitochondrial DNA-deleted strain in Saccharomyces cerevisiae
19. Yeast Biosorption and Recycling of Metal Ions by Cell Surface Engineering
20. A Homogenization-Based Prediction Method of Macroscopic Yield Strength of Polycrystalline Metals Subjected to Cold-Working
21. Efficient ammonia production from food by-products by engineered Escherichia coli
22. Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara)
23. Critical Roles of the Pentose Phosphate Pathway and GLN3 in Isobutanol-Specific Tolerance in Yeast
24. Temporal proteome dynamics of Clostridium cellulovorans cultured with major plant cell wall polysaccharides
25. Direct bioethanol production from brown macroalgae by co-culture of two engineered Saccharomyces cerevisiae strains
26. Cas9ニッカーゼを用いたオフターゲットのない新しいゲノム編集法（CRISPR Nickaseシステム）の開発
27. Xylanase B fromClostridium cellulovorans743B: overexpression, purification, crystallization and X-ray diffraction analysis
28. Platform construction of molecular breeding for utilization of brown macroalgae
29. Engineering of global regulators and cell surface properties toward enhancing stress tolerance in Saccharomyces cerevisiae
30. Characterization of the cellulosomal scaffolding protein CbpC from Clostridium cellulovorans 743B
31. Erratum: Precise genome-wide base editing by the CRISPR Nickase system in yeast
32. Construction of bioengineered yeast platform for direct bioethanol production from alginate and mannitol
33. Development of an Analysis Method for 4-Deoxy-l-erythro-5-hexoseulose Uronic Acid by LC/ESI/MS with Selected Ion Monitoring
34. Precise genome-wide base editing by the CRISPR Nickase system in yeast
35. Enhanced direct ethanol production by cofactor optimization of cell surface-displayed xylose isomerase in yeast
36. Proposal of a numerical model using the finite element analysis of pilger rolling for improvement of computation precision and efficiency
37. Genome Sequence of Formosa haliotis Strain MA1, a Brown Alga-Degrading Bacterium Isolated from the Gut of Abalone Haliotis gigantea
38. Recovery of platinum(0) through the reduction of platinum ions by hydrogenase-displaying yeast
39. Characteristic strategy of assimilation of various saccharides by Clostridium cellulovorans
40. Direct ethanol fermentation of the algal storage polysaccharide laminarin with an optimized combination of engineered yeasts
41. Proposed alginate utilization process of the macroalgae-assimilating Saccharophagus degradans 2-40 based on quantitative proteomic analysis
42. Potentiality of bacterial metagenomes in biorefinery enzyme isolation for brown macroalgae degradation
43. Ethanol production from hemicellulose using xylose isomerase-displaying yeast
44. Precise genome editing at single-base resolution by novel CRISPR-nickase system
45. Platform of direct ethanol production from macroalgae by engineered Saccharomyces cerevisiae
46. The mechanism of organic-solvent tolerance in yeast Saccharomyces cerevisiae triggered by a mutated transcription factor
47. Falsirhodobacter sp. alg1 Harbors Single Homologs of Endo and Exo-Type Alginate Lyases Efficient for Alginate Depolymerization
48. Integrated Yeast‐mammalian Assay System for Screening of Novel Peptides Activating Glucagon‐like Peptide‐1 Receptor
49. Molecular Analysis of Adaptational Strategies under Heat Stress by Whole‐Genome Sequencing of Laboratory‐Evolved Saccharomyces cerevisiae
50. Reconstruction of thermotolerant yeast by one-point mutation identified through whole-genome analyses of adaptively-evolved strains
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