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This study aimed to determine the response of superovulation in pesisir cows. Animals were treated with GnRH at different doses, and we observed the onset, estrous duration, number of CL, and embryos. This study uses a Complete Randomized Design (CRD), with a dose of GnRH administration as a treatment. 16 pesisir cows are used in this study and divided into four groups; each cow must have been pregnant at least once. This research is performed by installing Internal Drug Release Control (CIDR) intravaginal. After the 8th day, each group was injected with GnRH 150 μg, 175 μg, 200 μg, and 225 μg, respectively. On the day – 10th amount of FSH was injected into each cow for 3 days. On the day of – 12th, cows were treated with PGF2α and started to observe estrous signs on day 13th. Artificial insemination was performed using FH (Friesian Holstein) semen. Embryo collection was carried out on days 6 to 8 after AI was performed. Data were analyzed using analysis of variance (ANOVA), and if any significant differences were analyzed with Duncan Multiple Range Test (DMRT). The result showed that the level of GnRH administration has no significant differences in the onset and estrous duration. We also found no significant differences in the amount of CL and embryos. It was be concluded that giving GnRH at different doses had no important on the onset and duration of estrous, number of CL, and the number of embryos. [ABSTRACT FROM AUTHOR]