Search

Copyright of Serbian Dental Journal / Stomatološki Glasnik Srbije is the property of National Library of Serbia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background/purpose: To examine the effects of fibroblast growth factor-2 (FGF-2)/calcium silicate (CS) cement on material characters and in vitro primary human dental pulp cell (hDPC) behavior. Materials and methods: Setting time and diametral tensile strength (DTS) of CS and CS/FGF-2 composite were measured. PrestoBlue assay was used for evaluating primary hDPC proliferation. Alkaline phosphatase and osteocalcin expression in HDPCs cultured on the specimens were determined by enzyme-linked immunosorbent assay. One-way analysis of variance was used to evaluate the significance of the differences between the mean values. Results: Setting time and DTS of CS were not significantly different (P > 0.05) between CS hydration with H2O or FGF-2. Cell proliferation and osteogenic properties increased significantly (P < 0.05) with FGF-2 mixed CS. The CS/FGF-2 composite enhanced hDPC proliferation and osteogenic differentiation as compared to pure CS cement. Conclusion: CS combined with FGF-2 is biocompatible with hDPCs. It not only promotes hDPC proliferation but also helps in differentiating reparative hard tissue. Thus, we suggest that the CS/FGF-2 composite has the potential for hard tissue defect repair. [ABSTRACT FROM AUTHOR]

Background/purpose: The use of root-end filling materials designed to stimulate tissue repair in periradicular tissues is highly recommended. These materials should be proved good tissue compatibility. The aim of this study was to estimate the responses of Intermediate restorative material (IRM), mineral trioxide aggregate (MTA), and calcium silicate (CS) cement after implantation into a rat subcutaneous, including the immune response, and materials degradation. Materials and methods: Materials with the same chemical component were inserted into the bilateral pockets in each rat. Hematoxylin and eosin (HftE) staining was used to evaluate the immune response of tissue after implantation. Western blot was employed to quantify the COX-2 expression of tissue. Results: After implantation for 6 weeks, HEtE staining showed that the inflamed fibrous capsule surrounding MTA and CS was thicker and looser. At 12 weeks, the tissue responses became very uniform for MTA and CS. The capsule was almost free of inflammatory cells and filled with fibroblasts. A significant increase in the thickness of fibrous tissue was observed after 3 weeks, 6 weeks, and 12 weeks for IRM at the respective time points (P < 0.05). Significant increases of 3.45-fold, 2.81-fold, and 2.78-fold in COX-2 synthesis were observed using IRM, as compared with the control, at 3 weeks, 6 weeks, and 12 weeks, respectively (P < 0.05). However, CS cement was found to reduce an inflammatory reaction compared to MTA at all time points (P < 0.05). Conclusion: These three cements could be considered to be biocompatible even though they induced different inflammatory responses and tissue changes during implantation tests in rats. CS cement did not induce acute inflammation and tissue responses similar to those reported for MTA. [ABSTRACT FROM AUTHOR]

Objective: This study aimed to evaluate a prototype surface reaction-type pre-reacted glass-ionomer (S-PRG) filler-containing endodontic sealer (S-PRG sealer) in terms of its ion-releasing ability and the incorporation of the released ions by root canal dentin. Materials and Methods: The concentration of ions in the set S-PRG sealer was measured by ICPS and with a fluoride ion electrode. Element mapping and chemical component bulk analysis of the outermost dentin of the S-PRG sealer-dentin interface were performed by SEM-EPMA. The incorporation depth of the elements in dentin was measured by element line scanning. Results and Discussion: Ions released from the S-PRG sealer (Al, B, F, Na, Si, Sr, and Zn) were detected over time. The incorporation of fluoride (F), strontium (Sr), silicon (Si), and boron (B) was observed along the outermost dentin of the S-PRG sealer-dentin interface by EPMA analysis, and the incorporation depth increased with prolonged immersion. Conclusion: The continuous release of ions from the S-PRG sealer and their subsequent incorporation by root canal dentin were confirmed. [ABSTRACT FROM AUTHOR]

Copyright of Serbian Dental Journal / Stomatološki Glasnik Srbije is the property of National Library of Serbia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Silicone tubes containing a freshly mixed experimental Sealapex, in which titanium dioxide was removed from the original formula, were implanted in the dorsal subcutaneous connective tissue of the rat. Solid silicone rods of the same size as the tubes were also implanted and used as inert controls. The tissue reaction to both these materials was histometrically and quantitatively studied under light microscopy and by elemental electron microprobe analysis. After 7, 30 and 90 d of implantation, different grades of tissue reaction to the tested materials were recorded. A granulomatous tissue containing numerous foreign-body giant cells and macrophages with engulfed particles in their cytoplasms was initially detected in contact with the test material. In addition, many fibroblasts and newly formed vessels were also observed at these areas. The results of this study revealed that these reactions increased progressively at the 30- and 90-d observations. The electron microprobe analysis of the granulomatous tissues showed the presence of heavy components of the experimental material. [ABSTRACT FROM AUTHOR]