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Ovine brucellosis is a global zoonotic disease of sheep caused by Brucella melitensis, which inflicts a significant burden on human and animal health. Brucella suis strain S2 (B. suis S2) is a smooth live attenuated vaccine for the prevention of ovine brucellosis in China. However, no previous studies have assessed the immunogenicity of B. suis S2 vaccine after oral immunization in sheep. Here, we attempted to evaluate the ovine immune response over the course of B. suis S2 immunization and to identify in vivo predictors for vaccine development. Body temperature, serum Brucella antibodies, serum cytokines (IL-12p70 and interferon [IFN]-γ), and bacterial load in the mandibular lymph nodes (LN), superficial cervical LN, superficial inguinal LN, and spleen were investigated to determine the safety and efficacy of the vaccine. The abnormal body temperature of sheep occurred within 8 days post-infection (dpi). Brucella suis S2 persisted for a short time (< 21 dpi) in the mandibular LN. The highest level of IL-12p70 was observed at 9 dpi, whereas serum IFN-γ levels peaked at 12 dpi. Transcriptome analysis and quantitative reverse transcription PCR were performed to determine gene expression profiles in the mandibular LN of sheep. Antigen processing and presentation pathway was the dominant pathway related to the dataset. Our studies suggest that the immune response in ovine LN resembled type 1 immunity with the secretion of IL-12p70 and IFN-γ after B.suis S2 immunization and the vaccine may eliminate Brucella via stimulation of M1 macrophages through the course of Th cells. [ABSTRACT FROM AUTHOR]

• The study uses DEA and LCA to optimize sheep farming production. • Energy use in sheep farming can be reduced by 9.82% with optimization. • Food production is responsible for 69% of the environmental impact. • Optimization in sheep farming can save 4677.64 MJ of energy. • Greenhouse gas emissions can be reduced with optimization strategies. Over the past decades, the global population has significantly increased, resulting in high demand for food. China, the largest sheep-producing country, has experienced 20 rapid expansions in its sheep industry, which has caused environmental consequences. This study proposes an integrated approach that combines data envelopment analysis (DEA) and life cycle assessment (LCA) to assess the ecological consequences of the sheep production system. Therefore, this work aims to evaluate the energy consumption of the existing production system and identify optimization opportunities to reduce its environmental impacts. Our results demonstrate that sheep production can decrease total energy consumption by 9.82% using optimization. Food production was reported as a critical factor in environmental impact, responsible for 69%. However, the optimized approach led to a decrease of 4,677.64 MJ in energy consumption in sheep production compared to the initial state. The findings of this study showcase the possibility of decreasing energy usage and lessening greenhouse gas (GHG) discharges in sheep farming by implementing optimization strategies. [Display omitted] [ABSTRACT FROM AUTHOR]