Search

Objective:The presence of microorganisms in a wound may lead to the development of pathologically extensive inflammation, and either delay or prevent the healing of hard-to-heal (chronic) wounds. The aim of this case series is to explore the use of topical gentamicin ointment, an aminoglycoside with activity against aerobic Gram-negative bacteria, as an option to address hard-to-heal wounds.Method:We present a retrospective case series of patients with hard-to-heal wounds of varying pathophysiologies treated with topical gentamicin.Results:Regardless of the inciting aetiology, all 10 of the presented patients responded to topical gentamicin as evidenced by resolution of much of the inflammation and reduction in wound size or complete wound closure.Conclusion:By helping to clear the bacteria-induced biofilm that may be present on a hard-to-heal wound, gentamicin may allow the wound healing process to continue past the inflammatory phase, and onto proliferation, remodelling and eventual closure.

The first ONTOX Hackathon of the EU Horizon 2020-funded ONTOX project was held on 21–23 April 2024 in Utrecht, The Netherlands (https://ontox-project.eu/hackathon/). This participatory event aimed to collectively advance innovation for human safety through the use of Artificial Intelligence (AI), and hence significantly reduce reliance on animal-based testing. Expert scientists, industry leaders, young investigators, members of animal welfare organisations and academics alike, joined the hackathon. Eight teams were stimulated to find innovative solutions for challenging themes, that were selected based on previous discussions between stakeholders, namely: How to drive the use of AI in chemical risk assessment?; To predict or protect?; How can we secure human health and environmental protection at the same time?; and How can we facilitate the transition from animal tests to full implementation of human-relevant methods? The hackathon ended with a pitching contest, where the teams presented their solutions to a jury. The most promising solutions will be presented to regulatory authorities, industry, academia and non-governmental organisations at the next ONTOX Stakeholder Network meeting and taken up by the ONTOX project in order to tackle the above-mentioned challenges further. This report comprises two parts: The first part highlights some of the lessons learnt during the planning and execution of the hackathon; the second part presents the outcome of the ONTOX Hackathon, which resulted in several innovative and promising solutions based on New Approach Methodologies (NAMs), and outlines ONTOX’s intended way forward.

Aims/BackgroundSymptomatic abdominal aortic aneurysms carry significant mortality risk. This is supplemented by the Royal College of Emergency Medicine guidelines which suggest imaging for patients 50 years of age or older presenting with unexplained abdominal, flank, or back pain. This study aimed to evaluate the prevalence and mortality rates of patients with symptomatic abdominal aortic aneurysms in a high-risk population and to assess scanning rates in the accident and emergency department.MethodsRetrospective analysis of patients presenting to the accident and emergency department at a district general hospital over 6 months was performed. Patients 50 years of age or older presenting with abdominal, flank, or back pain were included. Collected data points included; whether or not a scan was performed in the emergency department, the modality of imaging, whether an abdominal aortic aneurysm was identified on the scan, the age of patients with an abdominal aortic aneurysms identified on the scan, size of the identified abdominal aortic aneurysms, primary diagnosis at the time of review in the emergency department, and all-cause mortality rates.Results361 patients were identified to have an indicated scan, of which only 122 (33.8%) had a scan in the emergency department. In the syndromic group, the prevalence and 30-day mortality of patients with an abdominal aortic aneurysm were 5.5% and 1.1% respectively. Only 12 out of 20 patients with an abdominal aortic aneurysm were identified in the emergency department.ConclusionThe criteria outlined by the Royal College of Emergency Medicine does well at identifying patients with abdominal aortic aneurysms when followed. However, this study reveals that scanning rates in the emergency department are low. The encouragement of scanning and improved ultrasound skills among emergency medicine clinicians can reduce missed diagnoses. Additionally, we recommend further studies to assess the mortality rates of emergent abdominal aortic aneurysm presentations.

Ultrafast stimuli can stabilize metastable states of matter inaccessible by equilibrium means. Establishing the spatiotemporal link between ultrafast excitation and metastability is crucial to understand these phenomena. Here we utilize single-shot optical pump–X-ray probe measurements to capture snapshots of the emergence of a persistent polar vortex supercrystal in a heterostructure that hosts a fine balance between built-in electrostatic and elastic frustrations by design. By perturbing this balance with photoinduced charges, an initially heterogeneous mixture of polar phase disorders within a few picoseconds, leading to a state composed of disordered ferroelectric and suppressed vortex orders. On the picosecond–nanosecond timescales, transient labyrinthine fluctuations develop, accompanied by the recovery of the vortex order. On longer timescales, these fluctuations are progressively quenched by dynamical strain modulations, which drive the collective emergence of a single vortex supercrystal phase. Our results, corroborated by dynamical phase-field modelling, reveal non-equilibrium pathways following the ultrafast excitation of designer systems to persistent metastability.

Prior research on the internationalization of firms from emerging countries has fruitfully invoked institutional theory to emphasize the legitimacy benefits that firms that obtain from showing isomorphism with international norms such as Corporate Social Responsibility (CSR). Without denying the intuitive appeal for these firms to communicate acceptance of CSR, we suggest that firms face a legitimacy trade‐off, where the hoped‐for legitimacy benefits of isomorphism must be weighed against other home‐country institutional considerations. We advance and test this notion that firms will navigate this institutional complexity by engaging in anisomorphism, i.e., espousing general acceptance with international values but with selective ‘translation’ based on home country differences. We test our predictions by analysing firms' communication of CSR, using a unique dataset comprised of 245 firms observed over the period from 2000 to 2018. Consistent with our predictions, we find that firms from countries more reliant on natural resource extraction (e.g., mining and fossil fuel industries) de‐emphasize the environmental component of CSR, and firms from more autocratic countries de‐emphasize the human rights component of CSR. Additionally, and consistent with our presumption of firms' weighing the international versus home‐country legitimacy trade‐off, we find that these main effects are sensitive to changes in firms' levels of internationalization.

This study extends prior research on corporate political behaviour (CPB) and firms’ pursuit of political legitimacy in response to monolithic government pressures by developing and testing a framework for analysis of CPB in response to polylithic pressures. We suggest that traditional forms of CPB may be ill‐suited to polylithic governmental pressures, such as when firms need to navigate between conflicting home‐ and host‐country political worldviews and policies. We posit that in such complex political situations, firms will turn to a more subtle form of CPB (i.e., rhetorical commitment versus avoidance) as a hoped‐for solution to their international political legitimacy challenge. Our contingency perspective also highlights how geopolitical factors (i.e., whether governments of home and host countries are clearly aligned versus misaligned) will influence whether firms express their support for a home government’s foreign policy or avoid any such expression of support. We empirically test the predictive power of our framework by analysing how these political factors led Chinese firms to opt for rhetorical commitment versus rhetorical avoidance vis‐à‐vis the Chinese government’s Belt and Road Initiative (BRI). We conclude with a discussion of how our framework for analysis and our supportive findings can inform and extend research on CPB and political legitimacy.

The review aimed to illustrate the structure, role and effects of omega-3 polyunsaturated fatty acids (omega-3 PUFAs) in combination with acetylsalicylic acid (aspirin, ASA) in various clinical cases. This verification of earlier single studies may be a guide in the further treatment of civilization diseases. The results of the presented narrative review suggest that aspirin supplementation with omega-3 fatty acids reduces pro-inflammatory biomarkers in sepsis and acute respiratory distress syndrome. Including adequate amounts of omega 3-PUFAs in therapy, rather than increasing the dose of acetylsalicylic acid, may contribute to beneficial effects in treating thrombosis and preventing myocardial infarction or other cardiovascular diseases, which is particularly important in aspirin-resistant patients. As suggested in the literature, a low daily dose of omega-3 fatty acids was effective in slowing the progression of kidney disease with IgA nephropathy. Aspirin supplementation with omega-3 fatty acids has improved clinical and immunological outcomes in the treatment of periodontitis or the therapy of colorectal cancer tumor lesions. The synergistic effect of both compounds is proposed as a new therapeutic option in the treatment of Parkinson’s disease. Further research into the synergistic effects of omega-3 fatty acids in combination with acetylsalicylic acid may provide a breakthrough in drug dose reduction, ultimately enabling more effective and safer pharmacotherapy for the patient. Therefore, studies on polyunsaturated fatty acids in combination with nonsteroidal anti-inflammatory drugs in vivoare needed.

The first Stakeholder Network Meeting of the EU Horizon 2020-funded ONTOX project was held on 13–14 March 2023, in Brussels, Belgium. The discussion centred around identifying specific challenges, barriers and drivers in relation to the implementation of non-animal new approach methodologies (NAMs) and probabilistic risk assessment (PRA), in order to help address the issues and rank them according to their associated level of difficulty. ONTOX aims to advance the assessment of chemical risk to humans, without the use of animal testing, by developing non-animal NAMs and PRA in line with 21st century toxicity testing principles. Stakeholder groups (regulatory authorities, companies, academia, non-governmental organisations) were identified and invited to participate in a meeting and a survey, by which their current position in relation to the implementation of NAMs and PRA was ascertained, as well as specific challenges and drivers highlighted. The survey analysis revealed areas of agreement and disagreement among stakeholders on topics such as capacity building, sustainability, regulatory acceptance, validation of adverse outcome pathways, acceptance of artificial intelligence (AI) in risk assessment, and guaranteeing consumer safety. The stakeholder network meeting resulted in the identification of barriers, drivers and specific challenges that need to be addressed. Breakout groups discussed topics such as hazard versus risk assessment, future reliance on AI and machine learning, regulatory requirements for industry and sustainability of the ONTOX Hub platform. The outputs from these discussions provided insights for overcoming barriers and leveraging drivers for implementing NAMs and PRA. It was concluded that there is a continued need for stakeholder engagement, including the organisation of a ‘hackathon’ to tackle challenges, to ensure the successful implementation of NAMs and PRA in chemical risk assessment.

In this study, various techniques, such as energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Raman spectroscopy, and spectrophotometry, were used to analyze the properties of nanometric CdS particles synthesized with varying precursor concentrations. EDX analysis revealed the nonstoichiometric composition manifested by an increase in the Cd/S ratio from 1.02 up to 1.43 with increasing precursor concentration. The growth of lattice parameters and unit cell volume accompanying preferential crystallization of the hexagonal phase along with an increasing Cd/S ratio was confirmed by XRD analysis. This indicated the presence of interstitial cadmium in nonstoichiometric Cd1+xS. The formation of shallow Cdidonor levels below the bottom edge of the conduction band impacts the bang-gap energy; a decrease from 2.56 to 2.21 eV along with increasing nonstoichiometry was observed. This is accompanied by a widening of the range of absorption of light, which creates conditions that can lead to an increase in the efficiency of redox reactions in photochemical processes.

AbstractThis study explored composite cements consisting of Portland clinker, two pozzolans (alumina-silica gel in carbonated Recycled Concrete Paste (cRCP) and metakaolin), and calcium carbonate. The rapid reactivity of alumina-silica gel resulted in full reaction within 28 days, while metakaolin exhibited slower reactivity. cRCP played a significant role in strength development between 1 and 7 days, with metakaolin becoming prominent thereafter. The combination of materials preserved their distinct characteristics. Early hydration was mainly influenced by clinker and cRCP, leading to high early compressive strength. After alumina-silica gel dissolved, metakaolin forms a microstructure, reducing porosity and allowing for continued strength improvement over time. The pozzolans did not hinder their respective reactions, and calcium carbonate from cRCP stabilized ettringite content, similar to limestone-calcined clay systems. This study’s findings offer insights for developing multi-component cement with cRCP and calcined clays, resulting in higher strength and reduced CO2 footprint compared to calcined clay-limestone cements.

Solid-state devices made from correlated oxides, such as perovskite nickelates, are promising for neuromorphic computing by mimicking biological synaptic function. However, comprehending dopant action at the nanoscale poses a formidable challenge to understanding the elementary mechanisms involved. Here, we perform operandoinfrared nanoimaging of hydrogen-doped correlated perovskite, neodymium nickel oxide (H-NdNiO3, H-NNO), devices and reveal how an applied field perturbs dopant distribution at the nanoscale. This perturbation leads to stripe phases of varying conductivity perpendicular to the applied field, which define the macroscale electrical characteristics of the devices. Hyperspectral nano-FTIR imaging in conjunction with density functional theory calculations unveils a real-space map of multiple vibrational states of H-NNO associated with OH stretching modes and their dependence on the dopant concentration. Moreover, the localization of excess charges induces an out-of-plane lattice expansion in NNO which was confirmed by in situX-ray diffraction and creates a strain that acts as a barrier against further diffusion. Our results and the techniques presented here hold great potential for the rapidly growing field of memristors and neuromorphic devices wherein nanoscale ion motion is fundamentally responsible for function.

Abstract.The Rational-Experiential Multimodal Inventory (REIm) is a recent tool showing promise in the measurement of self-reported thinking style – preference for rational or experiential processing – and offers three-faceted measurement of the latter. We present the first short form of the measure, the REIm-13, and test its factor structure, reliability, and validity in a large community sample. Participants were N= 920 Australian adults (502 females) who completed an online survey (N= 510 of whom participated in a follow-up survey). In addition to the REIm, participants completed a Big Five personality measure. The internal consistency of the REIm-13 was acceptable given the limited number of items (.52–.68). Furthermore, test-retest reliability was high (ρ = .64–.74) for the theorized four-factor and two-factor solutions. Construct validity was established by examining the relationship between short-form and full REIm factors (ρ = .65–.71), and the validity of composite scoring was confirmed against factor scoring. Relationship with age (a slight negative relationship to both main scales), gender (females reporting higher Experientiality, males higher Rationality), and Big Five variables largely followed previous findings, demonstrating concurrent validity. The study demonstrates that the REIm-13 provides sound measurement of thinking style.

Understanding the origin of electron–phonon coupling in lead halide perovskites is key to interpreting and leveraging their optical and electronic properties. Here we show that photoexcitation drives a reduction of the lead–halide–lead bond angles, a result of deformation potential coupling to low-energy optical phonons. We accomplish this by performing femtosecond-resolved, optical-pump–electron-diffraction-probe measurements to quantify the lattice reorganization occurring as a result of photoexcitation in nanocrystals of FAPbBr3. Our results indicate a stronger coupling in FAPbBr3than CsPbBr3. We attribute the enhanced coupling in FAPbBr3to its disordered crystal structure, which persists down to cryogenic temperatures. We find the reorganizations induced by each exciton in a multi-excitonic state constructively interfere, giving rise to a coupling strength that scales quadratically with the exciton number. This superlinear scaling induces phonon-mediated attractive interactions between excitations in lead halide perovskites.

ObjectivesThe objectives of this study are to assess reporting of evidence-based healthcare (EBHC) e-learning interventions using the Guideline for Reporting Evidence-based practice Educational interventions and Teaching (GREET) checklist and explore factors associated with compliant reporting.DesignMethodological cross-sectional study.MethodsBased on the criteria used in an earlier systematic review, we included studies comparing EBHC e-learning and any other form of EBHC training or no EBHC training. We searched Medline, Embase, ERIC, CINAHL, CENTRAL, SCOPUS, Web of Knowledge, PsycInfo, ProQuest and Best Evidence Medical Education up to 4 January 2023. Screening of titles, abstracts, full-text articles and data extraction was done independently by two authors. For each study, we assessed adherence to each of the 17 GREET items and extracted information on possible predictors. Adequacy of reporting for each item of the GREET checklist was judged with yes (provided complete information), no (provided no information), unclear (when insufficient information was provided), or not applicable, when the item was clearly of no relevance to the intervention described (such as for item 8—details about the instructors—in the studies which used electronic, self-paced intervention, without any tutoring). Studies’ adherence to the GREET checklist was presented as percentages and absolute numbers. We performed univariate analysis to assess the association of potential adherence predictors with the GREET checklist. We summarised results descriptively.ResultsWe included 40 studies, the majority of which assessed e-learning or blended learning and mostly involved medical and other healthcare students. None of the studies fully reported all the GREET items. Overall, the median number of GREET items met (received yes) per study was 8 and third quartile (Q3) of GREET items met per study was 9 (min. 4 max. 14). When we used Q3 of the number of items met as cut-off point, adherence to the GREET reporting checklist was poor with 7 out of 40 studies (17.5%) reporting items of the checklist on acceptable level (adhered to at least 10 items out of 17). None of the studies reported on all 17 GREET items. For 3 items, 80% of included studies well reported information (received yes for these items): item 1 (brief description of intervention), item 4 (evidence-based practice content) and item 6 (educational strategies). Items for which 50% of included studies reported complete information (received yes for these items) included: item 9 (modes of delivery), item 11 (schedule) and 12 (time spent on learning). The items for which 70% or more of included studies did not provide information (received no for these items) included: item 7 (incentives) and item 13 (adaptations; for both items 70% of studies received no for them), item 14 (modifications of educational interventions—95% of studies received no for this item), item 16 (any processes to determine whether the materials and the educational strategies used in the educational intervention were delivered as originally planned—93% of studies received no for this item) and 17 (intervention delivery according to schedule—100% of studies received no for this item). Studies published after September 2016 showed slight improvements in nine reporting items. In the logistic regression models, using the cut-off point of Q3 (10 points or above) the odds of acceptable adherence to GREET guidelines were 7.5 times higher if adherence to other guideline (Consolidated Standards of Reporting Trials, Strengthening the Reporting of Observational Studies in Epidemiology, etc) was reported for a given study type (p=0.039), also higher number of study authors increased the odds of adherence to GREET guidance by 18% (p=0.037).ConclusionsStudies assessing educational interventions on EBHC e-learning still poorly adhere to the GREET checklist. Using other reporting guidelines increased the odds of better GREET reporting. Journals should call for the use of appropriate use of reporting guidelines of future studies on teaching EBHC to increase transparency of reporting, decrease unnecessary research duplication and facilitate uptake of research evidence or result.Study registration numberThe Open Science Framework (https://doi.org/10.17605/OSF.IO/V86FR).

CO2curing of fresh concrete is one of the solutions allowing direct carbon sequestration within the construction industry. Additionally, it enhances the early mechanical performance when comparing to traditional hydration curing. Temperature of carbonation curing has pronounced impact on both reactions involved in hardening of Portland cement: carbonation and hydration. Both reactions are accelerated with rising temperature, while the formed products and resulting microstructure are altered. Higher curing temperature promotes the precipitation of silica gel and C-S-H phase with reduced Ca/Si ratio. Furthermore, alumina and sulfate distributions among the reaction products are different. Temperature of carbonation curing has a distinct impact on the cement paste hydration after carbonation curing. The high curing temperature results in a densification of the matrix which limits further reaction progress, compared to the samples cured at lower temperatures. During the post hydration, calcium deficient system changes into C-S-H phase with higher Ca/Si and eventually portlandite.

Constant, unrelieved pressure of local tissue, particularly over bony prominences, may provoke damage that progresses to necrosis and pressure injury (PI). Differentiating PIs from conditions of similar appearance is imperative to minimising complications and implementing prompt treatment. This case series describes several conditions that may be mistaken for a PI. Outlined are the key differences in patient history, presentation and clinical cues that assist in correctly identifying the true pathology behind these conditions. Conditions reviewed included: pyoderma gangrenosum; necrotising fasciitis; genital herpes; Marjolin ulcer, Rosai–Dorfman disease; vascular disease; coagulopathies; calciphylaxis; trauma and surgical wounds; pilonidal cysts; graft-versus-host disease; hidradenitis suppurativa; Stevens–Johnson syndrome; epidermolysis bullosa; radiation wounds; spider bites; and end-of-life skin failure pressure ulcers (also known as Kennedy ulcers). Although commonly recognised and diagnosed, stage 2, 3 and 4 PIs occasionally prove to be difficult to pinpoint, with undefined characteristics and similarities in presentation to several other conditions. Therefore, it is clinically vital to be aware of their appearance, risk factors and aetiology in order to make an appropriate patient assessment and avoid misdiagnosis.

The institutionalized status of markets is undoubtedly due to their presumed ability to aggregate individual bids into a single unbiased estimate of value. While not denying this emergent property of market processes, we propose and test an alternative perspective that explains how market processes can also generate the propagation of individual valuation errors that aggregate into price bubbles. Theoretically, we advance a microinstitutional perspective that draws from social and evolutionary psychology linking market processes to a more general process of institutionalization, whereby individuals seeking the adaptive benefits of conformity may—due to bounded and socially biased rationality—instead generate maladaptive individual and collective outcomes. Empirically, we craft an efficient experimental market and find three sets of evidence consistent with our microinstitutionalization perspective. We first show—at the individual level—that market participants exhibit a social bias toward conformity with the market’s collective valuation, even when the emergent market valuation is demonstrably incorrect. We then show—at the market level—that the range of valuations over time also decreases in a conforming direction, again independent of valuation accuracy. Last, we provide the first experimental test of the long-assumed effect of social ambiguity on institutionalization, finding that market participants’ over-attention to the collective valuation is indeed sensitive to variation in social ambiguity. We conclude by highlighting the relevance of our theoretical perspective, method, and findings for future research on institutions and institutionalization processes, as well as future studies on social influence and conformity-based errors.Funding:S. S. Levine acknowledges research grants from Singapore Management University; the University of Texas at Dallas; and the European Research Council (agreement 695256).Supplemental Material:The online appendix is available at https://doi.org/10.1287/stsc.2022.0173.

Krzysztofik, M, Wilk, M, Pisz, A, Kolinger, D, Bichowska, M, Zajac, A, and Stastny, P. Acute effects of high-load versus plyometric conditioning activity on jumping performance and the muscle-tendon mechanical properties. J Strength Cond Res37(7): 1397–1403, 2023—The effectiveness of high-load and plyometric exercises as conditioning activity (CA) is not well described in the level of performance enhancement and muscle-tendon properties. Therefore, this study aimed to compare the effectiveness of high-loaded back squats and body mass tuck jumps among amateur soccer players on the height of countermovement jump performed without (CMJ) and with arm swing (CMJa) and to verify the usefulness of the myotonometry in assessing the level of CA-induced fatigue. Therefore, 16 male amateur soccer players (resistance training experience: 2 ± 1 year, relative 1 repetition maximum back squat strength: 1.41 ± 0.12 kg·body mass−1) performed 3 experimental sessions to compare the acute effects of 3 sets of 3 repetitions at 85% one repetition maximum of half back squats (HL), 3 sets of 5 repetitions of tuck jump exercises (PLY), and no CA (CTRL) on CMJ and CMJa height. Moreover, the gastrocnemius medialis and Achilles tendon tone and stiffness were examined. Measurements were performed 5 minutes before CA and in the third, sixth, and ninth minutes after CA. The CMJ height significantly increased from pre-CA to post-CA in the CTRL (p= 0.005; effect size [ES] = 0.36; Δ = +3.4%) and PLY (p= 0.001; ES = 0.83; Δ = +8.8%) conditions. Moreover, post-CA jump height was significantly higher in PLY than in the HL condition (p= 0.024; ES = 0.6; Δ = +5.9%). No significant differences were found for CMJa height, tone, and stiffness of gastrocnemius medialis and Achilles tendon. The low-volume plyometric CA (i.e., 3 sets of 5 repetitions) is recommended instead of high-loaded CA (≥85% one repetition maximum) for amateur athletes. In addition, it has been established that the performance improvement was independent of changes in the mechanical properties of the gastrocnemius medialis and Achilles tendon. Furthermore, it seems that the complexity of the post-CA task may affect the magnitude of the postactivation performance enhancement.

We previously showed that elevated TYMS exhibits oncogenic properties and promotes tumorigenesis after a long latency, suggesting cooperation with sequential somatic mutations. Here we report the cooperation of ectopic expression of human TYMS with loss of Ink4a/Arf, one of the most commonly mutated somatic events in human cancer. Using an hTS/Ink4a/Arf−/−genetically engineered mouse model we showed that deregulated TYMS expression in Ink4a/Arfnull background accelerates tumorigenesis and metastasis. In addition, tumors from TYMS-expressing mice were associated with a phenotype of genomic instability including enhanced double strand DNA damage, aneuploidy and loss of G1/S checkpoint. Downregulation of TYMS in vitro decreased cell proliferation and sensitized tumor cells to antimetabolite chemotherapy. In addition, depletion of TYMS in vivo by TYMS shRNA reduced tumor incidence, delayed tumor progression and prolonged survival in hTS/Ink4a/Arf−/−mice. Our data shows that activation of TYMS in Ink4a/Arfnull background enhances uncontrolled cell proliferation and tumor growth, supporting the development of new agents and strategies targeting TYMS to delay tumorigenesis and prolong survival.

Nitrification is considered one of the most temperature-sensitive biological steps in wastewater treatment. Nitrifying bacteria are highly sensitive to temperature drops, resulting in a rapid decrease in their activity. This study compares the effect of a rapid temperature decrease on the ammonia oxidation rate (AOR) and nitrite oxidation rate (NitOR), with consideration of the form in which biomass develops in the IFAS-MBSBBR. Ammonia Utilisation Rate Tests and Nitrite Utilisation Rate Tests were conducted for two temperatures, namely 20°C and 12°C, for the following forms of biomass: activated sludge (AS), biofilm (B), and combination of both – hybrid (H). The tests showed that nitrite oxidising bacteria inhabiting biofilm were more sensitive to a rapid temperature change than those in activated sludge. A sudden drop of temperature caused a 15% higher than predicted decrease in AOR for AS. At 12°C, AOR changed more considerably than NitOR in tests carried out for H. A temperature correction coefficient of 1.107–1.087 was proposed, applicable in hybrid wastewater treatment systems. Microbiological analysis shows that nitrifiers occurred more abundantly in biofilm than in activated sludge.