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The Beibu Gulf has experienced blooms of Phaeocystis globosa "giant colony" ecotype (PGGCE), with noticeable variations in bloom scale across years. However, driving environmental factors and their roles remain poorly understood. In this study, we quantified dynamics of PGGCE cells in 2016–2017 and 2018–2019, and analyzed their correlations with environment factors. The results revealed that PGGCE blooms primarily occurred in Guangxi coast and western waters of Leizhou Peninsula during winter months, exhibiting distinct developmental processes. Bloom intensity, duration, and distribution differed significantly between two bloom events. In 2016–2017, peak PGGCE density exceeded 2.0 × 105 cells L−1 nearly double that of 2018–2019. Furthermore, bloom sustained five months during 2016–2017, compared to three months during 2018–2019. Prolonged period of low temperatures and elevated nitrate concentrations favored PGGCE growth and colony formation, resulting in a larger scale bloom during winter 2016 as opposed to winter 2018. • Phaeocystis globosa "giant-colony" ecotype (PGGCE) blooms outbreak in the Beibu Gulf. • PGGCE blooms in the Gulf exhibit distinct developmental processes. • Variations of bloom scales in PGGCE blooms across years. • Multiple environmental factors affect PGGCE blooms in the Gulf to varying degrees. • Prolonged period of low temperatures and high nitrate concentrations sustain large scale of PGGCE blooms in the Gulf. [ABSTRACT FROM AUTHOR]

Lipophilic marine toxins in shellfish pose significant threats to the health of seafood consumers. To assess the contamination status of shellfish by lipophilic marine toxins in the Bohai Sea, nine species of shellfish periodically collected from five representative aquaculture zones throughout a year were analyzed with a method of liquid chromatography-tandem mass spectrometry (LC–MS/MS). Lipophilic marine toxins, including okadaic acid (OA), dinophysistoxin-1 (DTX1), pectenotoxin-2 (PTX2), yessotoxin (YTX), homo-yessotoxin (homo-YTX), azaspiracids (AZA2 and AZA3), gymnodimine (GYM), and 13-desmethyl spirolide C (13-DesMe-C), were detected in more than 95 percent of the shellfish samples. Toxins PTX2, YTX, 13-DesMe-C and GYM were predominant components detected in shellfish samples. Scallops, clams and mussels accumulated much higher level of lipophilic marine toxins compared to oysters. Toxin content in shellfish samples collected from different sampling locations showed site-specific seasonal variation patterns. High level of toxins was found during the stages from December to February and June to July in Hangu, while from March to April and August to September in Laishan. Some toxic algae, including Dinophysis acuminata , D. fortii , Prorocentrum lima , Gonyaulax spinifera and Lingulodinium polyedrum , were identified as potential origins of lipophilic marine toxins in the Bohai Sea. The results will offer a sound basis for monitoring marine toxins and protecting the health of seafood consumers. Image 1 • Shellfish samples were monthly collected from aquaculture zones in the Bohai Sea. • Lipophilic phycotoxins in shellfish were analyzed using HPLC-MS/MS combined with SPE. • Lipophilic phycotoxins could be detected in 104 out of 110 shellfish samples. • PTX2, YTX, SPX1 and GYM were predominated in shellfish samples from the Bohai Sea. • Scallop, clam and mussel were easily contaminated by lipophilic phycotoxins. A wide array of lipophilic marine toxins were detected in shellfish samples from the Bohai Sea, which constitute an emerging pollution issue in this region. [ABSTRACT FROM AUTHOR]

Recently, dinoflagellate blooms have frequently occurred in the coastal waters of Fujian, East China Sea. In June 2022, a fish-killing bloom of Kareniaceae species occurred in this region. In this study, four species of Kareniaceae, namely, Karenia longicanalis , K. papilionacea , Karlodinium veneficum , and Karl. digitatum were identified from this bloom event based on the results of single-cell PCR and clone libraries, and intraspecies genetic diversity was found in the Karl. veneficum population. The results of acute toxicity assays of the bloom water to two zooplankton species (Brachionus plicatilis and Artemia salina) demonstrated this bloom event strongly inhibited their swimming capacities and survival. The results of this study suggested that the bloom events caused by multiple species of Kareniaceae in the Fujian coastal waters had adverse impacts on the local fishery resources and zooplankton community. • A fish-killing bloom of Kareniaceae multiple species outbroke in Fujian coast in June 2022. • The bloom was dominated by Karenia longicanalis , K. papilionacea , Karlodinium veneficum , and Karl. digitatum. • Karl. veneficum population has intraspecies genetic diversity. • The bloom strongly inhibited the survival of Brachionus plicatilis and Artemia salina. [ABSTRACT FROM AUTHOR]

Phytoplankton and shellfish samples collected periodically from 5 representative mariculture zones around the Bohai Sea, Laishan (LS), Laizhou (LZ), Hangu (HG), Qinhuangdao (QHD) and Huludao (HLD), were analysed for paralytic shellfish toxins (PSTs) using an high-performance liquid chromatography (HPLC) method. Toxins were detected in 13 out of 20 phytoplankton samples, and N -sulfocarbamoyl toxins (C1/2) were predominant components of PSTs in phytoplankton samples with relatively low toxin content. However, two phytoplankton samples with high PST content collected from QHD and LS had unique toxin profiles characterized by high-potency carbamoyl toxins (GTX1/4) and decarbamoyl toxins (dcGTX2/3 and dcSTX), respectively. PSTs were commonly found in shellfish samples, and toxin content ranged from 0 to 27.6 nmol/g. High level of PSTs were often found in scallops and clams. Shellfish from QHD in spring, and LZ and LS in autumn exhibited high risks of PST contamination. [ABSTRACT FROM AUTHOR]

The dinoflagellate genus Alexandrium comprises most of the toxic bloom-forming species producing paralytic shellfish toxins (PSTs) in the sea. Recently, repeated paralytic shellfish poisoning episodes have been recorded in Qinhuangdao located at the west coast of the Bohai Sea. To elucidate the relationship between toxic Alexandrium blooms and the poisoning episodes, a year-round investigation was carried out in this region from July 2020 to July 2021. Two qPCR assays were used to detect A. catenella and A. pacificum , and LC-MS/MS was applied to analyze PSTs in phytoplankton and shellfish samples. The blooms of A. catenella and A. pacificum were found in April and July, respectively, and PST content in three bivalves exhibited notable increase following the bloom of A. catenella. The results revealed bloom dynamics of the two toxic Alexandrium species in the Bohai Sea for the first time, and further confirmed A. catenella as the causative agent of poisoning episodes. • Toxic Alexandrium and paralytic shellfish toxins were studied in Qinhuangdao, China. • catenella bloomed in April and A. pacificum bloomed in July based on qPCR assay. • Toxin content in bivalves had notable increase following the bloom of A. catenella. • catenella was confirmed as causative agent for poisoning episodes in this region. [ABSTRACT FROM AUTHOR]

This study characterizes the distribution of two closely related, causative species of paralytic shellfish poisoning – Alexandrium fundyense and A. pacificum – within the Yellow Sea (YS) and Bohai Sea (BS). These two Alexandrium species are distinguished for the first time in a regional field study using species-specific, quantitative PCR (qPCR) based assays. Both qPCR assays target the large subunit ribosomal DNA gene and were used to analyze net-concentrated phytoplankton samples collected in May 2012. A. fundyense was mainly distributed in YS, while A. pacificum was confined to an area adjacent to the Changjiang River estuary. The different distribution of the two species is interpreted as evidence of their distinct bloom ecology. Expanded efforts implementing these assays offer the ability to discriminate the dynamics of A. fundyense and A. pacificum blooms and provide a more sound basis for monitoring toxic Alexandrium species in this region. [ABSTRACT FROM AUTHOR]

Since 1990s, harmful algal blooms (HABs) of Kareniaceae, primarily caused by species of Karenia and Karlodinium and rarely by Takayama species, have been substantially increasing in frequency and duration in the coastal waters of China. In this study, we recorded a bloom of high abundance of T. acrotrocha in the Haizhou Bay, the Yellow Sea in September 2020, which is the first record of a Takayama bloom in the temperate coastal waters of China. We found that high concentrations of DON and DOP accelerated the proliferation of T. acrotrocha in the Haizhou Bay. Intensive mariculture, and terrestrial nitrogen and phosphorus input may be responsible for the eutrophication in the Haizhou Bay featuring high concentrations of DON and DOP, and high DIN/DIP ratios. The results suggested that, under ocean warming, the HABs of Kareniaceae are becoming increasingly dominant in eutrophic temperate coasts with intensive mariculture activities. • Takayama acrotrocha bloom was firstly recorded in Haizhou Bay, China's temperate coastal bay. • High organic nitrogen and phosphorus proliferated T. acrotrocha boom in Haizhou Bay. • Intensive mariculture activities and input of terrestrial nutrients eutrophicated Haizhou Gulf. [ABSTRACT FROM AUTHOR]

The Yellow Sea (YS) has been subjected to harmful algal blooms (HABs) for several decades. In this study, we compiled and analyzed a dataset of 165 red tides from 1972 to 2017 and a dataset of green tides from 2008 to 2017 in the YS. The most notable feature of red tides in the YS is the increasing dominance of dinoflagellate red tides in terms of frequency, scale, seasonal distribution, spatial coverage, and red tide causative species. The increasing dominance of dinoflagellate red tides is closely related to eutrophication and the development of the mariculture industry in the YS. However, the dinoflagellate red tides in the northern Yellow Sea (NYS) and the southern Yellow Sea (SYS) have different features. The apparent changes in red tides in the SYS in terms of frequency and seasonal patterns might have been caused by recurrent large-scale green tides in the last decade. • Datasets of red tides and green tides recorded in Yellow Sea (YS) were analyzed. • Dominance of dinoflagellate red tides increased in YS after 2000. • Dinoflagellate red tides in northern and southern YS exhibited different features. • Dinoflagellate red tides in YS were related to mariculture and eutrophication. • Red tides in southern YS might be affected by recurrent green tides. [ABSTRACT FROM AUTHOR]

The coastal waters adjacent to the Changjiang River estuary (CRE) are characterized by nutrient pollution and recurrent harmful algal blooms. In this study, resting cysts of Alexandrium pacificum Litaker and A. catenella (Whedon & Kof.) Balech, two major species within the A. tamarense species complex in Chinese coastal waters, were studied using sediment samples collected from the area adjacent to the CRE in May 2014 and December 2015. Cysts were detected with two real-time quantitative PCR assays, as well as the primuline-staining method. Only cysts of A. pacificum were found in the study area, which mainly distributed in the mud depositional zone near the CRE. A low-abundance region of the cysts present in spring is in accordance with the intrusive pathway of the Nearshore Kuroshio Branch Current (NKBC), suggesting that A. pacificum blooms could be regulated by seasonal intrusion of NKBC. • Alexandrium pacificum cysts in the East China Sea (ECS) were studied by qPCR assay. • A. pacificum cysts were mainly found in the mud zone near Changjiang River estuary. • Cyst distribution is affected by a Kuroshio Branch Current, NKBC, in spring. • Dynamics of A. pacificum bloom will be regulated by inter-annual variation of NKBC. [ABSTRACT FROM AUTHOR]

The Changjiang (Yangtze River) River estuary (CRE) and its adjacent coastal waters is a notable region for nutrient pollution, which results in severe problems of coastal eutrophication and harmful algal blooms (HABs). The occurrence of HABs, particularly those of dinoflagellate Alexandrium spp. capable of producing paralytic shellfish toxins (PSTs), has an increasing risk of contaminating seafood and poisoning human-beings. The investigation of PSTs, however, is often hampered by the relatively low abundance of Alexandrium spp. present in seawater. In this study, a monitoring strategy of PSTs using net-concentrated phytoplankton from a large volume of seawater was employed to examine spatiotemporal variations of PSTs in the CRE and its adjacent waters every month from February to September in 2015. Toxins in concentrated phytoplankton samples were analyzed using high-performance liquid chromatography coupled with a fluorescence detector (HPLC-FLD). The results showed that PSTs could be detected in phytoplankton samples during the sampling stage in the CRE and its adjacent waters. Toxin content increased gradually from February to May, reached the peak in June, and then decreased rapidly from July to September. The maximum value of PST content was 215 nmol m−3 in June. Low-potency toxins N -sulfocarbamoyl toxins 1/2 (C1/2) were the most dominant components of PST in phytoplankton samples from February to June in 2015, while high-potency gonyautoxin 4 (GTX4) became the dominant component from July to September. Toxins were mainly detected from three regions, the sea area north to the CRE, the sea area east to the CRE, and sea area near Zhoushan Island south to the CRE. Based on the results of this study, it can be inferred that the three regions around the CRE in May and June is of high risk for PST contamination and seafood poisoning. Image 1 • A strategy on toxin study using concentrated phytoplankton applied in East China Sea. • Eleven paralytic shellfish toxins were detected with HPLC during the sampling season. • N -sulfocarbamoyl toxin 1/2 were dominant before June and gonyautoxins 4 after July. • Three regions with high level toxins were identified around Changjiang River estuary. • Toxin content exhibited unimodal variation with high level toxins in May and June. Paralytic shellfish toxins (PSTs) in the sea area adjacent to the Changjiang River estuary were investigated to reveal the spatiotemporal variation patterns of PSTs. [ABSTRACT FROM AUTHOR]