The aim of this study was to characterize the relation of 5-hydroxytryptamine transporter(5-HTT) and the exercise-induced central fatigue. For the selection of the subjects, the blood was collected from male students attending a college, majoring in general studies (111 students) and pertinent to physical education (162 students), total 273 students, and 5-HTT genotype (SS type, LS type and LL type), was examined. Among them, 40 subjects suitable to the purpose of our study were selected at the end. The selected subjects were classified into 3 groups (16 cases in the SS type group, 15 cases in the LS type group, 9 cases in the LL type group), fatigue was induced by performing a long-term exercise (first VT intensity, second VO2max intensity, third VT intensity = average 58 minutes of exercise period), and the change of blood FFA, f-Trp, BCAAs, and f-Trp/BCAAs according to the 5-HTT genotype was examined, and the following results were obtained. In the FFA case, regarding the SS type, in comparison with the rest period, 30 minutes after the termination of exercise (p<.01), and in the comparison of immediately after the termination of exercise and 30 minutes after the termination of exercise, FFA increased significantly (p<.05), and after 24 hours, in comparison with 30 minutes after the termination, it decreased significantly (p<.05). The LS type, in comparison with the rest period, 30 minutes after the termination of exercise increased significantly (p<.05). On the other hand, the LL type, in comparison with the rest period, 30 minutes after the termination of exercise showed a significant increase (p<.05), and after 24 hours showed a significant decrease in comparison with 30 minutes after the termination of the exercise (p<.05). In regard to f-Trp, during the entire period, all three groups showed a similar trend, and in the LS type only, in comparison with immediately after the exercise, after 24 hours it significantly decreased (p<.05). In regard to BCAAs, in the SS type and the LS type, excluding the LL type, 30 minutes after the termination of exercise, significantly decreased in comparison with the rest period and immediately after exercise (p<.01, p<.01). Concerning the ratio of f-Trp and BCAAs (f-Trp/BCAAs), in all three groups, their trend according to the time point was similar, and only in the LS type, comparing after 24 hours with 30 minutes after the termination of exercise, it significantly decreased (p<.05). The reduction of plasma f-Trp/BCAAs after 24 hours was shown to be due to the elevation of BCAAs and the reduction of f-Trp. In the distribution of the frequency of genotype and alleles (n=273), it was shown that the SS type was 62.7% (n=171), the LS type was 30.0% (n=82), the LL type was 7.3% (n=20), the S allele was 77.7% (n=424), and the L allele was 22.3% (n=122). In other words, the LL type showed a very low frequency in comparison with the SS type and the LS type. Based on the above results, although a significant difference among different genotype groups was not detected, the concentration of f-Trp and the ratio of f-Trp/BCAAs in the LL type were detected to be higher than the SS type and the LS type. This suggests indirectly that during rest and the long term high intensity exercise. 5-HT synthesis and release were elevated. In other words, 5-HT neurotransmission may be higher in the LL type. Nonetheless, a significant difference among the groups according to genotypes was not detected, and thus it is difficult to generalize such results, however, during the entire blood collection period, the SS type and the LS type showed a trend lower that the LL type, hence it may be considered that, particularly, atheletes who repeat continuously a long term high intensity training, the SS type and the LS type of 5-HTT may act advantageously. [ABSTRACT FROM AUTHOR]