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OBJECTIVE: The purpose of this study was to uncover the regulatory effect of LINC00887 on the progression of nasopharyngeal carcinoma (NPC) and the underlying mechanism. PATIENTS AND METHODS: Relative level of LINC00887 in NPC tissues and cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Thereafter, the regulatory effect of LINC00887 on proliferative ability in SUNE-1 and HK-1 cells was examined by cell counting kit-8 (CCK-8) and 5-Ethynyl-2'-deoxyuridine (EdU) assay. Through Dual-Luciferase reporter gene assay and RNA-Binding Protein Immunoprecipitation (RIP) assay, the interaction in the regulatory loop LINC00887/miRNA-203b-3p/NUP205 was ascertained. At last, rescue experiments were conducted to clarify the involvement of the regulatory loop LINC00887/miRNA- 203b-3p/NUP205 in the progression of NPC. RESULTS: Results showed that LINC00887 was upregulated in NPC tissues and cells, and its overexpression markedly stimulated the proliferative ability in NPC cells. In addition, a potential interaction in the regulatory loop LINC00887/ miRNA-203b-3p/NUP205 was discovered, which was responsible for promoting the proliferative ability in NPC. CONCLUSIONS: LINC00887 promotes the proliferative ability in NPC via absorbing miRNA- 203b-3p to upregulate NUP205. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To explore the correlation of the suppressor of cytokine signaling 3 (SOCS3) gene polymorphism with childhood asthma. PATIENTS AND METHODS: A total of 204 asthma children (observation group) and 235 healthy children (control group) were enrolled. General clinical information of enrolled subjects was collected. Inflammatory factors and pulmonary function test indexes in each subject were examined. Moreover, the polymorphism of SOCS3 gene rs9914220 was detected with the TaqMan-MGB probe. RESULTS: Asthma children in the observation group exhibited higher levels of interleukin-4 (IL-4), IL-17, and IL-33 than those of the control group (p<0.05). The forced expiratory volume in 1 second (FEV1), FEV1/forced vital capacity (FVC) ratio (%), peak expiratory flow (PEF), and FVC in the observation group were lower than those in the control group. However, the residual volume (RV), and RV/total lung capacity (TLC) ratio were higher in observation group than those in control group (p<0.05). Distribution frequency of the genotypes varied a lot between the two groups (p<0.05). However, we did not observe a significant difference in SOCS3 alleles between the two groups (p>0.05). According to the analysis of the genetic model, there were differences in dominant and cumulative models between the two groups (p<0.05), whereas the recessive model was not different between the two groups (p>0.05). CONCLUSIONS: Levels of inflammatory factors and pulmonary functions help to effectively monitor the progression of childhood asthma, thus increasing the clinical diagnosis rate. The polymorphism of the SOCS3 gene rs9914220 site is correlated with the onset of childhood asthma. [ABSTRACT FROM AUTHOR]