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• The important points of our article " Antimicrobial Resistance and Extended-Spectrum Beta-Lactamase (ESBL) Genes in E. coli Isolated from Equine Fecal Samples in Turkey" by Akın Yiğin are stated below. • According to the data in the "Antibiotic Consumption Surveillance Report" published by the WHO in 2018, Turkey ranks first in the use of antibiotics as per defined daily dose per 1,000 people (as a percentage in total). • Turkey's horse breeding is very important, especially in eastern regions lifestyle. But until now, Expanded Spectrum Beta-Lactamase (ESBL) genes, such as blaCTX-M, blaTEM, blaSHV, blaOXA-48 and blaKPC, have never been studied by Realtime PCR in racehorses in this region. The results of this article are the first and most important study to highlight the case of antibiotic use and warn the regional authorities on this issue. • The study of ESBL genes in these combinations (blaCTX-M+blaSHV,blaCTX-M+blaTEM, blaSHV+ blaOXA-48, blaTEM+blaSHV, blaCTX+blaTEM+blaKPC, blaCTX-M+blaTEM+blaOXA-48, blaCTX-M+blaTEM+blaSHV, blaTEM+blaSHV+ blaKPC, blaCTX+blaSHV+ blaOXA-48, in blaCTX-M+blaTEM+blaSHV+blaOXA-48, blaCTX-M+blaTEM+ blaSHV+ blaKPC, blaCTX-M+blaTEM+blaSHV+ blaOXA-48+blaKPC) has increased the spesifity of the article. ESBL genes and their used in these combinations give us very detailed information primarily in the evaluation of the results. The extensive use of antibacterial agents used for treatment in human and veterinary clinics to increase yield in livestock and aquaculture in developing countries causes the detection and spread of multidrug-resistant (MDR) strains in different sources. The presence of Escherichia coli strains is very common in racehorses and it's a serious problem on horse farms. Extended-spectrum beta-lactamase (ESBL) production is an important source of MDR development in Gram-negative strains such as E.coli. This study aimed to detect the presence of ESBL genes in E. coli strains isolated from horse farms in eastern Turkey. A total of 200 equine fecal samples were collected from 16 horse farms (70 Thoroughbred and 130 Arabian horses) in the Adana and Şanlıurfa provinces of Turkey. The presence of ESBL genes, such as blaCTX-M, blaTEM, blaSHV, blaOXA-48, and blaKPC in the E. coli strains was investigated using real-time PCR. According to the real-time PCR results, 107 (53.5%) out of 200 E. coli strains were positive for at least one gene. BlaCTX-M, blaSHV, and blaTEM were detected in 11, 6, and 4 strains respectively. blaCTX-M+blaSHV coexisted in 16 strains, blaCTX-M+blaTEM in 11 strains, blaSHV+blaOXA-48 in 8 strains, blaTEM+blaSHV in 8 strains, blaCTX+blaSHV+ blaOXA-48 coexistent in 3 strains, blaCTX-M+blaTEM+blaSHV+blaOXA-48 in 7 strains, blaCTX-M+ blaTEM+blaSHV+blaKPC in 2 strains, and finally, blaCTX-M+blaTEM+blaSHV+blaOXA-48+blaKPC in 3 strains. In a summary, ESBL-producing E. coli strains were frequently seen in the racehorses from eastern Turkey. The excessive misuse of antibiotics has led to the evolution of MDR strains such as ESBL-producing E. coli that cause serious health problems and are difficult to treat. Routine molecular epidemiology studies are warranted to closely monitor these resistant strains. [ABSTRACT FROM AUTHOR]