28 results on '"Wu,Wen-Chuan"'
Search Results
2. Generation and directional decomposition of guided waves for finite-range defect detection in rail tracks
- Author
-
Wu, Wen-Chuan and Yin, Ching-Chung
- Abstract
Railway tracks exhibit a complex, sudden alteration in their cross-sectional configuration. Generating guided waves within thick rail tracks using conventional guided wave transducers is challenging. This research employs directional decomposition to comprehend how guided waves form within the rail. These waves arise from constructive interference between multiple reflected bulk waves, which are induced by point forces on the top surface of the railhead or diffracted from cracks in the rail structure. This study simulates finite-range detection of rail defects using finite element analysis to demonstrate a potential application to the guided wave rail inspection car. The transmitter and a series of monitoring points are located on the same side of the rail defect in the pitch-catch configuration. The transmitting guided waves often hide the small backscatter waves induced by defects. A directional filter extracts the small backscatter signals from the entire received data. A 100 kHz tone burst actuates on the top surface of the rail in three orthogonal directions. Numerical findings demonstrate that vertical and tangential excitations are suitable for detecting defects in the railhead and lower region, while transverse excitation and detection are appropriate for identifying defects in the rail web. The application of directional decomposition provides valuable insights into the complex process of backscatter waves arising from rail track defects.
- Published
- 2023
- Full Text
- View/download PDF
3. Nonlinear ultrasonic characterization of joint structures using backscatter guided waves
- Author
-
Wu, Wen-Chuan and Yin, Ching-Chung
- Abstract
Joint structures made from plates or shells are frequently used in large constructions, such as oil storage tanks in petrochemical industries. The corrosion-induced metal loss often appears on the back side of the bottom plate supported by ground or sand. The welding residual stress and weight of shell walls accelerate the corrosion. Determining the severity of metal loss under the joint is challenging. This work uses the mode pair technique for nondestructive inspection of inaccessible regions in joint structures. A finite element analysis model simulates the transient responses of the incident and mode-converted fundamental and second harmonic plate waves. The second harmonic s0 plate wave satisfying the phase velocity matching condition accompanies the transmitted fundamental SH0 plate wave. A directional filter identifies every mode-converted plate wave by comparing the frequency spectra with the dispersion curves. Linear ultrasonic guided waves can detect inaccessible regions using mode-converted reflections. The symmetry of the linear mode-converted fundamental plate waves depends on the defect position and geometry, not the size. The mode-converted second harmonic guided wave can achieve better spatial resolution for defect sizing. The nonlinear parameter shows a proportional trend with an increasing thickness reduction. Experimental and numerical evidence reveals nonlinear ultrasonic guided wave technique has the potential for defect detection in joint structures.
- Published
- 2023
- Full Text
- View/download PDF
4. Spectral-domain optical coherence tomography finding of acute retinal pigment epitheliitis.
- Author
-
Li, Yi-Hsuan, Chang, Yo-Chen, and Wu, Wen-Chuan
- Abstract
Acute retinal pigment epitheliitis (ARPE) is a rare, transient macular disorder affecting healthy young adults. We describe the morphologic appearance of the retina by spectral-domain optical coherence tomography (SD-OCT) and to evaluate both the anatomic changes and the functional visual acuity changes over time in the course of disease. A 35-year-old healthy male presented with 1-week history of sudden-onset bilateral central scotoma with blurry vision. He denied trauma, excessive sun exposure, or drug abuse history or alkyl nitrites before. The medical and ocular examinations were unremarkable. The best-corrected visual acuity (BCVA) was 20/200 (OU) at the initial visit. Slit-lamp examination result was normal. Fundus examination revealed subfoveal yellowish lesions with a halo-like pigment in both eyes. The SD-OCT imaging showed subtle disruption of the retinal pigment epithelium (RPE) and abnormal hyperreflectivity throughout the full thickness of the foveola in both eyes. Six weeks later, the BCVA improved to 20/30 (OU) without any treatment. Six months later, the BCVA observed deteriorated to 20/50 (OU). SD-OCT demonstrated ellipsoid zone and cone outer segment tips line defects at the fovea with sharply defined borders. One year later from the initial visit, the BCVA improved to 20/20 (OU), but persisted macular microhole presents on the SD-OCT. The patient was followed for 1 year without any treatment. Thereafter, we noted that in the case of poor initial visual acuity, external limiting membrane, or outer nuclear layer involvement, as determined by SD-OCT, at the baseline might need longer time for visual acuity. The natural course of ARPE may involve the demonstration of a minor outer retinal defect that is similar to a macular microhole. In ARPE, like SD-OCT findings, the location of the initial lesion is the photoreceptor outer segments. It is not just limited to the RPE. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
5. All-trans retinoic acid suppresses the adhering ability of ARPE-19 cells via mitogen-activated protein kinase and focal adhesion kinase
- Author
-
Chang, Yo-Chen, Chang, Yuh-Shin, Hsieh, Ming-Chu, Wu, Horng-Jiun, Wu, Meng-Hsien, Lin, Chia-Wei, Wu, Wen-Chuan, and Kao, Ying-Hsien
- Abstract
This study investigated the signaling mechanism underlying the anti-adhesive effect of all-trans retinoic acid (ATRA) on retinal pigment epithelial ARPE-19 cells. Adhesion kinetics with or without ATRA treatment were profiled by adhesion assay. Surface coating with type IV collagen, fibronectin, laminin, but not type I collagen, significantly enhanced adhesion and spreading of ARPE-19 cells, while ATRA at subtoxic doses (ranging from 10–7to 10–6 M) profoundly suppressed the extracellular matrix-enhanced adhesion ability. Cell attachment on FN activated PI3K/Akt and MAPK cascades, whereas ATRA pretreatment blunted the early phosphorylation of Akt and MAPK signaling mediators including p38 MAPK, JNK1/2, and ERK1/2. Mechanistically, signaling blockade with selective kinase inhibitors demonstrated that all MAPK pathways were involved in the anti-adhesive effect of ATRA, whereas the PI3K inhibitor treatment significantly potentiated the ATRA-suppressed RPE cell adhesion. Moreover, ATRA treatment did not affect intracellular F-actin distribution, but remarkably reduced focal adhesion kinase (FAK) expression and its nuclear localization during ARPE-19 cell attachment. In conclusion, ATRA suppresses the adhering ability of ARPE-19 cells at least in part through MAPK and FAK pathways. Signaling blockade with PI3K inhibitor could be regarded as an alternative modality for treating proliferative vitreoretinopathy.
- Published
- 2016
- Full Text
- View/download PDF
6. Image Retrieval Using Spatial Color and Edge Detection.
- Author
-
Ho, Yo-Sung, Kim, Hyoung Joong, Chang, Chin-Chen, Chou, Yung-Chen, and Wu, Wen-Chuan
- Abstract
To improve the effectiveness and efficiency of CBIR systems, in this paper, we present a novel IR scheme with multiple features, the spatial color and edge percentage features, derived by way of moment-preserving edge detection. Put the above two features together, we come by an effective and efficient IR system. Experimental results show that the proposed method outperforms other similar methods in terms of accuracy and retrieval efficiency. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
7. Using optical coherence tomography to evaluate macular changes after surgical management for rhegmatogenous retinal detachment
- Author
-
Cheng, Kai‐Chun, Cheng, Kai‐Yuan, Cheng, Kai‐Hung, Chen, Kuo‐Jen, Chen, Chia‐Hui, and Wu, Wen‐Chuan
- Abstract
Incomplete visual recovery, color vision defects, or persistent metamorphopsia may persist even after successful surgery for rhegmatogenous retinal detachment (RRD), especially in cases of RD with macula off, suggesting microstructural macular damage that standard fundus biomicroscopy could not detect. We compared spectral‐domain optical coherence tomography imaging with preoperative and postoperative visual acuity to evaluate the relationship between morphological changes in the outer retina and visual outcome after successful repair of RRD with macula on or off. We enrolled 43 patients (43 eyes) with successful repair of RRD and a minimum 6‐month follow up after surgery in this retrospective research. Patients accepted spectral‐domain optical coherence tomography postoperatively and visual acuity examination preoperatively and postoperatively. The mean age of the patients was 48.74 ± 12.68 years (range: 16–77 years). The mean visual acuity (logarithm of minimal angle of resolution) before surgery was 0.87 ± 0.70. Disrupted ellipsoid zone was noted in one of 11 eyes in the macula‐on group (9.1%) and 19 of 32 eyes in the macula‐off group (59.4%). Disrupted external limiting membrane (ELM) was noted in no eye in the macula‐on group (0%) and 11 of 32 eyes in the macula‐off group (34.4%). The macula‐off group was associated with better postoperative visual gains than the macula‐on group (p= 0.013). Patients with integrity of the ellipsoid zone and ELM were associated with significant visual improvement than patients with disruption of the ellipsoid zone or ELM.
- Published
- 2016
- Full Text
- View/download PDF
8. Using optical coherence tomography to evaluate macular changes after surgical management for rhegmatogenous retinal detachment
- Author
-
Cheng, Kai-Chun, Cheng, Kai-Yuan, Cheng, Kai-Hung, Chen, Kuo-Jen, Chen, Chia-Hui, and Wu, Wen-Chuan
- Abstract
Incomplete visual recovery, color vision defects, or persistent metamorphopsia may persist even after successful surgery for rhegmatogenous retinal detachment (RRD), especially in cases of RD with macula off, suggesting microstructural macular damage that standard fundus biomicroscopy could not detect. We compared spectral-domain optical coherence tomography imaging with preoperative and postoperative visual acuity to evaluate the relationship between morphological changes in the outer retina and visual outcome after successful repair of RRD with macula on or off. We enrolled 43 patients (43 eyes) with successful repair of RRD and a minimum 6-month follow up after surgery in this retrospective research. Patients accepted spectral-domain optical coherence tomography postoperatively and visual acuity examination preoperatively and postoperatively. The mean age of the patients was 48.74 ± 12.68 years (range: 16–77 years). The mean visual acuity (logarithm of minimal angle of resolution) before surgery was 0.87 ± 0.70. Disrupted ellipsoid zone was noted in one of 11 eyes in the macula-on group (9.1%) and 19 of 32 eyes in the macula-off group (59.4%). Disrupted external limiting membrane (ELM) was noted in no eye in the macula-on group (0%) and 11 of 32 eyes in the macula-off group (34.4%). The macula-off group was associated with better postoperative visual gains than the macula-on group (p = 0.013). Patients with integrity of the ellipsoid zone and ELM were associated with significant visual improvement than patients with disruption of the ellipsoid zone or ELM.
- Published
- 2016
- Full Text
- View/download PDF
9. The visual status of children ages 3 to 6 years in the vision screening program in Taiwan.
- Author
-
Lai, Yu-Hung, Hsu, Hsin-Tien, Wang, Hwei-Zu, Chang, Shun-Jen, and Wu, Wen-Chuan
- Subjects
VISION testing ,VISION disorders ,CHILD health services - Abstract
Purpose: To investigate the current status of myopia, amblyopia, and strabismus of preschoolers in Taiwan. Methods: The records of a vision screening performed in 2005 at 4 preschools by a tertiary referral medical center in Kaohsiung, Taiwan were reviewed. Cycloplegic refraction, visual acuity by E chart, stereopsis by Titmus and National Taiwan University tests, and ocular alignment were analyzed. Results: Most (97.7%) of the children (n = 618) were able to perform visual acuity testing. There was good correlation between the results of cycloplegic spherical equivalent obtained by autorefraction and by retinoscopy (r = 0.934, p < 0.001). The prevalence of amblyopia was about 5%. A trend was found between amblyopia morbidity and the 4 age groups when a single criterion (best-corrected visual acuity <0.7) was used for all ages (decreased amblyopia incidence with increased age; p = 0.028, χ
2 test for trend). The prevalence of myopia was 3.0%, 4.2%, 4.7%, and 12.2% at ages 3, 4, 5, and 6 years, respectively. There was a trend of increased myopia with increased age (p = 0.021, χ2 test for trend). Conclusions: The prevalence of myopia in children ages 3 to 6 years in Taiwan was high compared with the prevalence in other countries. Criteria for amblyopia based on age difference and on the types of visual acuity test are recommended.▪ [Copyright &y& Elsevier]- Published
- 2009
- Full Text
- View/download PDF
10. Prognosis of Taiwanese patients with isolated optic neuritis after intravenous methylprednisolone pulse therapy.
- Author
-
Chang, Yo-Chen, Wu, Wen-Chuan, and Tsai, Rong-Kung
- Subjects
OPTIC neuritis ,INTRAVENOUS therapy ,PATIENTS ,CHRONIC diseases ,DRUG administration ,INTRAVENOUS injections ,MULTIPLE sclerosis ,PROGNOSIS ,DISEASE relapse ,RETROSPECTIVE studies ,METHYLPREDNISOLONE ,DISEASE complications - Abstract
Background/purpose: To evaluate the clinical manifestations, prognosis, recurrence rate and development of multiple sclerosis between papillitis group and retrobulbar group in Taiwanese patients with isolated acute optic neuritis (AON) after treatment with intravenous methylprednisolone pulse therapy.Methods: We retrospectively reviewed patients with AON who had received intravenous methylprednisolone pulse therapy. These patients were classified into retrobulbar or papillitis groups. Demographic characteristics, responsiveness to pulse therapy, recurrence rate and incidence of multiple sclerosis were compared between these two groups.Results: Of the 43 patients enrolled in this study, 19 patients (44%) were in the retrobulbar group and 24 patients (56%) were in the papillitis group. Seven cases (16%) showed periventricular plaque on magnetic resonance imaging (MRI). Among these seven patients, five developed definite or probable multiple sclerosis. The incidence of multiple sclerosis in patients with positive brain MRI findings was significantly higher than in patients with negative MRI findings (p = 0.002). There was no statistical difference in final visual acuity between the two group (p = 0.353). Sixteen patients suffered from recurrence of AON (21% in the papillitis group and 58% in the retrobulbar group, p = 0.029). Two patients (8%) in the papillitis group and six patients (32%) in the retrobulbar group developed multiple sclerosis (p = 0.061) with a mean interval of 21.6 +/- 11.2 months.Conclusion: AON in Taiwan has a relatively lower percentage of development of multiple sclerosis than in Western countries. The presence of periventricular plaque on MRI is significantly associated with the later development of multiple sclerosis. The retrobulbar group had a stronger association with recurrence and development of multiple sclerosis. [ABSTRACT FROM AUTHOR]- Published
- 2007
- Full Text
- View/download PDF
11. Pharmacological implications from the adhesion‐induced signaling profiles in cultured human retinal pigment epithelial cells
- Author
-
Wu, Wen‐Chuan, Chang, Yo‐Chen, Wu, Kwou‐Yeung, Chen, Su‐Yueh, Hsieh, Ming‐Chu, Wu, Meng‐Hsien, Wu, Horng‐Jiun, Wu, Wen‐Sheng, and Kao, Ying‐Hsien
- Abstract
Extracellular matrix (ECM) plays an active and complex role in regulating cellular behaviors, including proliferation and adhesion. This study aimed at delineating the adhesion‐induced signaling profiles in cultured human retinal pigment epithelium (RPE) cells and investigating the antiadhesion effect of antiproliferative drugs in this context. RPE R‐50 cells grown on various ECM molecules, such as type I and IV collagens, fibronectin, and laminin, were used for adhesion assay and for examining the phosphorylation profiles of signaling mediators including Akt, extracellular signal‐regulated kinase (ERK) 1/2, and integrin‐linked kinase (ILK) using Western blotting. The cells receiving antiproliferative drug treatment at subtoxic doses were used to evaluate their antiadhesive and suppressive effects on kinase activities. ECM coating enhanced adhesion and spreading of RPE cells significantly. The cellular attachment onto ECM‐coated surfaces differentially induced Akt, ERK1/2, and ILK phosphorylation, and concomitantly increased p53 phosphorylation and cyclin D1 expression, but decreased Bcl‐2/Bax ratios. Treatment with antiproliferative agents, including 5‐fluorouracil, mitomycin C, and daunomycin, at subtoxic doses suppressed the ability of RPE cells to adhere to ECM substratum significantly. This suppression was in part mediated through reduction of integrin β1 and β3 expressions and interfering Akt‐ILK signaling activity. Mechanistically, blockade of PI3K/Akt signaling resulted in the suppressed adhesion of RPE cells to ECM. These findings support the hypothesis that, in addition to their antimitogenic effect, antiproliferative agents also exhibit suppressive effect on the adhesiveness of cultured RPE cells. Moreover, inhibitors of the PI3K/Akt signaling mediator can potentially be used as therapeutic agents for proliferative vitreoretinopathy.
- Published
- 2014
- Full Text
- View/download PDF
12. Pharmacological implications from the adhesion-induced signaling profiles in cultured human retinal pigment epithelial cells
- Author
-
Wu, Wen-Chuan, Chang, Yo-Chen, Wu, Kwou-Yeung, Chen, Su-Yueh, Hsieh, Ming-Chu, Wu, Meng-Hsien, Wu, Horng-Jiun, Wu, Wen-Sheng, and Kao, Ying-Hsien
- Abstract
Extracellular matrix (ECM) plays an active and complex role in regulating cellular behaviors, including proliferation and adhesion. This study aimed at delineating the adhesion-induced signaling profiles in cultured human retinal pigment epithelium (RPE) cells and investigating the antiadhesion effect of antiproliferative drugs in this context. RPE R-50 cells grown on various ECM molecules, such as type I and IV collagens, fibronectin, and laminin, were used for adhesion assay and for examining the phosphorylation profiles of signaling mediators including Akt, extracellular signal-regulated kinase (ERK) 1/2, and integrin-linked kinase (ILK) using Western blotting. The cells receiving antiproliferative drug treatment at subtoxic doses were used to evaluate their antiadhesive and suppressive effects on kinase activities. ECM coating enhanced adhesion and spreading of RPE cells significantly. The cellular attachment onto ECM-coated surfaces differentially induced Akt, ERK1/2, and ILK phosphorylation, and concomitantly increased p53 phosphorylation and cyclin D1 expression, but decreased Bcl-2/Bax ratios. Treatment with antiproliferative agents, including 5-fluorouracil, mitomycin C, and daunomycin, at subtoxic doses suppressed the ability of RPE cells to adhere to ECM substratum significantly. This suppression was in part mediated through reduction of integrin β1 and β3 expressions and interfering Akt-ILK signaling activity. Mechanistically, blockade of PI3K/Akt signaling resulted in the suppressed adhesion of RPE cells to ECM. These findings support the hypothesis that, in addition to their antimitogenic effect, antiproliferative agents also exhibit suppressive effect on the adhesiveness of cultured RPE cells. Moreover, inhibitors of the PI3K/Akt signaling mediator can potentially be used as therapeutic agents for proliferative vitreoretinopathy.
- Published
- 2014
- Full Text
- View/download PDF
13. Anatomic and functional outcome after surgical removal of idiopathic macular epiretinal membrane
- Author
-
Lee, Po‐Yen, Cheng, Kai‐Chun, and Wu, Wen‐Chuan
- Abstract
The purpose of this study is to investigate the functional and structural outcomes of surgical treatment of eyes with idiopathic macular epiretinal membrane (ERM). Clinical records of 21 patients (22 eyes) who underwent macular ERM removal with at least 6 months of postoperative follow‐up period were reviewed retrospectively. All patients were treated with pars plana vitrectomy and ERM peeling surgery. Fourteen patients also underwent cataract surgery at the same time. Pre‐ and postoperative visual acuity (VA), intraocular pressure (IOP), and macular thickness along with postoperative adverse events were all recorded. The mean follow‐up was 8.5 ± 3.2 months. Three eyes were pseudophakic and 19 eyes were phakic preoperatively. Five phakic eyes undergoing simple ERM peeling surgery had worsened cataracts, and vision was recovered after subsequent cataract extraction surgery. Mean preoperative IOP was 13.1 ± 4.2 mmHg, which did not differ significantly postoperatively (p= 0.228). One patient had increased IOP postoperatively and needed topical antiglaucoma treatment. Thirteen eyes showed visual improvement, and six eyes became worse during the follow‐up. Mean preoperative best‐corrected VA was 0.36 decimal equivalent, which was then converted to logarithm of the minimum angle of resolution (logMAR 0.502 ± 0.259) for statistical analysis; this had improved to a mean of 0.536 decimal equivalent (logMAR 0.38 ± 0.35) at the final follow‐up. Mean central macular thickness (CMT) also significantly declined (p< 0.0001). From an analysis of dividing patients into two subgroups according to clinical severity [macular pucker (MP) and cellophane maculopathy (CM)], measured mean CMT of both groups demonstrated significant reduction at final follow‐up (MP, p< 0.0001; CM, p= 0.005). Mean final best‐corrected VA also reached significant improvement in MP group (p= 0.008). However, in the CM group, no significant change in VA was observed (p= 0.52). Besides, VA measurements in the MP group had improved significantly to that of CM (p= 0.037). The measured CMTs of the MP group had also reduced significantly (p= 0.046) compared with those of the CM group. In conclusion, membrane peeling surgery can lead to a significant reduction of macular edema in patients with idiopathic macular ERM. The advantages of combined peeling and cataract surgery will achieve maximum positive visual outcome.
- Published
- 2011
- Full Text
- View/download PDF
14. Anatomic and functional outcome after surgical removal of idiopathic macular epiretinal membrane
- Author
-
Lee, Po-Yen, Cheng, Kai-Chun, and Wu, Wen-Chuan
- Abstract
The purpose of this study is to investigate the functional and structural outcomes of surgical treatment of eyes with idiopathic macular epiretinal membrane (ERM). Clinical records of 21 patients (22 eyes) who underwent macular ERM removal with at least 6 months of postoperative follow-up period were reviewed retrospectively. All patients were treated with pars plana vitrectomy and ERM peeling surgery. Fourteen patients also underwent cataract surgery at the same time. Pre- and postoperative visual acuity (VA), intraocular pressure (IOP), and macular thickness along with postoperative adverse events were all recorded. The mean follow-up was 8.5±3.2 months. Three eyes were pseudophakic and 19 eyes were phakic preoperatively. Five phakic eyes undergoing simple ERM peeling surgery had worsened cataracts, and vision was recovered after subsequent cataract extraction surgery. Mean preoperative IOP was 13.1±4.2mmHg, which did not differ significantly postoperatively (p=0.228). One patient had increased IOP postoperatively and needed topical antiglaucoma treatment. Thirteen eyes showed visual improvement, and six eyes became worse during the follow-up. Mean preoperative best-corrected VA was 0.36 decimal equivalent, which was then converted to logarithm of the minimum angle of resolution (logMAR 0.502±0.259) for statistical analysis; this had improved to a mean of 0.536 decimal equivalent (logMAR 0.38±0.35) at the final follow-up. Mean central macular thickness (CMT) also significantly declined (p<0.0001). From an analysis of dividing patients into two subgroups according to clinical severity [macular pucker (MP) and cellophane maculopathy (CM)], measured mean CMT of both groups demonstrated significant reduction at final follow-up (MP, p<0.0001; CM, p=0.005). Mean final best-corrected VA also reached significant improvement in MP group (p=0.008). However, in the CM group, no significant change in VA was observed (p=0.52). Besides, VA measurements in the MP group had improved significantly to that of CM (p=0.037). The measured CMTs of the MP group had also reduced significantly (p=0.046) compared with those of the CM group. In conclusion, membrane peeling surgery can lead to a significant reduction of macular edema in patients with idiopathic macular ERM. The advantages of combined peeling and cataract surgery will achieve maximum positive visual outcome.
- Published
- 2011
- Full Text
- View/download PDF
15. Clinical epidemiological analysis of the relationship between pancreatic cancer and diabetes mellitus: Data from a single institution in China
- Author
-
KUANG, Tian Tao, JIN, Da Yong, WANG, Dan Song, XU, Xue Feng, NI, Xiao Ling, WU, Wen Chuan, and LOU, Wen Hui
- Abstract
To investigate the relationship between pancreatic cancer (PC) and diabetes mellitus.
- Published
- 2009
- Full Text
- View/download PDF
16. Elevation of Intraocular Pressure After Intravitreal Injection of Triamcinolone Acetonide in Taiwanese Patients
- Author
-
Chang, Yo‐Chen and Wu, Wen‐Chuan
- Abstract
The purpose of this study was to investigate the incidence and timing of elevation in intraocular pressure (IOP) after intravitreal injection of triamcinolone acetonide (IVTA). This was a retrospective observational case series that included 52 eyes from 52 patients. All patients received intravitreal injection with 4 mg of triamcinolone acetonide. Significant IOP elevation was defined as pressure greater than 21 mmHg. There were 26 males and 26 females included in this study. The mean age was 59.4 years. The mean IOP at baseline and postoperative mean highest IOP were 14.6 mmHg and 21.5 mmHg, respectively. There were 19 (36.5%) patients who experienced significant IOP elevation at a mean of 26.0 days after IVTA. IOP was well‐controlled by topical antiglaucoma medication in 17 of these 19 patients. IOP elevation after intravitreal injection with 4 mg of triamcinolone acetonide is not a rare finding. This phenomenon usually starts approximately 1 month after IVTA. Patients considering this treatment should be fully informed of this known adverse effect.
- Published
- 2008
- Full Text
- View/download PDF
17. Elevation of Intraocular Pressure After Intravitreal Injection of Triamcinolone Acetonide in Taiwanese Patients
- Author
-
Chang, Yo-Chen and Wu, Wen-Chuan
- Abstract
The purpose of this study was to investigate the incidence and timing of elevation in intraocular pressure (IOP) after intravitreal injection of triamcinolone acetonide (IVTA). This was a retrospective observational case series that included 52 eyes from 52 patients. All patients received intravitreal injection with 4 mg of triamcinolone acetonide. Significant IOP elevation was defined as pressure greater than 21 mmHg. There were 26 males and 26 females included in this study. The mean age was 59.4 years. The mean IOP at baseline and postoperative mean highest IOP were 14.6 mmHg and 21.5 mmHg, respectively. There were 19 (36.5%) patients who experienced significant IOP elevation at a mean of 26.0 days after IVTA. IOP was well-controlled by topical antiglaucoma medication in 17 of these 19 patients. IOP elevation after intravitreal injection with 4 mg of triamcinolone acetonide is not a rare finding. This phenomenon usually starts approximately 1 month after IVTA. Patients considering this treatment should be fully informed of this known adverse effect.
- Published
- 2008
- Full Text
- View/download PDF
18. Comparison of Vitrectomy Alone and Combined Vitrectomy, Phacoemulsification and Intraocular Lens Implantation for Proliferative Diabetic Retinopathy
- Author
-
Tseng, Han‐Yi, Wu, Wen‐Chuan, and Hsu, Sheng‐Yao
- Abstract
There have been concerns that there may be an increased incidence of iris neovascularization (NV) following lens removal in patients with proliferative diabetic retinopathy (PDR). In this study, we retrospectively compared vitrectomy alone and vitrectomy combined with phacoemulsification (phacovitrectomy) and intraocular lens implantation regarding both complications and results. Fifty‐three eyes for vitrectomy group and 31 eyes for phacovitrectomy group were included. Postoperative iris and angle NV were found in eight (15.1%) eyes in the first group and no (0%) eyes in the second. The incidence was significantly lower (p< 0.05) in the phacovitrectomy group. The final vision gain of one or more lines was found in 17 (32.1%) and 21 (67.7%) eyes, respectively. There was significantly better vision improvement in the phacovitrectomy group. We consider the combined procedure to be useful as an alternative surgical treatment for patients with PDR and cataract formation.
- Published
- 2007
- Full Text
- View/download PDF
19. Comparison of Vitrectomy Alone and Combined Vitrectomy, Phacoemulsification and Intraocular Lens Implantation for Proliferative Diabetic Retinopathy
- Author
-
Tseng, Han-Yi, Wu, Wen-Chuan, and Hsu, Sheng-Yao
- Abstract
There have been concerns that there may be an increased incidence of iris neovascularization (NV) following lens removal in patients with proliferative diabetic retinopathy (PDR). In this study, we retrospectively compared vitrectomy alone and vitrectomy combined with phacoemulsification (phacovitrectomy) and intraocular lens implantation regarding both complications and results. Fifty-three eyes for vitrectomy group and 31 eyes for phacovitrectomy group were included. Postoperative iris and angle NV were found in eight (15.1%) eyes in the first group and no (0%) eyes in the second. The incidence was significantly lower ( p< 0.05) in the phacovitrectomy group. The final vision gain of one or more lines was found in 17 (32.1%) and 21 (67.7%) eyes, respectively. There was significantly better vision improvement in the phacovitrectomy group. We consider the combined procedure to be useful as an alternative surgical treatment for patients with PDR and cataract formation.
- Published
- 2007
- Full Text
- View/download PDF
20. Intravitreal Triamcinolone Acetonide for Patients with Macular Edema due to Branch Retinal Vein Occlusion
- Author
-
Cheng, Kai-Chun and Wu, Wen-Chuan
- Published
- 2006
- Full Text
- View/download PDF
21. Intravitreal Triamcinolone Acetonide for Patients with Macular Edema due to Branch Retinal Vein Occlusion
- Author
-
Cheng, Kai‐Chun and Wu, Wen‐Chuan
- Abstract
We designed a case series study to evaluate the outcome of intravitreal triamcinolone acetonide for the treatment of macular edema due to branch retinal vein occlusion (BRVO). The prospective comparative nonrandomized clinical interventional study included 27 patients (27 eyes) with macular edema due to BRVO. The study group consisted of 16 patients who had accepted an intravitreal injection (IVI) of 4 mg triamcinolone acetonide. The control group included 11 patients without IVI of triamcinolone acetonide. The mean follow‐up was 103.00 36.24 days in the study group and 94.55 36.31 days in the control group. In the study group, visual acuity measurements improved significantly (p0.001) from 0.77 0.43 logarithm of minimal angle of resolution (logMAR) preoperatively to a best postoperative visual acuity of 0.44 0.43 logMAR. Fourteen eyes (87.5%) gained improvement in visual acuity, with 10 eyes (62.5%) showing an increase in visual acuity of at least two Snellen lines. All 16 patients showed significant macular edema resolution in optical coherence tomography examination (p0.001) and perivascular leakage decrease in fluorescein angiography post‐IVI. In the control group, baseline best‐corrected visual acuity and best‐corrected visual acuity during the follow‐up did not vary significantly (p0.294). In conclusion, IVI of triamcinolone acetonide can lead to an increase in visual acuity and a resolution of macular edema in patients with BRVO.
- Published
- 2006
- Full Text
- View/download PDF
22. Culture of Retinal Pigment Epithelium from Evisceration Specimens
- Author
-
Tseng, Han‐Yi, Wu, Wen‐Chuan, Wu, Hong‐Jun, and Kao, Ying‐Hsien
- Abstract
Human retinal pigment epithelial (RPE) cell cultures are usually obtained from donor eyes; isolation and culture of RPE cells obtained by evisceration has not been reported previously. The present study attempted to isolate and cultivate RPE cells from evisceration specimens obtained from two cases with severe ocular trauma. Two different isolation methods, explantation and enzymatic dissociation, were used. In Case 1, RPE cells grew from the explants, but were contaminated with other cells such as fibroblasts and melanocytes, and no pure RPE cultures were obtained by explantation. In Case 2, RPE cells were separated from choroids using 0.25% trypsin before plating for culture, which effectively eliminated contaminating cells. A pure RPE culture was obtained and cultured with F12 medium supplemented with 30% fetal bovine serum. With this enzymatic dissociation method, cultured RPE cells grew to confluence in primary culture and could be maintained in culture for five passages. Cultured RPE cells were identified by the presence of cytokeratin expression, as shown by immunocytochemical staining. These isolation and culture methods provide alternative sources for human RPE cells and could be useful in studies of the cell biology and pathophysiology of human RPE cells.
- Published
- 2004
- Full Text
- View/download PDF
23. Culture of Retinal Pigment Epithelium from Evisceration Specimens
- Author
-
Tseng, Han-Yi, Wu, Wen-Chuan, Wu, Hong-Jun, and Kao, Ying-Hsien
- Published
- 2004
- Full Text
- View/download PDF
24. Transposable elements of Xanthomonas campestris pv. citri originating from indigenous plasmids
- Author
-
Tu, Jenn, Wang, Huei-Rong, Chang, Shau-Feng, Charng, Yuh-Chyang, Lurz, Rudi, Dobrinski, Beate, and Wu, Wen-Chuan
- Abstract
Summary: After introduction of the broad host range plasmid RP4 inXanthomonas campestris pv.citri strain XAS4501 twoXanthomonas transposable elements, ISXC4 and ISXC5, were isolated. These elements were found to be capable of transposition inEscherichia coli. Restriction analysis, DNA hybridization and heteroduplex experiments revealed that ISXC4 and ISXC5 are about 5.55 and 6.95 kb in size, respectively, possess inverted repeats about 50±18 bp in length and share DNA homology in their left (5.0 kb) and right (0.6 kb) ends. ISXC4 and ISXC5 were found to originate from plasmids pXW45N and pXW45J, which are indigenous replicons inX. campestris pv.citri strain XW45.
- Published
- 1989
- Full Text
- View/download PDF
25. Use of Dynamic Capillaroscopy for Studying Cutaneous Microcirculation in Patients with Diabetes Mellitus
- Author
-
Chang, Chung-Hsing, Tsai, Rong-Kung, Wu, Wen-Chuan, Kuo, Song-Ling, and Yu, Hsin-Su
- Abstract
[is0p9]To investigate the relationship between cutaneous microcirculation and retinopathy in diabetes mellitus, we studied by dynamic capillaroscopy 35 diabetes mellitus patients, including 10 patients without retinopathy, 10 patients with background retinopathy, and 15 patients with proliferative retinopathy. We found that (1) cutaneous and retinal microcirculation in diabetes mellitus in long-term diabetes mellitus, regressional rather than proliferational changes occurred in skin vasculature as compared to that in retina; (2) functional impairments of cutaneous microcirculation, that is, decreased resting capillary blood cell velocity, peak blood cell velocity, and prolonged time to peak capillary blood cell velocity by dynamic capillaroscopy, could be detected in diabetes mellitus patients without retinopathy; (3) the degree of tortouosity of capillaries, impairment in peak capillary blood cell velocity, and time to peak capillary blood cell velocity of cutaneous microcirculation were significantly correlated with the gravity of retinopathy in diabetes mellitus patients; (4) abnormal leakage of fluorescence in cutaneous microcirculation appeared in diabetes mellitus patients with or without abnormal fluorescein angiography in retina. In conclusion, dynamic capillaroscopy used in concert with ophthalmoscopy and fluorescein angiography can facilitate a comprehensive examination of vasculopathy in diabetes mellitus.
- Published
- 1997
- Full Text
- View/download PDF
26. Spontaneous separation of idiopathic epiretinal membrane after retinoid acid use
- Author
-
Lai, Hung‐Chi, Wu, Horng‐Jiun, and Wu, Wen‐Chuan
- Published
- 2019
- Full Text
- View/download PDF
27. MANAGEMENT OF PAPILLOPHLEBITIS ASSOCIATED CYSTOID MACULAR EDEMA WITH INTRAVITREAL TRIAMCINOLONE ACETONIDE A CASE REPORT
- Author
-
Chang, Yo-Chen and Wu, Wen-Chuan
- Published
- 2007
- Full Text
- View/download PDF
28. ITRAQ-based quantitative proteomics reveals apolipoprotein A-I and transferrin as potential serum markers in CA19-9 negative pancreatic ductal adenocarcinoma
- Author
-
Lin, Chao, Wu, Wen-Chuan, Zhao, Guo-Chao, Wang, Dan-Song, Lou, Wen-Hui, Jin, Da-Yong, and Bombardiere., Sergio Gonzalez
- Abstract
Supplemental Digital Content is available in the text
- Published
- 2016
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.