1. Surface Acoustic Wave (SAW)-Impedance Sensor for Kinetic Assay of Trypsin
- Author
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Cai, Qingyun, Wang, Ronghui, Wu, Liyin, Nie, Lihua, and Yao, Shouzhuo
- Abstract
The trypsin-catalyzed hydrolysis of benzoyl-l-arginine ethyl ester (BAEE) has been studied systematically at pH 7.8 and 32°C with a nonlinear regression model. The Michaelis constants covering the range from pH 7.0 to 8.6 were determined. The concentrations of enzyme isolated from pig pancreas were determined using the SAW-impedance sensor and the spectrophotometry, respectively. The experimental detection limit of trypsin was 0.3 mU/ml22mU/ml expresses milliunits per milliliter. One unit of enzyme is defined as the amount of trypsin required to hydrolyze 1 μmol of substrate per minute at 32°C and pH 7.8.and the recovery of the sensor system ranged from 94.7 to 105% (n= 6). The effects of temperature, pH, and some metal ions on the trypsin activity were investigated.
- Published
- 1997
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