26 results on '"Wiesneth, M"'
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2. Kommentar zur neuen „Richtlinie zur Herstellung und Anwendung von hämatopoetischen Stammzellzubereitungen“ vom 18. August 2014
- Author
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Wiesneth, M.
- Published
- 2015
- Full Text
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3. Transient Genetic Labeling of Human CD34-Positive Hematopoietic Stem Cells Using Nucleofection
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Greiner, J., Wiehe, J., Wiesneth, M., Zwaka, T.P., Prill, T., Schwarz, K., Bienek-Ziolkowski, M., Schmitt, M., Döhner, H., Hombach, V., and Torzewski, J.
- Abstract
Background:Human hematopoietic stem cells (HSCs) are used for autologous and allogeneic stem cell transplantation. Currently, HSCs serve as target cells for tissue engineering, gene transfer, and gene therapeutic approaches. However, viral transfection systems involve the risk of tumor induction by unspecific genomic integration, whereas non-viral transfection systems often fail due to low transfection efficiency. Material and Methods:CD34-positive peripheral blood stem cells (PBSCs) and bone marrow stem cells (BMSCs) were prepared by immunomagnetic selection with a purity > 99% for PBSCs and > 95% for BMSCs. Cells were transfected with the truncated low-affinity nerve growth factor receptor (deltaLNGFR) using the nucleofection technique. Transfection efficiency and kinetics were measured by FACS analysis, immunohistochemistry, immunofluorescence microscopy, and RT-PCR. Real-time PCR and Southern blot analysis were performed to detect the number of plasmids per cell during long-time cell culture and genomic integration of the transgene, respectively. Results:High transfection efficiency up to 45% for PBSCs and up to 36% for BMSCs was achieved without a marked decrease of cell viability. DeltaLNGFR expression decreased on proliferating HSCs with time, but the marker gene was significantly expressed over a 200-hour time period. No genomic integration of the transgene was observed. Conclusions:Nucleofection is a highly efficient, nontoxic method for transient genetic labeling of HSCs. Transient transfection of HSCs with the marker gene deltaLNGFR is rapid, specific and shows no relevant cell toxicity. This method is useful for in vitro protocols but also for in vivo marker studies in humans.
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- 2004
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4. Transient Genetic Labeling of Human CD34-Positive Hematopoietic Stem Cells Using Nucleofection
- Author
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Greiner, J., Wiehe, J., Wiesneth, M., Zwaka, T.P., Prill, T., Schwarz, K., Bienek-Ziolkowski, M., Schmitt, M., Döhner, H., Hombach, V., and Torzewski, J.
- Abstract
Summary Background: Human hematopoietic stem cells (HSCs) are used for autologous and allogeneic stem cell transplantation. Currently, HSCs serve as target cells for tissue engineering, gene transfer, and gene therapeutic approaches. However, viral transfection systems involve the risk of tumor induction by unspecific genomic integration, whereas non-viral transfection systems often fail due to low transfection efficiency. Material and Methods: CD34-positive peripheral blood stem cells (PBSCs) and bone marrow stem cells (BMSCs) were prepared by immunomagnetic selection with a purity > 99% for PBSCs and > 95% for BMSCs. Cells were transfected with the truncated low-affinity nerve growth factor receptor (deltaLNGFR) using the nucleofection technique. Transfection efficiency and kinetics were measured by FACS analysis, immunohistochemistry, immunofluorescence microscopy, and RT-PCR. Real-time PCR and Southern blot analysis were performed to detect the number of plasmids per cell during long-time cell culture and genomic integration of the transgene, respectively. Results: High transfection efficiency up to 45% for PBSCs and up to 36% for BMSCs was achieved without a marked decrease of cell viability. DeltaLNGFR expression decreased on proliferating HSCs with time, but the marker gene was significantly expressed over a 200-hour time period. No genomic integration of the transgene was observed. Conclusions: Nucleofection is a highly efficient, nontoxic method for transient genetic labeling of HSCs. Transient transfection of HSCs with the marker gene deltaLNGFR is rapid, specific and shows no relevant cell toxicity. This method is useful for in vitro protocols but also for in vivo marker studies in humans. Copyright © 2004 S. Karger GmbH, Freiburg- Published
- 2004
5. Functional State of Steroid- versus G-CSF-Mobilized Granulocytes: Considerations about the Storage of Granulocyte Concentrates for Neutropenic Patients
- Author
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Schmitt, A., Reinhardt, P., Schmitt, M., Nowak-Harnau, S., Maccari, B., Schulz, A., Kubanek, B., and Wiesneth, M.
- Abstract
Background: High yields of granulocytes mobilized with G-CSF sparked new enthusiasm for transfusion in patients with severe neutropenia. The in vitro and in vivo function of granulocytes prior to and following storage was investigated. Material and Methods: Granulocyte concentrates collected from 10 donors receiving prednisone or G-CSF before leukapheresis were evaluated over 48 h of storage for differential blood count, pH, electrolytes, viability, phagocytosis, oxidative burst, and secretion of IL-1β, IL-6, IL-8 and TNFα. The follow-up of 5 transfused patients was monitored by blood counts, Creactive protein, and clinical outcome. Results: Compared to fresh granulocyte concentrates, no major changes in viability, phagocytosis, oxidative burst, and cytokine profile were seen in granulocytes stored overnight. In contrast, significant differences were observed after 48 h of storage. Steroid- and G-CSF-mobilized granulocytes showed no major differences in the first 24 h, except for changes in pH and IL-8 secretion. Transfusions of G-CSF-mobilized granulocytes stored overnight were well tolerated without additional adverse effects. Conclusion: G-CSF increased the yield of leukocytes 3.5-fold without enhancing side effects after granulocyte transfusions. The in vitro and in vivo function of G-CSF-mobilized granulocytes remained stable during storage for 24 h. Thus, the therapeutic use of G-CSF-mobilized granulocyte concentrates is facilitated by the possibility of overnight storage.
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- 2002
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6. Granulocyte transfusions in children
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Pflieger, H., Wiesneth, M., Arnold, R., Niethammer, D., and Kleihauer, E.
- Abstract
Fifteen children with haematological diseases received an average of 10 granulocyte transfusions each, with 4.7×10
10 granulocytes per m2 body surface area (BSA) per transfusion. All patients except one had less than 100 granulocytes per µl blood combined with aplasia or hypoplasia of granulopoiesis. Thirteen patients were transfused therapeutically because of fever and/or severe local lesions unresponsive to antibiotic therapy. Two patients, who were transfused prophylactically after bone-marrow transplantation (BMT), were in excellent clinical condition during the entire period of granulocyte transfusions in spite of severe granulocytopenia. All patients except one showed definite clinical benefit from granulocyte transfusions. The non-responding patient had leukocyte antibodies.- Published
- 1981
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7. Cytomegalovirus (CMV) infections in patients receiving CMV-IgG-hyperimmunoglobulin prophylaxis after bone-marrow transplantation
- Author
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Schmeiser, Th., Heit, W., Arnold, R., Bunjes, D., Wiesneth, M., Hertenstein, B., Hampl, W., and Heimpel, H.
- Abstract
Conditioning therapy with aggressive chemotherapy and irradiation induces a state of transient combined immunodeficiency in bonemarrow transplant recipients. This promotes the occurrence of severe cytomegalovirus (CMV) infections, the most frequent lethal complication after bone-marrow transplantation (BMT) at present.
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- 1987
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8. Long-term remission of immune neutropenia after treatment with high-dose intravenous 7S-immunoglobulin
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Mann, K., Fateh-Moghadam, A., Pfeiffer, A., Samtleben, W., Meyer, G., Bartl, R., and Wiesneth, M.
- Abstract
Summary In a 61-year-old woman recurrent bacterial infections were caused by immune neutropenia which was resistant to high-dose cortisone and plasmapheresis. However, high-dose intravenous 7S-immunoglobulin therapy (30 g for 6 days) induced a long-term remission for 35 months. This treatment modality is safe and should be used as a first-line agent.
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- 1987
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9. Autologous platelet transfusion in alloimmunized patients with acute leukemia
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Funke, I., Wiesneth, M., Koerner, K., Cardoso, M., Seifried, E., Kubanek, B., and Heimpel, H.
- Abstract
Seventy-eight transfusions of autologous platelets were given to eight alloimmunized patients receiving curative chemotherapy for acute leukemia. Platelets were collected at regeneration of hematopoiesis after a chemotherapy cycle, cryopreserved with 5% dimethylsulfoxide in liquid nitrogen, and retransfused during bone marrow aplasia following the next treatment cycle. The in vitro platelet recovery after freezing, thawing, and washing was 85 ±4%. The in vivo corrected count increment 1 h after autologous platelet transfusions was 11±5×10
9 /l. With the exception of moderate urticaria and slight nausea each after one transfusion, no immediate or chronic side effects occurred. The bleeding time was shortened and hemorrhage during bone marrow aplasia was prevented in all alloimmunized patients by autologous platelet transfusions.- Published
- 1995
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10. Chimerism analysis after allogeneic bone marrow transplantation with nonradioactive RFLP and PCR-AFLP using the same DNA
- Author
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Schreiner, T., Prochnow-Calzia, H., Maccari, B., Erne, E., Kinzler, I., Woelpl, A., and Wiesneth, M.
- Published
- 1996
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11. Abstract
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Mache, Ch., Urban, Ch., Sauer, H., Brandesky, G., Meßner, H., Grienberger, H., Becker, H., Slave, I., Hauer, Ch., Pakisch, B., Oberbauer, R., Mokry, M., Ebner, F., Kleinert, R., Schiller, D., Kasparu, H., Schneider, G., Sega, W., Lutz, D., Mader, R. M., Steger, G. G., Sieder, A. E., Ovissi, L., Roth, E., Hamilton, G., Jakesz, R., Rainer, H., Schenk, T., Kornek, G., Schulz, F., Depisch, D., Rosen, H., Sebesta, Ch., Scheithauer, W., Locker, G. J., Czernin, J., Derfler, K., Gnant, M., Schiessel, R., Petru, E., Pickel, H., Heydarfadai, M., Lahousen, M., Haas, J., Sagaster, P., Flamm, J., Umek, H., Essl, R., Teich, G., Micksche, M., Ludwig, H., Ambros, P. F., Lestou, V., Strehl, S., Mann, G., Gadner, H., Eibl, B., Greiter, E., Grünewald, K., Gastl, G., Thaler, J., Aulitzky, W., Lion, T., Henn, T., Gaiger, A., Hofmann, J., Wolf, A., Spitaler, M., Ludescher, Christof, Grunicke, H., Mitterbauer, G., Stangl, E., Geissler, K., Jäger, U., Lechner, K., Mannhalter, C., Haas, Oskar A., Tirita, Anthi, Kahls, P., Haas, O., Hinterberger, W., Linkesch, W., Pober, Michael, Fae, Ingrid, Kyrle, Alexander, Neumeister, Andrea, Panzer, Simon, Kandioler, D., End, A., Grill, R., Karlic, H., Inhauser, T., Chott, A., Pirc-Danoewinata, H., Klepetko, W., Heinz, R., Hopfinger-Limberger, G., Koller, E., Schneider, B., Pittermann, E., Lorber, C., Eichinger, S., Neumann, E., Weidinger, J., Gisslinger, H., Bedford, P., Jones, D., Cawley, J., Catovsky, D., Bevan, P., Scherrer, R., Bettelheim, P., Knöbl, P., Kyrie, P. A., Lazcika, K., Schwarzinger, I., Sillaber, C., Watzke, H., Dávid, M., Losonczy, H., Matolcsy, A., Papp, M., Prischl, F. C., Schwarzmeier, J. D., Zoubek, Andreas, Harbott, Jochen, Ritterbach, Jutta, Ritter, Jörg, Sillaber, Ch., Agis, H., Spanblöchl, E., Sperr, W. R., Valent, P., Czerwenka, K., Virgolini, I., Li, S. R., Müller, M., Wrann, M., Gaggl, S., Fasching, B., Herold, M., Geissler, D., Nachbaur, D., Huber, Ch., Schwaighofer, H., Pichl, M., Niederwieser, D., Gilly, B., Weissel, H., Lorber, Ch., Schwarzmeier, J., Gasché, C., Reinisch, W., Hilgarth, M., Keil, F., Thomssen, C., Kolb, H. J., Holler, E., Wilmanns, W., Tilg, H., Gächter, A., Panzer-Grümayer, E. R., Majdic, O., Kersey, J. H., Petzer, A. L., Bilgeri, R., Zilian, U., Geisen, F. H., Haun, M., Konwalinka, G., Fuchs, D., Zangerle, R., Artner-Dworzak, E., Weiss, G., Fritsch, P., Tilz, G. P., Dierich, M. P., Wachter, H., Schüller, J., Czejka, M. J., Jäger, W., Meyer, B., Weiss, C., Schernthaner, G., Marosi, Ch., Onderka, E., Schlögl, B., Maca, T., Hanak, R., Mannhalter, Ch., Brenner, B., Mayer, R., Langmann, A., Langmann, G., Slave, J., Poier, E., Stücklschweiger, G., Hackl, A., Fritz, A., Pabinger, I., Willfort, A., Groiss, E., Bernhart, M., Waldner, R., Krieger, O., Nowotny, H., Strobl, H., Michlmayr, G., Mistrik, M., lstvan, L., Kapiotis, S., Laczika, K., Speiser, W., Granena, A., Hermans, J., Zwaan, F., Gratwohl, A., Labar, B., Mrsić, M., Nemet, D., Bogdanić, V., Radman, I., Zupančić-Šalek, Silva, Kovačević-Metelko, Jasna, Aurer, I., Forstinger, C., Scholten, C., Kier, P., Kalhs, P., Schwinger, W., Slavc, I., Lackner, H., Nussbaumer, W., Fritsch, E., Fink, M., Zechner, O., Kührer, I., Kletter, V., Frey, S., Leitgeb, C., Fritz, E., Silly, H., Brezinschek, R., Kuss, I., Stöger, H., Schmid, M., Samonigg, H., Wilders-Truschnig, M., Schmidt, F., Bauernhofer, T., Kasparek, A. K., Ploner, F., Stoeger, H., Moser, R., Leikauf, W., Klemm, F., Pfeffel, F., Niessner, H., Poschauko, H., Pojer, E., Locker, G. J., Braun, J., Gnant, M. F. X., Michl, I., Pirker, R., Liebhard, A., Zielinski, C., Dittrich, C., Bernát, S. I., Pongrácz, E., Kastner, J., Raderer, M., Jorbenyi, Z., Yilmaz, A., Suardet, L., Lahm, H., Odartchenko, N., Varga, Gy., Sréter, L. A., Oberberg, D., Berdel, W. E., Budiman, R., Brand, C., Berkessy, S., Radványi, G., Pauker, Zs., Nagy, Zs., Karádi, Å., Serti, S., Hainz, R., Kirchweger, P., Prager, C., Prada, J., Neifer, S., Bienzle, U., Kremsner, P., Kämmerer, B., Vetterlein, M., Pohl, W., Letnansky, K., Imre, S. G., Parkas, T., Lakos, Zs., Kiss, A., Telek, B., Felszeghy, E., Kelemen, E., Rak, K., Pfeilstöcker, M., Reisner, R., Salamon, J., Georgopoulos, A., Feistauer, S., Georgopoulos, M., Graninger, W., Klinda, F., Hrubisko, M., Sakalova, A., Weißmann, A., Röhle, R., Fortelny, R., Gutierrez, F., Fritsch, G., Printz, D., Buchinger, P., Buchinger, P., Hoecker, P., Peters, C., Gebauer, E., Katanić, D., Nagy, Á., Szomor, Á., Med., J., Batinić, D., Užaervić, B., Marušić, M., Kovačoević-Metelko, Jasminka, Jakić-Razumović, Jasminka, Kovačević-Metelko, Jasminka, Zuoancić-Šalek, Silva, Ihra, G. C., Reinisch, W. W., Hilgarth, M. F., Schwarzmeier, I. D., Várady, E., Molnár, Z. S., Fleischmann, T., Borbényi, Z., Bérczi, M., István, L., Szerafin, L., Jakó, J., Bányai, A., Dankó, K., Szegedi, Gy., Neubauer, M., Frudinger, A., Scholten, Ch., Forstinger, Ch., Dobrić, I., Willheim, M., Szépfalusi, Z., Mader, R., Boltz, G., Schwarzmeier, J. D., Nahajevszky, S., Téri, N., Póth, I., Nagy, P., Smanykó, D., Babicz, T., Ujj, Gy., Iványi, J. L., Tóth, F. D., Kiss, J., Konja, J., Petković, I., Kardum, I., Kaštelan, M., Kelečić, J., Feminić, R., Djermanović, M., Bilić, E., Jakovljević, G., Peter, B., Gredelj, G., Senji, P., Thalhammer, F., Floth, A., Etele-Hainz, A., Kainberger, F., Radaszkiewicz, T., Kierner, H., Mód, Anna, Pitlik, E., Gottesman, M., Magócsi, Mária, Sarkadi, B., Knapp, S., Purtscher, B., DelleKarth, G., Jaeger, U., Krieger, O., Berger, W., Elbling, L., Ludescher, C., Hilbe, W., Eisterer, W., Preuß, E., Izraeli, S., Janssen, J. W. G., Walther, J. U., Kovar, H., Ludwig, W. D., Rechavi, G., Bartram, C. R., Rehberger, A., Mittermayer, F., Schauer, E., Kokoschka, E. M., Kammerer, B., Kokron, E., Desser, L., Abdul-Hamid, G., Kroschinksky, F., Luther, Th., Fischer, H., Nowak, R., Wolf, H., Fleischer, J., Wichmann, G., Albercht, S., Adorf, D., Kaboth, W., Nerl, C., Aman, J., Rudolf, G., Peschel, C., Anders, O., Burstein, Ch., Ernst, B., Steiner, H., Konrad, H., Annaloro, U. P., Mozzana, C., Butti, R., Della, C., Volpe, A., Soligo, D., Uderzo, M., Lambertenghi-Deliliers, G., Ansari, H., Dickson, D., Hasford, J., Hehlmann, R., Anyanwu, E., Krysa, S., Bülzebrück, H., Vogt-Moykopf, I., Arning, M., Südhoff, Th., Kliche, K. O., Wehmeier, A., Schneider, W., Arnold, R., Bunjes, D., Hertenstein, B., Hueske, D., Stefanic, M., Theobald, M., Wiesneth, M., Heimpel, H., Waldmann, H., Arseniev, L., Bokemeyer, C., Andres, J., Könneke, A., Papageorgiou, E., Kleine, H. -D., Battmer, K., Südmeyer, I., Zaki, M., Schmoll, H. -J., Stangel, W., Poliwoda, H., Link, H., Aul, C., Runde, V., Heyll, A., Germing, U., Gattermann, N., Ebert, A., Feinendegen, L. E., Huhn, D., Bergmann, L., Dönner, H., Hartlapp, J. H., Kreiter, H., Schuhmacher, K., Schalk, T., Sparwasser, C., Peschel, U., Fraaß, C. Huber, HIadik, F., Kolbe, K., Irschick, E., Bajko, G., Wozny, T., Hansz, J., Bares, R., Buell, U., Baumann, I., Harms, H., Kuse, R., Wilms, K., Müller-Hermelink, H. K., Baurmann, H., Cherif, D., Berger, R., Becker, K., Zeller, W., Helmchen, U., Hossfeld, D. K., Bentrup, I., Plusczyk, T., Kemkes-Matthes, B., Matthes, K., Bentz, M., Speicher, M., Schröder, M., Moos, M., Döhner, H., Lichter, P., Stilgenbauer, S., Korfel, A., Harnoss, B. -M., Boese-Landgraf, J., May, E., Kreuser, E. -D., Thiel, E., Karacas, T., Jahn, B., Lautenschläger, G., Szepes, S., Fenchel, K., Mitrou, P. S., Hoelzer, D., Heil, G., Lengfelder, E., Puzicha, E., Martin, H., Beyer, J., Kleiner, S., Strohscheer, I., Schwerdtfeger, R., Schwella, N., Schmidt-Wolf, I., Siegert, W., Weyer, C., arzen, G., Risse, G., Miksits, K., Farshidfar, G., Birken, R., Schilling, C. v., Brugger, W., Holldack, J., Mertelsmann, R., Kanz, L., Blanz, J., Mewes, K., Ehninger, G., Zeller, K. -P., Böhme., A., Just, G., Bergmann., L., Shah, P., Hoelzer, D., Stille, W., Bohlen, H., Hopff, T., Kapp, U., Wolf, J., Engert, A., Diehl, V., Tesch, H., Schrader, A., van Rhee, J., Köhne-Wömpner, H., Bokemeyer', C., Gonnermann, D., Harstrick, A., Schöffski, P., van Rhee, J., Schuppert, F., Freund, M., Boos, J., Göring, M., Blaschke, G., Borstel, A., Franke, A., Hüller, G., Uhle, R., Weise, W., Brach, Marion A., Gruss, Hans-Jürgen, Herrmann, Friedhelm, deVos, Sven, Brennscheidt, Ulrich, Riedel, Detlev, Klch, Walter, Bonlfer, Renate, Mertelsmann, Roland, Brieaer, J., Appelhans, H., Brückner, S., Siemens, HJ., Wagner, T., Moecklin, W., Mertelsmann, R., Bertz, H., Hecht, T., Mertelsmann, R., Bühl, K., Eichelbaum, M. G., Ladda, E., Schumacher, K., Weimer, A., Bühling, F., Kunz, D., Lendeckel, U., Reinhold, D., Ulmer, A. J., Flad, H. -D., Ansorge, S., Bühring, Hans-Jörg, Broudy¶, Virginia C., Ashman§, Leonie K., Burk, M., Kunecke, H., Dumont, C., Meckenstock, G., Volmer, M., Bucher, M., Manegold, C., Krenpien, B., Fischer, J. R., Drings, P., Bückner, U., Donhuijsen-Ant, R., Eberhardt, B., Westerhausen, M., Busch, F. W., Jaschonek, K., Steinke, B., Calavrezos, A., Hausmann, K., Solbach, M., Woitowitz, H. -P., Hilierdal, G., Heilmann, H. -P., Chen, Z. J., Frickhofen, N., Ellbrück, D., Schwarz, T. F., Körner, K., Wiest, C., Kubanek, B., Seifried, E., Claudé, R., Brücher, J., Clemens, M. R., Bublitz, K., Bieger, O., Schmid, B., Clemetson, K. J., Clemm, Ch., Bamberg, M., Gerl, A., Weißbach, L., Danhauser-Riedl, S., Schick, H. D., Bender, R., Reuter, M., Dietzfelbinger, H., Rastetter, J., Hanauske, A. -R., Decker, Hans-Jochen, Klauck, Sabine, Seizinger, Bernd, Denfeld, Ralf, Pohl, Christoph, Renner, Christoph, Hombach, Andreas, Jung, Wolfram, Schwonzen, Martin, Pfreundschuh, Michael, Derigs, H. Günter, Boswell, H. Scott, Kühn, D., Zafferani, M., Ehrhardt, R., Fischer, K., Schmitt, M., Witt, B., Ho, A. D., Haas, R., Hunstein, W., Dölken, G., Finke, J., Lange, W., Held, M., Schalipp, E., Fauser, A. A., Mertelsmann, R., Donhuijsen, K., Nabavi, D., Leder, L. D., Haedicke, Ch., Freund, H., Hattenberger, S., Dreger, Peter, Grelle, Karen, Schmitz, Norbert, Suttorp, Meinolf, Müller-Ruchholtz, Wolfgang, Löffler, Helmut, Dumoulin, F. L., Jakschies, D., Walther, M., Hunger, P., Deicher, H., von Wussow, P., Dutcher, J. P., Ebell, W., Bender-Götze, C., Bettoni, C., Niethammer, D., Reiter, A., Sauter, S., Schrappe, M., Riehm, H., Niederle, N., Heidersdorf, H., Müller, M. R., Mengelkoch, B., Vanhoefer, U., Stahl, M., Budach, V., loehren, B., Alberti, W., Nowrousian, M. R., Seeber, S., Wilke, H., Stamatis, G., Greschuchna, D., Sack, H., Konietzko, N., Krause, B., Dopfer, R., Schmidt, H., Einsele, H., Müller, C. A., Goldmann, S. F., Grosse-Wilde, H., Waller, H. D., Libal, B., Hohaus, S., Gericke, G., von Eiff, M., Oehme, A., Roth, B., van de Loo, J., von Eiff, K., Pötter, R., Weiß, H., Suhr, B., Koch, P., Roos, H., van de Loo, J., Meuter, V., Heissig, B., Schick, F., Duda, S., Saal, J. G., Klein, R., Steidle, M., Eisner, S., Ganser, A., Seipelt, G., Leonhardt, M., Engelhard, M., Brittinger, G., Gerhartz, H., Meusers, P., Aydemir, Ü., Tintrup, W., Tiemann, H., Lennert, K., Esser, B., Hirsch, F. W., Evers, C., Riess, H., Lübbe, A., Greil, R., Köchling, A., Digel, D., Bross, K. J., Dölken, G., Mertelsmann, R., Gencic, S., Ostermann, M., Baum, R. P., Fiebig, H. H., Berger, D. P., Dengler, W. A., Winterhalter, B. R., Hendriks, H., Schwartsmann, G., Pinedo, H. M., Ternes, P., Mertelsmann, R., Dölken, G., Fischbach, W., Zidianakis, Z., Lüke, G., Kirchner, Th., Mössner, J., Fischer, Thomas, Haque, Saikh J., Kumar, Aseem, Rutherford, Michael N., Williams, Bryan R. G., Flohr, T., Decker, T., Thews, A., Hild, F., Dohmen, M., von Wussow, P., Grote-Metke, A., Otremba, B., Fonatsch, C., Binder, T., Imhof, C., Feller, A. C., Fruehauf, S., Moehle, R., Hiddemann, Th., Büchner, M. Unterhalt, Wörmann, B., Ottmann, O. G., Verbeek, G. W., Seipelt, A. Maurer, Geissler, G., Schardt, C., Reutzel, R., Hiddemann, W., Maurer, A., Hess, U., Lindemann, A., Frisch, J., Schulz, G., Mertelsmann, R., Hoelzer, P., Gassmann, W., Sperling, C., Uharek, L., Becher, R., Weh, H. J., Tirier, C., Hagemann, F. G., Fuhr, H. G., Wandt, H., Sauerland, M. C., Gause, A., Spickermann, D., Klein, S., Pfreund-schuh, M., Gebauer, W., Fallgren-Gebauer, E., Geissler, R. 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H., Dörken, B., Maschek, Hansjörg, Kaloutsi, Vassiliki, Maschek, Hansjörg, Gormitz, Ralf, Meyer, P., Kuntz, B. M. E., Mehl, B., Günther, I., Bülzebruck, H., Menssen, H. D., Mergenthaler, H. -G., Dörmer, P., Heusers, P., Zeller, K. -P., Enzinger, H. M., Neugebauer, T., Klippstein, T., Burkhardt, K. L., Putzicha, E., Möller, Peter, Henne, Christof, Eichelmann, Anette, Brüderlein, Silke, Dhein, Jens, Möstl, M., Krieger, O., Mucke, H., Schinkinger, M., Moiling, J., Daoud, A., Willgeroth, Ch., Mross, K., Bewermeier, P., Krüger, W., Peters, S., Berger, C., Bohn, C., Edler, L., Jonat, W., Queisser, W., Heidemann, E., Goebel, M., Hamm, K., Markovic-Lipkovski, J., Bitzer, G., Müller, H., Oethinger, M., Grießhammer, M., Tuner, I., Musch, E., Malek, M., Peter-Katalinic, J., Hügl, E., Helli, A., Slanicka, M., Filipowicz, A., Nissen, C., Speck, B., Nehls, M. C., Grass, H. -J., Dierbach, H., Mertelsmann, R., Thaller, J., Fiebeler, A., Schmidt, C. A., O'Bryan, J. P., Liu, E., Ritter, M., de Kant, E., Brendel, C., He, M., Dodge, R., George, S., Davey, F., Silver, R., Schiffer, C., Mayer, R., Ball, E., Bloomfield, C., Ramschak, H., Tiran, A., Truschnig-Wilders, M., Nizze, H., Bühring, U., Oelschlägel, U., Jermolow, M., Oertel, J., Weisbach, V., Zingsem, J., Wiens, M., Jessen, J., Osthoff, K., Timm, H., Wilborn, F., Bodak, K., Langmach, K., Bechstein, W., Blumhardt, G., Neuhaus, P., Olek, K., Ottinger, H., Kozole, G., Belka, C., Meusers, P., Hense, J., Papadileris, Stefan, Pasternak, G., Pasternak, L., Karsten, U., Pecherstorfer, M., Zimmer-Roth, I., Poloskey, A., Petrasch, S., Kühnemund, O., Uppenkamp, M., Lütticken, R., Kosco, M., Schmitz, J., Petrides, Petro E., Dittmann, Klaus H., Krieger, O., Pflueger, K. -H., Grueber, A., Schoeneberger, J., Wenzel, E., Havemann, K., Pies, A., Kneba, M., Edel, G., Pohl, S., Bulgay-Mörschel, M., Polzin, R., Issing, W., Clemm, Ch., Schorn, K., Ponta, H., Zöller, M., Hofmann, M., Arch, R., Heider, K. -H., Rudy, W., Tölg, C., Herrlich, P., Prümmer, O., Scherbaum, W. A., Porzsolt, F., Prümmer, O., Krüger, A., Schrezenmeier, H., Schlander, H., Pineo, G., Marin, P., Gluckman, E., Shahidi, N. T., Bacigalupo, A., Ratajczak, M. Z., Gewirtz, A. M., Ratei, R., Borner, K., Bank, U., Bühling, F., Reisbach, G., Bartke, L., Kempkes, B., Kostka, G., Ellwart, X., Birner, A., Bornkamm, G. W., Ullrich, A., Dörmer, P., Henze, G., Parwaresch, R., Müller-Weihrich, S. T., Klingebiel, Th., Odenwald, E., Brandhorst, D., Tsuruo, T., Wetter, O., Renner, C., Pohl, C., Sahin, U., Renner, U., Zeller, K. -P., Repp, R., Valerius, Th., Sendler, A., Kalden, J. R., PIatzer, E., Reuss-Borst, M. A., Bühring, H. J., Reuter, C., der Landwehr, U. Auf, der Landwehr, U. Auf, Schleyer, E., Rolf, C., Ridwelski, K., Matthias, M., Preiss, R., Riewald, M., Puzo, A., Serke, S., Rohrer, B., Pfeiffer, D., Hepp, H., Romanowski, R., Schött, C., Rüther, U., Rothe, B., Pöllmann, H., Nunnensiek, C., Schöllhammer, T., Ulshöfer, Th., Bader, H., Jipp, P., Müller, H. A. G., Rupp, W., Lüthgens, M., Eisenberger, F., Afflerbach, C., Höller, A., Schwamborn, J. S., Daus, H., Krämer, K., Pees, H., Salat, C., Reinhardt, B., Düll, T., Knabe, H., Hiller, E., Sawinski, K., Schalhorn, A., Kühl, M., Heil, K., Schardt, Ch., Drexler, H. G., Scharf, R. E., Suhijar, D., del Zoppo, G. J., Ruggeri, Z. M., Roll, T., Möhler, T., Giselinger, H., Knäbl, P., Kyrie, P. A., Lazcíka, K., Lechner, X., Scheulen, M. E., Beelen, D. W., Reithmayer, H., Daniels, R., Weiherich, A., Quabeck, K., Schaefer, U. W., Reinhardt, J., Grimm, M., Unterhalt, M., Schliesser, G., Lohmeyer, J., Schlingheider, O., von Eiff, M., Schulze, F., Oehme, C., van de Loo, J., Schlögl, E., Bemhart, M., Schmeiser, Th., Rozdzinski, E., Kern, W., Reichle, A., Moritz, T., Merk, Bruno, Schmid, R. M., Perkins, N. D., Duckett, C. S., Leung, K., Nabel, G. J., Pawlaczyk-Peter, B., Kellermann-Kegreiß, Schmidt E., Steiert, I., Schmidt-Wolf, G., Schmidt-Wolf, I. G. H., Schlegel, P., Blume, K. G., Chao, N. J., Lefterova, P., Laser, J., Schmitz, G., Rothe, G., Schönfeld, S., Schulz, S., Nyce, J. W., Graf, N., Ludwig, R., Steinhauser, I., Brommer, A. E., Qui, H., Schroeder, M., Grote-Kiehn, J., Bückner, U., Rüger, I., Schröder, J., Meusers, P., Weimar, Ch., Schoch, C., Schröter, G., Stern, H., Buchwald, B., Schick, K., Avril, N., Flierdt, E. v. d., Langhammer, H. R., Pabst, H. W., Alvarado, M., Witte, T., Vogt, H., Schuler, U., Brammer, K., Klann, R. C., Schumm, M., Hahn, J., Günther, W., Wullich, B., Moringlane, J. R., Schöndorf, S., Schwartz, S., Bühring, H. -J., Notter, M., Böttcher, S., Martin, M., Schmid, H., Lübbe, A. S., Leib-Mösch, C., Wankmüller, H., Eilbrück, D., Funke, I., Cardoso, M., Duranceyk, H., Seitz, R., Rappe, N., Kraus, H., Egbring, R., Haasberg, M., Havemann, K., Seibach, J., Wollscheid, Ursula, Serke, St., Zimmermann, R., Shirai, T., Umeda, M., Anno, S., Kosuge, T., Katoh, M., Moro, S., Su, C. -Y., Shikoshi, K., Arai, N., Schwieder, G., Silling-Engelhardt, G., Zühlsdorf, M., Aguion-Freire-Innig, E., van de Loo, J., Stockdreher, K., Gatsch, L., Tischler, H. -J., Ringe, B., Diedrich, H., Franzi, A., Kruse, E., Lück, R., Trenn, G., Sykora, J., Wen, T., Fung-Leung, W. P., Mak, T. W., Brady, G., Loke, S., Cossman, J., Gascoyne, R., Mak, T., Urasinski, I., Zdziarska, B., Usnarska-Zubkiewicz, L., Kotlarek-Haus, S., Sciborskl, R., Nowosad, H., Kummer, G., Schleucher, N., Preusser, P., Niebel, W., Achterrath, W., Pott, D., Eigler, F. -W., Venook, A., Stagg, R., Frye, J., Gordon, R., Ring, E., Verschuer, U. v., Baur, F., Heit, W., Corrons, J. L. L. Vives, Vogel, M., Nekarda, H., Remy, W., Bissery, M. C., Aapro, M., Buchwald-Pospiech, A., Kaltwasser, J. P., Jacobi, V., de Vos, Sven, Asano, Yoshinobu, Voss, Harald, Knuth, Alexander, Wiedemann, G., Komischke, B., Horisberger, R., Wussow, P. v., Wanders, L., Senekowitsch, R., Strohmeyer, S., Emmerich, B., Selbach, J., Gutensohn, K., Wacker-Backhaus, G., Winkeimann, M., Send, W., Rösche, J., Weide, R., Parviz, B., Havemann, K., Weidmann, B., Henss, H., Engelhardt, R., Bernards, P., Zeidler, D., Jägerbauer, E., Colajori, E., Kerpel-Fronius, S., Weiss, A., Buchheidt, D., Döring, A., D.Saeger, H., Weissbach, L., Emmler, J., Wermes, R., Meusers, P., Flasshove, M., Skorzec, M., Käding, J., Platow, S., Winkler, Ute, Thorpe, Philip, Winter, S. F., Minna, J. D., Nestor, P. J., Johnson, B. E., Gazdar, A. F., Havemann, K., Carbone, D. P., Wit, M. de, Bittner, S., Hossfeld, D., Wittmann, G., Borchelt, M., Steinhagen-Thiessen, E., Koch, K., Brosch, T., Haas, N., Wölfel, C., Knuth, A., Wölfel, T., Safford, M., Könemann, S., Zurlutter, K., Schreiber, K., Piechotka, K., Drescher, M., Toepker, S., Terstappen, L. W. M. M., Bullerdiek, J., Jox, A., zur Hausen, H., Wolters, B., Stenzinger, W., Woźny, T., Sawiński, K., Kozłowska-Skrzypczak, M., Wussow, P. v., Hochhaus, T., Ansarl, H., Prümmer, O., Zapf, H., Thorban, S., Präuer, H., Zeller, W., Stieglitz, J. v., Dürken, M., Greenshaw, C., Kabisch, H., Reuther, C., Knabbe, C., Lippman, M., Havemann, K., Wellstein, A., Degos, L., Castaigne, S., Fenaux, P., Chomienne, C., Raza, A., and Preisler, H. D.
- Published
- 1992
- Full Text
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12. Parvovirus B19 infection and bone marrow transplantation
- Author
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Frickhofen, N., Arnold, R., Hertenstein, B., Wiesneth, M., and Young, N. S.
- Abstract
Parvovirus B19 lytically infects erythroid progenitor cells and thereby causes cessation of erythropoiesis in infected individuals. Anemia develops only if red cell turnover is increased, as in patients with chronic hemolysis (transient aplastic crisis). In addition to transient marrow failure, B19 can cause chronic anemia and, rarely, pancytopenia in immunodeficient patients who are not able to mount an adequate immune response to clear the virus. Bone marrow transplantation, although causing significant immunosuppression, is rarely complicated by symptomatic B19 infection. This is probably due to effective passive immunotherapy by immunoglobulin infusions immediately after transplantation and early reconstitution of antibody responses after uncomplicated transplantation.
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- 1992
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13. Low incidence of invasive fungal infections after bone marrow transplantation in patients receiving amphotericin B inhalations during neutropenia
- Author
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Hertenstein, B., Kern, W. V., Schmeiser, T., Stefanic, M., Bunjes, D., Wiesneth, M., Novotny, J., Heimpel, H., and Arnold, R.
- Abstract
Summary The incidence of invasive fungal infections after bone marrow transplantation (BMT) was analyzed in 303 consecutive marrow graft recipients (allogeneicn=271, autologousn=27, syngeneicn=5). All patients received inhalations with amphotericin B (10 mg twice daily) during neutropenia. The overall incidence of invasive fungal infections within the first 120 days after transplant was 3.6% (11/303; aspergillosis: 6; yeast infection: 5). Four of the 11 cases occurred early, and seven cases were observed after neutrophil recovery and discontinuation of amphotericin B inhalation treatment. Late infection was significantly associated with the development of acute graft-versus-host disease. Four of the 11 infections (early 2/4; late: 2/7) were observed in patients with a history of previous fungal infection. Other patient and treatment characteristics were not helpful in defining potential risk factors. In particular, the incidence of invasive fungal infections did not differ between patients with more or less strict reverse isolation measures. Occasional side effects such as initial mild cough and bad taste were rare, usually disappeared during continued administration, and were in no case the reason for discontinuation of treatment. These data suggest that aerosolized amphotericin B may be a useful, convenient, and efficient prophylactic antifungal regimen in BMT.
- Published
- 1994
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14. Treatment of refractory chronic idiopathic thrombocytopenic purpura with high dose intravenous immunoglobulin
- Author
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Seifried, E., Pindur, G., Stötter, H., Wiesneth, M., Rasche, H., and Heimpel, H.
- Abstract
Three patients with a history of chronic idiopathic thrombocytopenic purpura stretching back over 20 years are reported. Despite splenectomy and immunosuppressive therapy satisfactory control of their disease has not been achieved. They had remained refractory to all therapeutic manoeuvres with corticosteroids and immunosuppressives for years with thrombocyte counts between 5,000 and 25,000/µl and the concommitant risk of bleeding.
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- 1984
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15. A simple technique for processing of bone marrow for human marrow transplantation
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Pflieger, H., Wiesneth, M., Schmeiser, T., and Kubanek, B.
- Abstract
A simple stainless steel device is described for the processing of marrow for intravenous infusion to marrow graft recipients. The device can be cleaned easily and sterilized for re-use.
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- 1982
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16. EVIDENCE FOR THE INVOLVEMENT OF HOSTDERIVED OKT8POSITIVE T CELLS IN THE REJECTION OF TDEPLETED HLAIDENTICAL BONE MARROW GRAFTS
- Author
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BUNJES, D., HEIT, W., ARNOLD, R., SCHMEISER, T., WIESNETH, M., CARBONELL, F., PORZSOLT, F., RAGHAVACHAR, A., and HEIMPEL, H.
- Abstract
The recent introduction of a variety of techniques for removing T cells from bone marrow grafts has reduced the incidence of graft-versus-host disease (GVHD)-associated morbidity and mortality (1–4). Whether this advance will be translated into improved patient survival is unclear at present, mainly because these procedures increase the risk of graft failure (2, 5,6).
- Published
- 1987
17. Gallstone Prevalence in Relation to Smoking, Alcohol, Coffee Consumption, and Nutrition: The Ulm Gallstone Study
- Author
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Kratzer, W., Kächele, V., Mason, R. A., Muche, R., Hay, B., Wiesneth, M., Hill, V., Beckh, K., and Adler, G.
- Abstract
Background: Besides considering well-known risk factors for the development of gallbladder stones, such as age, sex, fecundity, and hereditary predisposition, efforts at prevention have focused increasingly on other factors, such as nicotine, alcohol, and caffeine consumption, as well as general nutrition, which may be modified. Methods: A total of 1116 blood donors were examined between April 1994 and February 1995 in the central blood bank of the German Red Cross in Ulm, Germany. Each subject received a questionnaire and underwent to an upper abdominal ultrasound examination. Results: Gallbladder stone disease (current cholecystolithiasis and history of cholecystectomy) was detected in 5.8% of the men and 6.3% of the women. Neither regularity nor number of daily meals correlated with the frequency of gallstone disease. Vegetarians (n = 48), as a group, were not found to have gallstones. In relation to the consumption of alcohol, tobacco, or caffeine, a slightly higher prevalence of cholecystolithiasis was found only in heavy drinkers of coffee (P= 0.051; odds ratio (OR), 1.083; 95% confidence interval (CI), 0.999, 1.174). Conclusion: Results of the present study do not show a definite relationship between nutritional factors and the consumption of alcohol, tobacco, or caffeine and an increased prevalence of gallbladder stone disease.
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- 1997
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18. Hepatitis C virus infection is a risk factor for liver failure from veno-occlusive disease after bone marrow transplantation [see comments]
- Author
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Frickhofen, N, Wiesneth, M, Jainta, C, Hertenstein, B, Heymer, B, Bianchi, L, Dienes, HP, Koerner, K, Bunjes, D, and Arnold, R
- Abstract
The contribution of hepatitis C virus (HCV) infection to liver disease after bone marrow transplantation (BMT) was retrospectively evaluated in 61 patients treated with BMT. HCV genome, as well as antibodies to HCV, was analyzed in sera collected before and serially after BMT. Six patients had been infected with HCV before BMT and three patients acquired the infection during or shortly after BMT. All patients infected before BMT died within 10 weeks after transplantation. Five of these six patients (83%) died of veno-occlusive disease (VOD), compared with nine of 52 patients (17%) not infected with HCV (P < .005). Risk factors for VOD other than HCV were not more prevalent in these patients compared with uninfected patients. Parallel to the development of VOD, replication of HCV increased, as demonstrated by rising concentrations of viral RNA in serum. HCV infection acquired during or after BMT caused only mild acute hepatitis C, which progressed to chronic hepatitis C in one patient surviving 10 years after BMT. These data suggest that patients with liver disease caused by HCV infection are at high risk of developing lethal VOD after BMT.
- Published
- 1994
- Full Text
- View/download PDF
19. Infectious complications after allogeneic bone marrow transplantation with and without T-cell depletion of donor marrow
- Author
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Schmeiser, T., Wiesneth, M., Bunjes, D., Arnold, Renate, Hertenstein, B., Heit, W., and Kurrte, E.
- Abstract
Summary The infectious complications during different time intervals after allogeneic bone marrow transplantation (BMT) (day 0 to day 30, 31 to 100, 101 to 365, 366 to 730) were reviewed in 67 adult patients, 27 of whom received transplants without T-cell depletion (TCD) using methotrexate or cyclosporin A for prophylaxis of graft-versus-host disease (GvHD) and 40 of whom received donor marrow with TCD using the monoclonal anti-lymphocyte antibody campath-1 and human complement. The use of TCD reduced the incidence and severity of GvHD significantly (p<0.01), but was associated with an increased rate of graft rejections. During all time intervals patients with TCD had a similar, lower or statistically significantly lower number of bacterial, fungal or viral infections and a statistically significantly lower number of lethal infections (p=0.05) as compared with patients without TCD. This finding might be explained by the fact that with TCD immunological reconstitution can take place unimpaired by GvHD or its prophylaxis or treatment, resulting in a decreased incidence of infections.
- Published
- 1989
- Full Text
- View/download PDF
20. Mobilization and Collection of Allogeneic and Autologous Peripheral Blood Progenitor Cells for Transplantation
- Author
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Wiesneth, M., Schreiner, T., Frickhofen, N., Friedrich, W., Müller, S., and Kubanek, B.
- Abstract
Bei 10 Patienten mit Non-Hodgkin-Lymphomen wurden nach Chemotherapie und 5µg G-CSF/kg Körpergewicht und Tag insgesamt 28 Blutstammzellapheresen durchgeführt. Im Vergleich hierzu erfolgten bei 8 Normalpersonen mit täglich 5 bzw. 10 µg G-CSF/kg am Tag 5 und 6 insgesamt 16 Apheresen. Bei den Patienten und den Spendern mit 10 µg G-CSF kam es zu einer adäquaten Mobilisation von CD34
+ Zellen im peripheren Blut (xxx > 200/µl). Die Apheresedauer betrug im Mittel 175 (143-201) min mit einem Separations-volumen von 8,9 (6,9-11,1) Liter Blut. Die Transplantat-Sollzahlen von 5 × 108 mononukleären Zellen (MNC), 5 × 106 CD34+ Zellen und 1 × 105 CFU-GM/kg konnten bei Patienten nach Chemotherapie und 5 µg G-CSF wie auch bei Spendern mit der höheren Dosis von 10 µg G-CSF durch < 2 Standardapheresen erreicht wer-den. Die Konditionierung mit 10 µg G-CSF/kg führte jedoch zu Nebenwirkungen, so daβ Alternativen wie die In-vitro Expansion zur Präparation von allogenen Blutstammzellen zur Transplantation angestrebt werden sollten.- Published
- 2010
- Full Text
- View/download PDF
21. T-Cell Depletion of Allogeneic Peripheral Blood Progenitor Cells for HLA-Nonidentical Transplantation in Children
- Author
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Schreiner, T., Wiesneth, M., Maccari, B., Erne, E., Bischof, C., Friedrich, W., Müller, S., and Kubanek, B.
- Abstract
Bei Patienten mit malignen Systemerkrankungen ohne HLA-kompatiblen Kno-chenmarkspender wurde neben Knochenmark die zusätzliche Transplantation T-Zell-depletierter peripherer Blutstammzellen von nichtidenten Familienspendern durchgeführt. Die primär verwendete Lektin/Rosetten-Methode führte nicht immer zu einer ausreichenden Depletion von T-Lymphozyten der allogenen Blutstamm-zellpräparate. Deshalb wurde eine Kombination aus primärer Selektion von CD34+-Zellen und anschlieβender immunmagnetischer T-Zell-Depletion entwickelt. Dieses neue Verfahren reduzierte bei einem guten Ertrag von Stammzellen die T-Lymphozyten unter 1 × 105 pro kg Körpergewicht. Vier Kinder wurden mit einer Kombination aus Knochenmark und peripheren Stammzellen bei 3 bis 4HLA-Dif-ferenzen transplantiert und zeigten eine rasche hämatopoetische Regeneration ohne schwere Komplikationen einer Spender-gegen-Wirt-Reaktion.
- Published
- 2010
- Full Text
- View/download PDF
22. HEMISPHERIC STROKE DOES NOT MOBILIZE CD34HEMATOPOIETIC STEM CELLS INTO THE PERIPHERAL BLOOD
- Author
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Höchsmann, B, Huber, R, Schauwecker, P, Schrezenmeier, H, Wiesneth, M, and Storch, A
- Published
- 2009
- Full Text
- View/download PDF
23. Highly effective CD34+ selection of granulocyte colony-stimulating factor-mobilized allogeneic peripheral blood progenitor cells [letter]
- Author
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Schreiner, T, Maccari, B, Erne, E, Bischof, C, and Wiesneth, M
- Published
- 1996
- Full Text
- View/download PDF
24. Treatment of chronic granulocytic leukaemia (CGL) with allogeneic bone-marrow transplantation (BMT)
- Author
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Schmeiser, Th., Wiesneth, M., Arnoid, R., Bunjes, D., Hertenstein, B., Heimpel, H., and Heit, W.
- Published
- 1986
- Full Text
- View/download PDF
25. A simplified rapid method for restriction fragment length polymorphism analysis after bone marrow transplantation
- Author
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Schreiner, T., Wiesneth, M., Maccari, B., and Sawodny, B.
- Published
- 1994
- Full Text
- View/download PDF
26. Clinical relevance of endocrine and reproductive dysfunctions in patients after bone marrow transplantation
- Author
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HETZEL, W.D., KREUSER, E.D., SCHMEISER, TH., BRÄNDLE, E., WIESNETH, M., HERTENSTEIN, B., ARNOLD, R., HEIT, W., and HEIMPEL, H.
- Published
- 1988
- Full Text
- View/download PDF
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