Liu, Zhaochao, Zhou, Lizhou, Gan, Chenchen, Hu, Lijuan, Pang, Biao, Zuo, Dan, Wang, Guangyi, Wang, Hongcheng, and Liu, Yingliang
Soil and water are increasingly at risk of contamination from the toxic heavy metals lead (Pb) and cadmium (Cd). Arabis paniculata (Brassicaceae) is a hyperaccumulator of heavy metals (HMs) found widely distributed in areas impacts by mining activities. However, the mechanism by which A. paniculata tolerates HMs is still uncharacterized. For this experiment, we employed RNA sequencing (RNA-seq) in order to find Cd (0.25 mM)- and Pb (2.50 mM)-coresponsive genes A. paniculata. In total, 4490 and 1804 differentially expressed genes (DEGs) were identified in root tissue, and 955 and 2209 DEGs were identified in shoot tissue, after Cd and Pb exposure, respectively. Interestingly in root tissue, gene expression corresponded similarly to both Cd and Pd exposure, of which 27.48% were co-upregulated and 41.00% were co-downregulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses showed that the co-regulated genes were predominantly involved in transcription factors (TFs), cell wall biosynthesis, metal transport, plant hormone signal transduction, and antioxidant enzyme activity. Many critical Pb/Cd-induced DEGs involved in phytohormone biosynthesis and signal transduction, HM transport, and transcription factors were also identified. Especially the gene ABCC9 was co-downregulated in root tissues but co-upregulated in shoot tissues. The co-downregulation of ABCC9 in the roots prevented Cd and Pb from entering the vacuole rather than the cytoplasm for transporting HMs to shoots. While in shoots, the ABCC9 co-upregulated results in vacuolar Cd and Pb accumulation, which may explain why A. paniculata is a hyperaccumulator. These results will help to reveal the molecular and physiological processes underlying tolerance to HM exposure in the hyperaccumulator A. paniculata , and aid in future efforts to utilize this plant in phytoremediation. [Display omitted] • RNA sequencing was used for the first time to find the Cd- and Pb- coresponsive genes in Arabis paniculata. • Arabis paniculata is a hyperaccumulator of heavy metals found widely distributed in areas impacts by mining activities. • The co-regulated genes were predominantly involved in TFs, cell wall biosynthesis, metal transport, plant hormone signal transduction, and antioxidant enzyme activity. • Many critical Cd/Pb-induced DEGs, such as HAI2 , ABF3 , YSL3 , ZIP11 , ABCC9 , NRAMP4 , EIN3 , WRKY19 , and WRKY40 , were also identified. • The creation of a PPI network facilitated the identification of key genes, such as PI4KG4 , MPK1 and XLG2. [ABSTRACT FROM AUTHOR]