Search

Two hetero-tri-organometallic compounds with potent activity against Gram-positive bacteria including multi-resistant Staphylococcus aureus(MRSA) were identified. The compounds consist of a peptide nucleic acid backbone with an alkyne side chain, substituted with a cymantrene, a (dipicolyl)Re(CO)3moiety, and either a ferrocene (FcPNA) or a ruthenocene (RcPNA). Comparative proteomic analysis indicates the bacterial membrane as antibiotic target structure. FcPNA accumulation in the membrane was confirmed by manganese tracing with atomic absorption spectroscopy. Both organometallics disturbed several essential cellular processes taking place at the membrane such as respiration and cell wall biosynthesis, suggesting that the compounds affect membrane architecture. Correlating with enhanced antibacterial activity, oxidative stress was induced only by the ferrocene-substituted compound. The organometallics described here target the cytoplasmic membrane, a clinically proven antibacterial target structure, feature a bactericidal but non-bacteriolytic mode of action and limited cytotoxicity within the limits of solubility. Thus, FcPNA represents a promising lead structure for the development of a new synthetic class of antibiotics.

The article discusses the effectiveness of school leveling program among the students in Walla Walla (WA) School District. It notes the three level intervention approach comprise of a series of distinguished instructional plans that points to the different levels and introduces the response to intervention (RTI) model. It mentions the RTI framework which includes the different philosophy and features of each level. Statistical information about Walla Walla (WA) High School is also presented.

The article features the three-tiered intervention educational program of Walla Walla (WA) School District in Washington. It provides information on the significant distribution of the instruction program on the academic performance of the students handled by Professor Peder Fretheim. It states that the program also handles behavioral issues aside from academic.

Hemispheric asymmetries, fourth venticular size, and cerebellar morphology were examined in 15 healthy men, aged 18 to 39 years, with documented childhood diagnoses of infantile autism, and in 20 healthy age-and sex-matched controls using computerized transverse axial tomography (CT). Nine patients were of approximately average intelligence, 3 showed specific language impairments, and 3 were mentally retarded. No significant group differences were seen in the distributions of frontal or posterior asymmetries of width or petalia. No subject showed evidence of cerebellar atrophy or an enlarged fourth ventricle. These results fail to support a hypothesis of unusual hemispheric asymmetry or macroscopic abnormalities of the posterior fossa in autism.

New antibiotics are urgently needed to address the mounting resistance challenge. In early drug discovery, one of the bottlenecks is the elucidation of targets and mechanisms. To accelerate antibiotic research, we provide a proteomic approach for the rapid classification of compounds into those with precedented and unprecedented modes of action. We established a proteomic response library of Bacillus subtiliscovering 91 antibiotics and comparator compounds, and a mathematical approach was developed to aid data analysis.