1. Micellar Liquid Chromatography of Plant Extracts Components
- Author
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Stępnik, Katarzyna E., Malinowska, Irena, and Rój, Edward
- Abstract
Raw materials quality assessment is a very important factor, enabling products creation up to the international standards. In this investigation, the qualitative composition of bioactive plant extracts has been checked. The tested extracts have been obtained by the use of supercritical carbon dioxide extraction and can be utilized as raw materials in a commercial production of a wide range of many different goods. Plant extracts of raspberry (Rubus idaeus), strawberry (Fragaria ananassa), blackcurrant (Ribes nigrum), aronia (Aronia Medik.), Japanese rose (Rosa rugosaThunb.) seeds, and palmetto palm (Sabal minor) fruit have been examined. In these extracts, saturated, monounsaturated, and polyunsaturated fatty acids as well as polyphenols have been identified using thin-layer chromatography (TLC) with aqueous—organic mobile phases. Appropriate content of these components tends to be a determinant of human organism operation. In our investigation, the plant extract components, especially saturated and unsaturated fatty acids and polyphenols, have been tested using micellar chromatography. Different micellar environments, containing polioxyethylene (23) lauryl ether (Brij35), sodium dodecyl sulfate (SDS), and cetyltrimethylammonium bromide (CTAB) as a main component of micellar mobile phases, have been utilized. The analyte—micelle association constants (Kma) from Foley’s equation have been compared for each of these environments.
- Published
- 2014
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