1. The use of aged stool specimens for the detection of rotavirus.
- Author
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de Bruyn, Karin, Theron, Elizabeth M. C., Dewar, John B., and Hendrick, Richard M.
- Subjects
ENZYME-linked immunosorbent assay ,VIRUS diseases ,CHILD death ,ROTAVIRUSES ,HUMAN body ,DEFECATION - Abstract
Background: Rotavirus is considered worldwide as one of the most important viral gastrointestinal infections, resulting in potentially life-threatening diarrhoea and death in children under the age of 5 years. Rotavirus can survive and remain infectious for long periods outside of the human body and can be easily transmitted via environmental surfaces. Method: Stool specimens that had been collected and stored since 2010/2011 at 2°C - 8°C instead of -20°C or -80°C were analysed to determine the viability of rotavirus in these specimens after 6 years of improper storage. The specimens were analysed using simple enzyme immunoassay (EIA) methods from two different suppliers at different times throughout the period (2012-2017). Results: The analysis showed similar detection results for the two EIA kits. Conclusion: The rotavirus can be detected after several years of incorrect storage with EIA kits. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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