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Probiotic strains are phrased as beneficial for life because this bacterial association has substantial effects on human and animal health when administrated orally, among which bio-active metabolite short-chain fatty acids production and cholesterol assimilation are most significant. All the short-chain fatty acids, mainly acetate, propionate, and butyrate reveal valuable effects on human health. In this research, the ability of 9 the potential lactic acid bacteria food-isolated probiotics was investigated to produce bio-active metabolite short-chain fatty acids directly or indirectly, and cholesterol-lowering ability through various mechanisms. Their 16S rRNA gene sequences were analyzed. short-chain fatty acids were quantified by gas chromatography, while cholesterol assimilation was measured using the o -phthalaldehyde method with slight modifications. All isolated strains through this study have the ability to optimize cholesterol content in the culture broth, short-chain fatty acid production, and survival in extreme alkaline and acidic conditions. The highest resistance to bile salt concentrations, γ-hemolysis, and strong antibacterial activity against pathogenic indicators, especially Escherichia coliATCC25922. The highest Lipid accumulation inhibitory effect was noticed in Lentilactobacillus hilgardii LMG 07934 as a potential probiotic isolate on the basis of cholesterol assimilation capacity (i.e., 91.4%), along with the ability to produce abundant i.e., butyric acid, propionic acid, and acetic acid, followed by Lacticaseibacillus paracasei ATCC 334 which has 85.3% cholesterol assimilation ability. These findings demonstrated that these isolated strains from Chinese fermented rice can be used as a potential probiotic and alternate therapeutic supplement because of their ability to produce short-chain fatty acids and their cholesterol-lowering ability. [Display omitted] • Nine fermented rice-isolated Lactic acid bacteria were screened for their potential probiotic properties. • Short-chain fatty acid production ability i.e., acetate, propionate, and butyrate. • Cholesterol assimilation ability of all probiotics investigated. • Survival in extreme alkaline and acidic conditions. • Strong antibacterial activity against pathogenic indicators, especially Escherichia coliATCC25922. [ABSTRACT FROM AUTHOR]