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2 results on '"Shloma, Victor V."'

1. Electron Microscopy of Polytene Chromosomes.

Author

Walker, John M., Henderson, Daryl S., Semeshin, Valery F., Belyaeva, Elena S., Shloma, Victor V., and Zhimulev, Igor F.

Abstract

Most studies of the structure and function of Dipteran salivary gland poly-tene chromosomes are based on the phenomenon of the relative constancy of the banding pattern characteristic of each species. This made possible the building of cytological maps of polytene chromosomes widely used in genetic and molecular genetic research. The most detailed maps of the salivary gland polytene chromosomes of Drosophila melanogaster were those made in the 1930s to 1940s by Bridges and Bridges using the light microscope (LM) (seeref. 1). More recently, Sorsa and his colleagues (2-4) presented revised electron microscope (EM) maps covering the entire salivary gland chromosome set of D. melanogaster. To carry out this extremely laborious work, they used a squash/thin-sectioning EM technique. We consider this approach to be the best for precise mapping of politene chromosome regions. Moreover, chromosomes prepared by this method can be used for autoradiographic study, in situ hybridization and protein localization at the EM level, as described in this chapter. [ABSTRACT FROM AUTHOR]

Published
2004
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2. DNA Copy-Number Control through Inhibition of Replication Fork Progression

Author

Nordman, Jared T., Kozhevnikova, Elena N., Verrijzer, C. Peter, Pindyurin, Alexey V., Andreyeva, Evgeniya N., Shloma, Victor V., Zhimulev, Igor F., and Orr-Weaver, Terry L.

Abstract

Proper control of DNA replication is essential to ensure faithful transmission of genetic material and prevent chromosomal aberrations that can drive cancer progression and developmental disorders. DNA replication is regulated primarily at the level of initiation and is under strict cell-cycle regulation. Importantly, DNA replication is highly influenced by developmental cues. In Drosophila, specific regions of the genome are repressed for DNA replication during differentiation by the SNF2 domain-containing protein SUUR through an unknown mechanism. We demonstrate that SUUR is recruited to active replication forks and mediates the repression of DNA replication by directly inhibiting replication fork progression instead of functioning as a replication fork barrier. Mass spectrometry identification of SUUR-associated proteins identified the replicative helicase member CDC45 as a SUUR-associated protein, supporting a role for SUUR directly at replication forks. Our results reveal that control of eukaryotic DNA copy number can occur through the inhibition of replication fork progression.

Published
2014
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