1. TIF1β activates leukemic transcriptional program in HSCs and promotes BCR::ABL1-induced myeloid leukemia
- Author
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Morii, Mariko, Kubota, Sho, Iimori, Mihoko, Yokomizo-Nakano, Takako, Hamashima, Ai, Bai, Jie, Nishimura, Akiho, Tasaki, Masayoshi, Ando, Yukio, Araki, Kimi, and Sashida, Goro
- Abstract
TIF1β/KAP1/TRIM28, a chromatin modulator, both represses and activates the transcription of genes in normal and malignant cells. Analyses of datasets on leukemia patients revealed that the expression level of TIF1βwas increased in patients with chronic myeloid leukemia at the blast crisis and acute myeloid leukemia. We generated a BCR::ABL1conditional knock-in (KI) mouse model, which developed aggressive myeloid leukemia, and demonstrated that the deletion of the Tif1βgene inhibited the progression of myeloid leukemia and showed longer survival than that in BCR::ABL1KI mice, suggesting that Tif1βdrove the progression of BCR::ABL1-induced leukemia. In addition, the deletion of Tif1βsensitized BCR::ABL1KI leukemic cells to dasatinib. The deletion of Tif1βdecreased the expression levels of TIF1β-target genes and chromatin accessibility peaks enriched with the Fosl1-binding motif in BCR::ABL1KI stem cells. TIF1β directly bound to the promoters of proliferation genes, such as FOSL1, in human BCR::ABL1cells, in which TIF1β and FOSL1 bound to adjacent regions of chromatin. Since the expression of Fosl1was critical for the enhanced growth of BCR::ABL1KI cells, Tif1β and Fosl1 interacted to activate the leukemic transcriptional program in and cellular function of BCR::ABL1KI stem cells and drove the progression of myeloid leukemia.
- Published
- 2024
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