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AbstractWe developed a confocal laser scanning microscope for intra-operative imaging of brain tumors using near infrared fluorescence. The quality of near infrared images of Indocyanine Green (ICG) was compared with the surgical confocal scanning (SCS) microscope and a conventional charge-coupled device (CCD) camera; we compared images of a tube filled with ICG, which was located in the mouse brain. Compared to the CCD camera, the SCS microscope could obtain a more precise image of ICG fluorescence through the brain tissue. In addition, the SCS microscope could image ICG fluorescence clearly in a relatively light room because of elimination of stray light, while the CCD camera required high darkness to obtain ICC images. The present SCS microscope can give useful intra-operative imaging of brain tumors, particularly detection of residual tumor tissues that extend into normal brain tissues. [Neurol Res 2000; 22: 533-536]

The use of positron-emission tomography (PET) has shown a variety of age-related alterations in neuronal activity in elderly patients. Near infrared spectroscopy (NIRS) has shown a multiplicity of evoked cerebral blood oxygenation (CBO) changes during cognitive performance. To evaluate the effects of aging on the CBO changes, we compared the CBO changes induced by cognitive tasks between young adults and older adults by using NIRS. Twenty-six participants performed 6 cognitive tasks to evaluate differences among young adults (mean age+/-SD, 28.8+/-4.4 years) and older adults (50.7+/-8.0 years). We monitored CBO changes in the left prefrontal cortex. We classified several common patterns of the NIRS parameter change and compared the frequency of each pattern in both groups. In both groups, the most common NIRS parameter change was an increase in oxyhemoglobin (Oxy-Hb) and total-hemoglobin (Total-Hb), with a decrease in deoxyhemoglobin (Deoxy-Hb). The frequency of this pattern in the older group, however, was considerably lower than in the young group (P<.01). In addition, decreases of Oxy-Hb and Total-Hb during the tasks were more common in the older groups, (P<.0001), suggesting a decrease of regional cerebral blood flow during the tasks. Age effects varied for specific tasks, with results being significant for verbal fluency and reading, (P<.05). During all tasks, older subjects were less likely than younger subjects to have the typical pattern of increases in Oxy-Hb and Total-Hb. In addition, compared with younger subjects, older subjects had a greater frequency of decreases in Oxy-Hb and Total-Hb. The overall results, as well as results for specific tasks, suggest that functional reorganization or alteration of CBO responses may occur during aging.

Objective Blood oxygenation level dependent (BOLD) contrast functional MRI images show activated cortical areas by detecting a reduced concentration of deoxyhaemoglobin (deoxy-Hb) during neuronal activity; however, near infrared spectroscopy (NIRS) has shown various patterns of cerebral blood oxygenation (CBO) changes in the frontal lobe during cognitive tasks. To determine if various patterns of CBO changes occur in the frontal lobe when the brain is directly stimulated, changes in CBO in the frontal lobe induced by deep brain stimulation in patients with implanted electrodes were evaluated. Methods Six patients were studied, including five with Parkinson's disease and one with essential tremor. To reduce tremor or rigidity, the electrodes were implanted at the thalamic nucleus ventralis intermedius (VIM: three Parkinson's disease and one essential tremor) or the globus pallidus internus (GPi: two Parkinson's disease). Using NIRS, changes of deoxy-Hb, oxyhaemoglobin (oxy-Hb) and total haemoglobin (total Hb) were measured in the bilateral frontal lobes during various stimulus conditions. Results High frequency (120 Hz) GPi stimulation consistently increased oxy-Hb and total Hb with a decrease of deoxy-Hb in an intensity and time dependent manner. Oxy-Hb and total Hb increased immediately after the onset of stimulation and then gradually decreased when stimulation was continued. By contrast, high frequency (120 Hz) VIM stimulation decreased oxy-Hb, deoxy Hb and total Hb in an intensity dependent manner. In the severe tremor patient with VIM stimulation, frequency response was examined by decreasing stimulus frequencies; deoxy-Hb increased at high frequencies (70-40 Hz), and then decreased below the control level at low frequencies (30-0 Hz), whereas oxy-Hb and total Hb increased consistently at high and low frequencies. Conclusion The electrical stimulation of GPi and VIM caused various CBO changes in the frontal lobe, which were similar to those found during cognitive tasks. Such a multiplicity of CBO changes in the frontal lobe may be caused by complex neuronal circuits in the frontal lobe which has many neuronal connections to other cortical areas or the basal ganglia.

Summary The effects of spinal cord compression on conduction of compound action potentials (CAPs) in the dorsal column fibers at various stimulus frequencies were analysed in pentobarbital anesthetised cats. The L 6 posterior root was given 1 to 500 Hz stimuli, and the CAPs were recorded from the L 2 posterior column. The 100th CAP was recorded at 100, 200 and 500 Hz. The L 4 cord segment was compressed by stepped increments (0.5 mm/5 min.) with a plastic plate (4 × 10 mm) until the responses at 1 Hz disappeared, then the compression was released. Before compression, each CAP at all frequencies showed almost uniform amplitudes and latencies. During compression, the CAPs were not altered significantly at any frequency until the spinal cord was compressed 2.5 – 4.0 mm. Further compression produced a progressive decrease in amplitude and increase in latency of the CAPs. At 500 Hz, however, the conduction block (amplitude loss, latency increase) was much severer than that produced at lower frequencies. After release of compression, the CAPs at all frequencies recovered progressively, and became almost stable after about 1 hour. The recovery rate of amplitude was only about 30% compared to the amplitude before compression at 500 Hz, and about 50% at 1 and 200 Hz. However, the recovery rate of amplitude at 100 Hz (65%) exceeded those at 1 and 200 Hz. To observe the difference in recovery rates among them, all the trains of CAPs were analyzed. Before compression, the amplitudes were almost uniform at stimulus frequencies as high as 500 Hz. After decompression, the amplitude decreased progressively at 500 Hz. At 100 Hz the amplitude increased and reached a steady level after the 5th CAP. At 200 Hz, the amplitude increased transiently and decreased to that of the 1st CAP. In conclusion, the inability of injured fibers to transmit impulses from high frequency stimuli indicates incomplete impairment of spike generation in the dorsal column fibers, which were injured by compression.

The elevation and clearance of extracellular potassium concentration ([K+]e) were studied following graded contusion injury of the rat thoracic spinal cord. Animals were anesthetized, paralyzed, laminectomized at T9-T10, and then artificially ventilated. A 10-g rod was dropped 1.25, 2.5, or 5 cm onto the dorsal thoracic cord with the dura intact. After impact, and incision of the dura-arachnoid and pial membranes, double-barreled, potassium-selective microelectrodes were inserted midway between the midline and lateral edge of the cord. For all three injury levels, the elevation of [K+]ewas greatest within the first 1000 μm from the dorsal surface. In 50 g-cm injuries, increasing [K+]ewas sometimes observed between 250 and 1000 μm; however such gradients were not typically observed in 25 and 12.5 g-cm injuries. Measured at 3-7 min after injury, the mean peak elevations of [K+]ewere significantly different, measuring 13 ± 2.4, 27 ± 5.5, and 44 ± 4.2 mMfollowing 12.5, 25, and 50 g-cm contusions, respectively. The exponential halftimes of [K+]eclearance averaged 5.8 ± 1.0, 9.2 ± 1.8, and 17 ± 5.7 min for the same respective injury levels. These results indicate that elevation of [K+]efollowing traumatic injury to the spinal cord is a graded phenomenon, dependent on the energy of impact. This finding is consistent with a mechanism in which simple mechanical injury of cell membranes is the proximate cause of potassium release.