6 results on '"Rizzi, Roberto"'
Search Results
2. Spontaneous Calcium Oscillations Regulate Human Cardiac Progenitor Cell Growth.
- Author
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Ferreira-Martins, João, Rondon-Clavo, Carlos, Tugal, Derin, Korn, Justin A., Rizzi, Roberto, Padin-Iruegas, Maria Elena, Ottolenghi, Sergio, De Angelis, Antonella, Urbanek, Konrad, Ide-Iwata, Noriko, D'Amario, Domenico, Hosoda, Toni, Leri, Annarosa, Kajstura, Jan, Anversa, Piero, and Rota, Marcello
- Subjects
INTRACELLULAR calcium ,HEART cells ,CELL communication ,GROWTH factors ,CALCIUM - Abstract
The article focuses on a research that aims to identify the pathways that regulate intracellular Ca
2+ in human cardiac progenitor cells (hCPCs) and to evaluate whether Ca2+ are triggered by hCPCs and cardiomyocytes' interaction. It states that Ca2+ indicator and Fluo-3 and 2-photon microscopy were used to measure cytosolic Ca2+ levels. Results show that hCPCs show spontaneous elevations in intracellular Ca2+ .- Published
- 2009
- Full Text
- View/download PDF
3. A collagen membrane-based engineered heart tissue improves cardiac function in ischemic rat hearts
- Author
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Latronico, Michael V.G., Tampieri, Anna, Condorelli, Gianluigi, Levialdi Ghiron, Jung Hee, Esposito, Giovanni, Schiattarella, Gabriele G., Chiariello, Giovanni A., Sandri, Monica, Rizzi, Roberto, and Pironti, Gianluigi
- Abstract
In the relatively new field of cardiac tissue engineering, different biomaterials, methods and techniques have been tested for cardiac repair, but we are still far from the achievement of a valid model that can be tested for therapeutic goals. In this study, the authors examined the efficacy of newly preformed membranes based on collagen type I for the transplantation of cardiac cells. The membrane prototype, cross-linked with 1,4-butanediol diglycidyl ether (BDDGE) and fibronectin-enriched, gave rise to spontaneously beating heart cell constructs, 5–9 days after seeding with neonatal rat cardiac cells. This membrane was grafted, with and without beating cardiac cells, onto the infarcted area of rat models of heart failure. Seriate echocardiography, performed on rats before transplantation and at 4 and 8 weeks after transplantation, demonstrated that rats treated with collagen membranes previously seeded with beating cells showed an improvement in cardiac function after 8 weeks. These results suggest that this new type of collagen membrane can be used as vector for the transplantation of beating heart cells for the regeneration of the injured myocardium and hence represents an important potential tool for cardiac tissue repair technologies.In the relatively young field of cardiac tissue engineering, different biomaterials, methods and techniques have been tested for cardiac repair, but we are still far from the achievement of a valid model that can be tested for therapeutic goals. In this study we examined the efficacy of new preformed membranes based on collagen type I for the transplantation of cardiac cells. The membrane prototype, cross-linked with 1,4-butanediol diglycidyl ether (BDDGE) and fibronectin-enriched, gave rise to spontaneously beating heart cell constructs 5–9 days after seeding with neonatal rat cardiac cells. This membrane was grafted, with and without beating cardiac cells, onto the infarcted area of rat models of heart failure. Seriate echocardiography, performed on rats before transplantation and at 4 and 8 weeks after transplantation, demonstrated that rats treated with collagen membranes previously seeded with beating cells showed an improvement in cardiac function after 8 weeks. These results suggest that this new type of collagen membrane can be used as vector for the transplantation of beating heart cells for the regeneration of the injured myocardium and hence represents an important potential tool for cardiac tissue repair technologies.
- Published
- 2013
- Full Text
- View/download PDF
4. A scalable, clinically severe pig model for Duchenne muscular dystrophy
- Author
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Stirm, Michael, Fonteyne, Lina Marie, Shashikadze, Bachuki, Lindner, Magdalena, Chirivi, Maila, Lange, Andreas, Kaufhold, Clara, Mayer, Christian, Medugorac, Ivica, Kessler, Barbara, Kurome, Mayuko, Zakhartchenko, Valeri, Hinrichs, Arne, Kemter, Elisabeth, Krause, Sabine, Wanke, Rüdiger, Arnold, Georg J., Wess, Gerhard, Nagashima, Hiroshi, Hrabĕ de Angelis, Martin, Flenkenthaler, Florian, Kobelke, Levin Arne, Bearzi, Claudia, Rizzi, Roberto, Bähr, Andrea, Reese, Sven, Matiasek, Kaspar, Walter, Maggie C., Kupatt, Christian, Ziegler, Sibylle, Bartenstein, Peter, Fröhlich, Thomas, Klymiuk, Nikolai, Blutke, Andreas, and Wolf, Eckhard
- Abstract
Large-animal models for Duchenne muscular dystrophy (DMD) are crucial for the evaluation of diagnostic procedures and treatment strategies. Pigs cloned from male cells lacking DMD exon 52 (DMDΔ52) exhibit molecular, clinical and pathological hallmarks of DMD, but die before sexual maturity and cannot be propagated by breeding. Therefore, we generated female DMD+/− carriers. A single founder animal had 11 litters with 29 DMDY/−, 34 DMD+/− as well as 36 male and 29 female wild-type offspring. Breeding with F1 and F2 DMD+/− carriers resulted in an additional 114 DMDY/− piglets. With intensive neonatal management, the majority survived for 3-4 months, providing statistically relevant cohorts for experimental studies. Pathological investigations and proteome studies of skeletal muscles and myocardium confirmed the resemblance to human disease mechanisms. Importantly, DMDY/− pigs displayed progressive myocardial fibrosis and increased expression of connexin-43, associated with significantly reduced left ventricular ejection fraction, at 3 months. Furthermore, behavioral tests provided evidence for impaired cognitive ability. Our breeding cohort of DMDΔ52 pigs and standardized tissue repositories provide important resources for studying DMD disease mechanisms and for testing novel treatment strategies.
- Published
- 2021
- Full Text
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5. The Effect of the Eradication of Helicobacter pyloriInfection on Hemorrhage Because of Duodenal Ulcer
- Author
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Pellicano, Rinaldo, Peyre, Sergio, Leone, Nicola, Repici, Alessandro, De Angelis, Claudio, Rizzi, Roberto, Rizzetto, Mario, and Ponzetto, Antonio
- Abstract
The cost of a recurrently bleeding duodenal ulcer (DU) is very high, both from a human and an economic point-of-view. Helicobacter pyloriinfection plays an important role in the pathogenesis of DU disease and its complications, such as bleeding. Cure of H. pyloriinfection is recommended in patients with DU and its complications, although in the latter case, the most efficient management is not yet a defined issue. In particular, acid secretion inhibitors may not contribute to long-term cure. Our aims were to ascertain whether the recurrence of bleeding because of DU could be prevented by H. pylorieradication and whether long-term inhibition of gastric acid output is needed to prevent recurrence. Eighty-four patients (65 men; mean age, 55.1 years), who had bled because of recurrent DU, were followed after the cure of H. pyloriinfection. None of the patients were on therapy with nonsteroidal antiinflammatory drugs. Successful cure of H. pyloriwas determined by gastroscopy, histology, and serology performed at 3, 6, 12, 24, and 48 months after the eradication treatment. A 13C urea breath test was performed when the results of serology were unclear and also at recurrence of DU or bleeding. After the antibiotic treatment, 46 patients stopped all medications, whereas 38 continued long-term therapy with histamine type 2 receptor antagonists. During a mean follow-up period of 47.2 months (range, 37–65 months), recurrence of DU at endoscopy was observed in three patients in each group (p0.56), but none bled again. We conclude that H. pylorieradication prevents DU recurrence and rebleeding, that reinfection rate by H. pyloriafter cure was nil at 4 years, and that long-term inhibition of acid secretion may not improve outcome after cure of H. pylori, even in patients whose DU was complicated by hemorrhage.
- Published
- 2001
6. Study on Hp infection in adults with ulcer disease by detection of Hp antigen in stools before and after eradication
- Author
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Peyre, Sergio, Rizzi, Roberto, Predebon, Stefania, Bongera, Mauro, and Ferrini, Arturo
- Published
- 2001
- Full Text
- View/download PDF
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