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Experimental studies have been made to find out Cyanobacterias’ biophotonical response in gaseous-fuelation and carbon dioxide fixation during photo-anaerobic digestion. A new horizontal type photo-bioreactor has been designed by using environment hazard plastic bottles and it works ideally for anoxygenic cyanobacterial growth. Through ‘V3-metagenomics’ of 16S rRNA gene sequencing by paired-end Illumina MiSeq and downstream analysis by QIIME program, we have identified anaerobic cyanobacteria, represent the orders YS2 and Streptophyta. OTUs have been identified by aligning against Greengenes and Silva databases, separately. The flame temperature of the fuel gas is 860°C and the percent-content of carbon dioxide (CO2) is 17.6%.

Experimental studies have been carried out to find the combined effect of visible light and magnesium (Mg0)-metal powder on anaerobic photosynthetic bacteria present in slurry of cowdung and various kinds of organic wastes. The chemical combination of all the above factors helps in producing high quality fuel gas under anaerobic condition. In the fluidized bed reaction, bacteria have been allowed to undergo biophysical and biochemical reactions in the presence of Mg0-metal powder under the continuous flow of photons. The source of photons in the biological media is an artificial light (100 W tungsten bulbs) used at 38 °C. Mg0-metal powder has been added to the slurry to catalyze the biochemical process in presence of light energy. The population rate of bacteria and composition of fuel gas have been estimated under the action of visible photons in presence and absence of induced Mg0-atoms. The fuel gas composition has been measured by Gas-Chromatography and its calorific value measured by flame temperature. Metagenome analysis of consensus ‘V3’-region of 16S rRNA gene amplicons and entire downstream analysis have shown increased bacterial population of Clostridia, Bacteroidiaand γ-proteobacteriain presence of Mg0-atom. Mg0-atom has been observed to activate hydG, mgtEand corAgenes of the bacterial community.