Search

Your search keyword '"Ramsey Y"' showing total 2 results
Start Over Author "Ramsey Y" Database Supplemental Index
2 results on '"Ramsey Y"'

2. From Long Range Mapping to Sequence-Ready Contigs on Human Chromosome 6

Author

Mungall, A. J., Humphray, S. J., Ranby, S. A., Edwards, C. A., Heathcott, R. W., Clee, C. M., Holloway, E., Peck, A. I., Harrison, P., Green, L. D., Butler, A. P., Langford, C. F., Gwilliam, R., Huckle, E. J., Baron, L., Smith, A., Leversha, M. A., Ramsey, Y. H., Clegg, S. M., Rice, C. M., Maslen, G. L., Hunt, S. E., Scott, C. E., Soderlund, C. A., Theaker, A. J., Carter, N. P., Ross, M. T., Deloukas, P., Bentley, D. R., and Dunham, I.

Abstract

Our aim is to construct physical clone maps covering those regions of chromosome 6 that are not currently extensively mapped, and use these to determine the DNA sequence of the whole chromosome. The strategy we are following involves establishing a high density framework map of the order of 15 markers per Megabase using radiation hybrid (RH) mapping. The markers are then used to identify large-insert genomic bacterial clones covering the chromosome, which are assembled into sequence-ready contigs by restriction enzyme fingerprinting and sequence tagged site (STS) content analysis. Contig gap closure is performed by walking experiments using STSs developed from the end sequences of the clone inserts.

Published
1997
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results