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Binge drinking is a significant problem in adolescent populations, and because of the reciprocal interactions between ethanol (EtOH) consumption and the endocannabinoid (eCB) system, we sought to determine if adolescent EtOH intake altered the localization and function of the cannabinoid 1 (CB1) receptors in the adult brain. Adolescent mice were exposed to a 4-day-per week drinking in the dark (DID) procedure for a total of 4 weeks and then tested after a 2-week withdrawal period. Field excitatory postsynaptic potentials (fEPSPs), evoked by medial perforant path (MPP) stimulation in the dentate gyrus molecular layer (DGML), were significantly smaller. Furthermore, unlike control animals, CB1receptor activation did not depress fEPSPs in the EtOH-exposed animals. We also examined a form of excitatory long-term depression that is dependent on CB1receptors (eCB-eLTD) and found that it was completely lacking in the animals that consumed EtOH during adolescence. Histological analyses indicated that adolescent EtOH intake significantly reduced the CB1receptor distribution and proportion of immunopositive excitatory synaptic terminals in the medial DGML. Furthermore, there was decreased binding of [35S]guanosine-5*-O-(3-thiotriphosphate) ([35S] GTPγS) and the guanine nucleotide-binding (G) protein Gαi2 subunit in the EtOH-exposed animals. Associated with this, there was a significant increase in monoacylglycerol lipase (MAGL) mRNA and protein in the hippocampus of EtOH-exposed animals. Conversely, deficits in eCB-eLTD and recognition memory could be rescued by inhibiting MAGL with JZL184. These findings indicate that repeated exposure to EtOH during adolescence leads to long-term deficits in CB1receptor expression, eCB-eLTD, and reduced recognition memory, but that these functional deficits can be restored by treatments that increase endogenous 2-arachidonoylglycerol.

The environment provides many necessary services for a plethora of human activities related to that are being acknowledged as our ecosystems degrade; therefore, ecosystem-based solutions are becoming increasingly more important. Hydrologic restorations (HRs) apply this type of ecosystem approach; there are multiple examples around the Mediterranean area executed since the beginning of the twentieth century. One good example of these restoration-type solutions can be seen in the badlands of Saldaña (Palencia, Spain). This case has been particularly successful in stopping gully erosion from badlands. Despite its importance for the well-being of the inhabitants of Saldaña and surroundings, the economic effects of these services which were put into place by this HR have never been assessed. In the present study, four different and compatible ecosystem restored services have been valued in monetary terms. For their aggregation, an innovative approach has been taken using the analytic hierarchy process methodology, so the weight of each ecosystem service (ES) can be determined. The economic flow of these ESs has been calculated in terms of annual income and updated values, from which natural capital increase in the population of Saldaña can be assessed. After analysing the results, it can be concluded that the village has earned more than seven million euros, which, in turn, generates important benefits to its inhabitants.

Mucormycosis of the gastrointestinal tract is a rare and fatal infection of neonates and children. This report describes a case of disseminated mucormycosis originating in the gastrointestinal tract of a premature infant of 26 weeks gestational age. The antemortem impression was that of necrotizing enterocolitis, diagnosed at 15 days of age, followed by death at 22 days. However, autopsy revealed extensive colonization and invasion of the small intestine and colon by mucormycosis, with blood and peritoneal fluid cultures positive for Mucorales species but negative for bacteria. There was associated hematogenous and adjacent spread of organisms to other viscera.