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Over the past few years, different bioactive chemical antibiotics have been widely abused, but they have received little attention and antibiotics are indeed potential environmental pollutants. Such antibiotics include not only prescription drugs and biological products, but also they contain diagnostic agents, foods, spices, sunscreens, and many others. These compounds and their active metabolites can be continuously transported to the aquatic environment. In this study, we tried to synthesize the origin of antibiotic environment, study the changes of chlorophyll content and soluble protein content under different concentrations of sulfanilamide treated with indoor culture experiments. The results showed that different concentrations of sulfonamides algae growth and chlorophyll synthesis effect of different effects. When the concentration of antibiotics exceeds a certain range, the toxicity mechanism is enhanced; the inhibition of algae growth and chlorophyll synthesis is strengthened. This study explored the potential adverse effects of SMM on aquatic organisms and found that Chlorella has a higher sensitivity to SMM. Therefore, the residual SMM in water should be carefully evaluated and applied to reduce the impact on the ecological environment after farmed animals. [ABSTRACT FROM AUTHOR]