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Abstract: This report evaluates the natural history and clinicopathologic variables of patients with pseudomyxoma peritonei (PMP), and reviews the authors' experience with different treatments in the current literature. PMP results from implantation of malignant tumors or peritoneal irritation caused from ruptured benign cysts. This entity is traditionally characterized by the accumulation of mucinous ascites and a relatively long survival period. Diffuse peritoneal spread occurs in most patients with PMP, and distant metastasis is infrequent. Disease progression is difficult to predict because of the spectrum of underlying pathologic processes with varying malignant potential. Debulking surgery, radiation therapy and chemotherapy have all been advocated for optimal patient management.

Here, we present a protocol to image a fluorescent-labeled intraflagellar transport (IFT) component in Caenorhabditis eleganswith fluorescence microscopy, including steps of sample preparations, in vivolive-cell imaging, and post microscopy analysis with kymographs. This protocol breaks down all processes into three categories: 1) pre-imaging preparations, 2) preparations for the time of image acquisition, and 3) post-imaging analyses. The protocol can be applied to determine the speed and frequency of moving particles.