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Piper aduncumL., Piperaceae, has been used to treat mainly inflammatory diseases and has shown several biological activities such as insecticidal and larvicidal. The aim of this study was to analyze the chemical composition of essential oil of P. aduncumand its efficacy to egg-hatching inhibition of Haemonchus contortusfrom sheep. The essential oil was obtained from leaves and analysed by gas chromatography coupled to flame ionization detector and gas chromatography coupled to mass spectrometry. It was possible to characterize 22 different substances, among them monoterpenes(80.6%) and sesquiterpenes(13.9%). The major compound was identified as 1,8-cineole (55.8%). Eggs of the nematode were exposed to four concentrations of the essential oil. Levamisole phosphate was used as positive control. The essential oil showed to be effective in inhibiting H. contortushatchability and the LC90was calculated as 8.9mg.ml-1. These results can point out the P. aduncumessential oil and its chemical components as potential alternative to control of H. contortus.

A simple method for the resolution and subsequent quantitative determination of liriodenine and moupinamide in Mollinedia species was developed using reversed-phase HPLC based on an octadecyl silane-packed column eluted with gradients of methanol and water. Copyright © 2001 John Wiley & Sons, Ltd.

A simple method for the resolution and subsequent quantitative determination of liriodenine and moupinamide in Mollinediaspecies was developed using reversed‐phase HPLC based on an octadecyl silane‐packed column eluted with gradients of methanol and water. Copyright © 2001 John Wiley & Sons, Ltd.