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The article discusses a study conducted to examine the role of chloroplast protein mutant, Brassinazole (Brz)-insensitive-pale green3-lD (bpg3-lD) in plant organs development and chloroplast development. Topics include photosynthetic genes, DNA extraction and chlorophyll measurement. The results indicate that BPG3 plays an important role in regulating photosynthesis in the chloroplast under brassinosteroid signaling (BR) signaling.

Mutant populations are indispensable tools for investigating plant gene functions. Gain-of-function technology is one of the approaches used for the systematic production of mutant resources and activation tagging is a well-established method to generate gain-of-function mutants in plants. As an alternative approach for the systematic generation of a gain-of-function mutant population, we developed the Full-length cDNA OvereXpressor (FOX)-hunting system in which full-length cDIMAs (fl-cDNAs) are overexpressed in plants to quickly identify candidate genes.The FOX-hunting system was used for high-throughput analysis of rice (Oryza sativa) genes heterologously expressed in Arabidopsis thaliana (rice FOX Arabidopsis lines). A large screening to identify and characterize rice genes with rice FOX Arabidopsis lines revealed that one of the isolated genes, BROAD-SPECTRUM RESISTANCE 1 (BSR1) conferred multiple or broad-spectrum disease resistance in both a dicotyledonous and monocotyledonous plant. We found that expression of rice fl-cDNAs without a homolog in Arabidopsis affected morphological traits. In addition, overexpression of homologous genes of rice and Arabidopsis led to a similar phenotype.Thus, we conclude that the FOX-hunting system is an excellent heterologous system and offers a new tool with which to explore gene function in rice. [ABSTRACT FROM AUTHOR]