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A novel vacuum zone melting process for preparing high-purity Al was proposed. The axial segregation of impurities was investigated by determining the equilibrium distribution coefficient. Theoretical and experimental results showed that impurities (Cu, Si, and Fe) with an equilibrium distribution coefficient k0<1 accumulated in the tail of the Al ingot, while Ti (k0>1) accumulated at the head end of the ingot. When the molten area moving at 1.5 mm/min and zone melting was performed 15 times, the removal rate of the impurities and the purity of Al were the highest. In the middle of the Al ingot, i.e. 140 mm away from the head end of the sample, the removal rates of Cu, Fe, and Si were higher than 90%, that of Ti was higher than 75%, and the Al content was higher than 99.999%. The purity of the Al samples met the requirements of the commercial 99.999% (5N) high-purity Al standard.

Lead halide perovskites have been explored as a new kind of promising X-ray with wide applications in radiation-associated fields, but low light yield and serious toxicity extremely restrict further applications. To address these issues, we herein demonstrated one new zero-dimensional (0D) organic–inorganic hybrid cuprous halide of [BAPMA]Cu2Br5(BAPMA = N,N-Bis(3-aminopropyl) methylamine) containing discrete [Cu4Br10]6–tetramers as excellent lead-free scintillators. Upon UV light excitation, [BAPMA]Cu2Br5displays highly efficient broadband yellowish-green light emission with one dominant peak at 526 nm, a large Stokes shift of 244 nm, and a high photoluminescent quantum yield of 53.40%. Significantly, this broadband light emission can also be excited by higher-energy X-ray as radioluminescence with a high scintillation light yield of 43,744 photons/MeV. The detection limit of 0.074 μGyair/s is also far less than the required value for regular medical diagnostics of 5.5 μGyair/s. The solution-assembled hybrid structure facilely enables the [BAPMA]Cu2Br5-based scintillation screen to display high-performance X-ray imaging with a spatial resolution of 15.79 lp/mm showcasing potential application in X-ray radiography. In brief, combined merits of low toxicity and cost, negligible self-absorption, a low detection limit, considerable light yield, and spatial resolution highlight the excellent scintillation performance of 0D hybrid cuprous halide.

X-ray scintillators as functional energy materials possess the powerful ability to convert high-energy radiation into visible light with wide applications in various nuclear radiation fields. In this regard, three-dimensional (3D) lead perovskite nanocrystal-based X-ray scintillators have attracted extensive attention, but their low light yield and serious toxicity extremely restrict their further applications. To address these issues, a family of 0D hybrid cuprous halides of A2Cu4X6(A = PTPP, TPA; X = Br, I) based on discrete [Cu4X6]2−nanoclusters were demonstrated as highly desirable lead-free scintillators. Upon excitation of both ultraviolet and blue light, these halide nanoclusters displayed that self-trapped excitons induced broadband light emissions from green to red with near-unity photoluminescent quantum yield (PLQY, 93.1%) andlarge Stokes shifts (>1.3 eV). Significantly, the high PLQY, negligible self-absorption, and strong X-ray attenuation from [Cu4X6]2−nanoclusters endowed them with extraordinary radioluminescence properties. The linear radioluminescence intensity response to a wide range of X-ray dose rates gave an acceptable detection limit of 0.7563 µGyairs−1, which was lower than the required value for regular medical diagnostics (5.5 µGyairs−1). X-ray imaging demonstrated an ultrahigh spatial resolution of 14.83 lp mm−1and negligible afterglow (1.3 ms), showcasing potential applications in X-ray radiography. Overall, the combined superiorities of nontoxicity, high light yield, excellent stability, and good radiation hardness make cuprous halide nanoclusters excellent scintillators.

Multimodal imaging used in the evaluation of severity of acute multifocal hemorrhagic retinal vasculitis and follow up for treatment response. Timely aggressive immunosuppressive therapy is beneficial for treating acute multifocal hemorrhagic retinal vasculitis.

Introduction:Aged kidney is characterized by mitochondrial dysfunction, cellular senescence, and fibrogenesis. The activation of Wnt/β-catenin signaling plays an important role in the initiation of kidney aging. However, the inhibiting strategies have not been discovered in detail. Here, we compared the therapeutic effects of two β-catenin inhibitors, KYA1797K and ICG-001, to assess their superiority. Methods:Two-month-old male C57BL/6 mice which had undergone unilateral nephrectomy and received D-galactose (D-gal) injection were co-treated with KYA1797K or ICG-001 at 10 mg/kg/day for 4 weeks. Human proximal renal tubular cells were treated with D-gal and KYA1797K/ICG-001 to compare their effects. Results:Compared with ICG-001, which inhibits β-catenin pathway through blocking the binding of β-catenin and cAMP response element-binding protein (CREB)-binding protein (CBP), KYA1797K, a novel small molecule destabilizing β-catenin through activating Axin-GSK3β complex, possesses the superior effects on protecting against kidney aging. In D-gal-treated accelerated aging mice, KYA1797K could greatly inhibit β-catenin pathway, preserve mitochondrial homeostasis, repress cellular senescence, and retard age-related kidney fibrosis. In cultured proximal tubular cells, KYA1797K shows a better effect on inhibiting cellular senescence and could better suppress mitochondrial dysfunction and ameliorate the fibrotic changes, at the same dose as that in ICG-001. Conclusion:These results show that effectively eliminating β-catenin is a necessity to target against age-related kidney injury, suggesting the multiple transcriptional regulation of β-catenin in kidney aging besides T-cell factor/lymphoid enhancer-binding factor family of transcription factors (TCF/LEF-1).

Irritable bowel syndrome (IBS) is characterized by visceral hypersensitivity (VH) associated with abnormal serotonin/5-hydroxytryptamine (5-HT) metabolism and neurotrophin-dependent mucosal neurite outgrowth. The underlying mechanisms of VH remain poorly understood. We investigated the role of 5-HT7receptor in mucosal innervation and intestinal hyperalgesia. A high density of mucosal nerve fibres stained for 5-HT7was observed in colonoscopic biopsy specimens from IBS patients compared with those from healthy controls. Staining of 5-HT3and 5-HT4receptors was observed mainly in colonic epithelia with comparable levels between IBS and controls. Visceromotor responses to colorectal distension were evaluated in two mouse models, one postinfectious with Giardiaand subjected to water avoidance stress (GW) and the other postinflammatory with trinitrobenzene sulfonic acid-induced colitis (PT). Increased VH was associated with higher mucosal density of 5-HT7-expressing nerve fibres and elevated neurotrophin and neurotrophin receptor levels in the GW and PT mice. The increased VH was inhibited by intraperitoneal injection of SB-269970 (a selective 5-HT7antagonist). Peroral multiple doses of CYY1005 (a novel 5-HT7ligand) decreased VH and reduced mucosal density of 5-HT7-expressing nerve fibres in mouse colon. Human neuroblastoma SH-SY5Y cells incubated with bacteria-free mouse colonic supernatant, 5-HT, nerve growth factor, or brain-derived neurotrophic factor exhibited nerve fibre elongation, which was inhibited by 5-HT7antagonists. Gene silencing of HTR7also reduced the nerve fibre length. Activation of 5-HT7upregulated NGFand BDNFgene expression, while stimulation with neurotrophins increased the levels of tryptophan hydroxylase 2 and 5-HT7in neurons. A positive-feedback loop was observed between serotonin and neurotrophin pathways via 5-HT7activation to aggravate fibre elongation, whereby 5-HT3and 5-HT4had no roles. In conclusion, 5-HT7-dependent mucosal neurite outgrowth contributed to VH. A novel 5-HT7antagonist could be used as peroral analgesics for IBS-related pain.

Irritable bowel syndrome (IBS) is characterized by visceral hypersensitivity (VH) associated with abnormal serotonin/5-hydroxytryptamine (5-HT) metabolism and neurotrophin-dependent mucosal neurite outgrowth. The underlying mechanisms of VH remain poorly understood. We investigated the role of 5-HT7receptor in mucosal innervation and intestinal hyperalgesia. A high density of mucosal nerve fibres stained for 5-HT7was observed in colonoscopic biopsy specimens from IBS patients compared with those from healthy controls. Staining of 5-HT3and 5-HT4receptors was observed mainly in colonic epithelia with comparable levels between IBS and controls. Visceromotor responses to colorectal distension were evaluated in two mouse models, one postinfectious with Giardiaand subjected to water avoidance stress (GW) and the other postinflammatory with trinitrobenzene sulfonic acid-induced colitis (PT). Increased VH was associated with higher mucosal density of 5-HT7-expressing nerve fibres and elevated neurotrophin and neurotrophin receptor levels in the GW and PT mice. The increased VH was inhibited by intraperitoneal injection of SB-269970 (a selective 5-HT7antagonist). Peroral multiple doses of CYY1005 (a novel 5-HT7ligand) decreased VH and reduced mucosal density of 5-HT7-expressing nerve fibres in mouse colon. Human neuroblastoma SH-SY5Y cells incubated with bacteria-free mouse colonic supernatant, 5-HT, nerve growth factor, or brain-derived neurotrophic factor exhibited nerve fibre elongation, which was inhibited by 5-HT7antagonists. Gene silencing of HTR7also reduced the nerve fibre length. Activation of 5-HT7upregulated NGFand BDNFgene expression, while stimulation with neurotrophins increased the levels of tryptophan hydroxylase 2 and 5-HT7in neurons. A positive-feedback loop was observed between serotonin and neurotrophin pathways via 5-HT7activation to aggravate fibre elongation, whereby 5-HT3and 5-HT4had no roles. In conclusion, 5-HT7-dependent mucosal neurite outgrowth contributed to VH. A novel 5-HT7antagonist could be used as peroral analgesics for IBS-related pain.

Objective: While liver biopsy is the golden standard for liver-fibrosis diagnosis, it is also invasive and has many limitations. Non-invasive techniques such as Magnetic Resonance Imaging (MRI) need to be further developed for liver fibrosis staging. This study aimed to evaluate the diagnostic accuracy of Gadolinium Ethoxybenzyl Diethylenetriamine Penta-acetic Acid (Gd-EOBDTPA)- enhanced MRI for liver fibrosis through systematic review and meta-analysis. Methods: This study comprehensively searched relevant article in PubMed, Embase, and the Cochrane Library published from 2004 to 2018 to find studies analyzing the diagnostic accuracy of Gd-EOB-DTPA-enhanced MRI for liver fibrosis. Two reviewers independently screened the retrieved articles, extracted the required data from the included studies, and evaluated the methodological quality of the studies. The pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio, and Summary Receiver Operating Characteristics (SROC) curve were assessed. Results: This study finally included 16 studies (n = 1,599) and selected a random-effects model based on the results of the I2 statistic to combine them. The areas under the SROC curve for the detection of F1 or greater, F2 or greater, F3 or greater, or F4 liver fibrosis were 0.8669, 0.8399, 0.8481, and 0.8858, respectively. Conclusion: Gd-EOB-DTPA-enhanced MRI showed a good diagnostic performance for staging liver fibrosis, especially for F4 liver fibrosis.

Ultrafast ultrasound imaging has been demonstrated to be an effective method to evaluate carotid stiffness through carotid pulse-wave velocity (PWV) with high reproducibility, but a lack of reference values has precluded its widespread use in clinical practice. The aims of this study were to establish reference values of PWV for ultrafast ultrasound imaging in a prospective, multicenter, population-based cohort study and to investigate the main determinants of carotid PWV.

Introduction:Acute kidney injury (AKI) is at a high prevalence in hospitalized patients, especially in those receiving chemotherapy. Cisplatin is the most widely used chemotherapy drug; however, with its side effects that include nephrotoxicity, it also exhibits a risk of inducing AKI. Importantly, recent studies have shown that autophagy plays a protective role in cisplatin-induced AKI. However, therapeutic strategies and candidate drugs for inducing activation of autophagy remain limited. Methods:In the present study, we adopted a novel candidate drug from a deep sea-derived Penicilliumstrain, penicilliumin B, to testify its protective role in cisplatin-induced renal tubular cell injury. Results:Penicilliumin B exhibited protection against cisplatin-induced apoptosis in cultured renal tubular epithelial cells and in cisplatin-treated mice. Moreover, penicilliumin B maintained normal mitochondrial morphology and inhibited the production of mitochondrial reactive oxygen species. Further studies demonstrated that penicilliumin B enhanced autophagic flux, promoted the activation of multiple autophagy-related proteins, such as mTOR, Beclin-1, ATG5, PINK1, and LC3B, and induced the degradation of p62. Interestingly, we also found penicilliumin B triggered phosphorylation of adenosine 5‘-monophosphate-activated protein kinase (AMPK), which is an upstream inducer of nearly all autophagy pathways and also an activator of mitochondrial biogenesis. These results suggest that AMPK may represent an activated site of penicilliumin B. Consistently, compound C, an AMPK inhibitor, significantly blocked the protective effects of penicilliumin B on mitochondria and apoptotic inhibition. Conclusion:Taken together, our findings indicate that penicilliumin B represents a novel AMPK activator that may provide protection against renal tubular cell apoptosis through activation of AMPK-induced autophagy and mitochondrial biogenesis.

Layered double hydroxides (LDHs) are typical two-dimensional nanomaterials, which can be easily modified to serve molecular transport channels for improving membrane’s performances. In this study, two types of LDHs, namely citric acid (CA) intercalated LDHs and carbon dots (CDs) intercalated LDHs, have been synthesized and used as nonofillers to modify polyamide (PA) thin-film composite reverse osmosis membranes. The influence of different concentrations of LDHs on the physicochemical properties and morphologies of the RO membranes was investigated. The chemical composition of the membranes was measured by Fourier transform infrared spectroscopy and the morphologies of the modified RO membranes were studied by scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. The obtained membranes exhibited sparser leaf-like structures, resulting in a flux of 33.2 L m-2h-1, approximately 36% improvement compared with the unmodified membrane. Our results showed that LDHs modified PA membranes could effectively improve the permeability of the membrane.

Cyclic olefin copolymer (COC) is an amorphous thermoplastic with desirable dielectric and mechanical characteristics for optical applications. In particular, its low refractive index, overall mechanical strength, and absence of strong absorption features make it a promising substrate material for far-infrared applications, which include frequency-selective surfaces, scattering filters, and windows. The dielectric properties of selected COC formulations are surveyed from $ \approx 10 - 700\,\, {{\rm cm}^{ - 1}} $≈10−700cm^−1 ($ 1000 - 14\,\, \unicode{x00B5} {\rm m} $1000−14µm), and representative usage as a thin film membrane structure in optical filters is presented.

A quantitative water detection method is urgently needed in storage facilities, space exploration, and the chemical industry. Although numerous physical techniques have been widely utilized to determine the water content, they still suffer from many disadvantages such as highly expensive special instruments, complicated analysis processes, etc. Hence, a convenient, rapid, and sensitive water analysis method is highly desirable. Herein, we developed a visual fluorescence sensing technology for water detection based on reversible PL off-on switching of organic–inorganic hybrid zero-dimensional (0D) manganese halides. In this work, a family of hybrid manganese halides were synthesized through a facile solution method, namely, [NH4(18-Crown-6)]2MnBr4, [Ca(18-Crown-6)·3H2O](18-Crown-6)MnBr4, [NH4(dibenzo-18-Crown-6)]2MnBr4, and [Ca(dibenzo-18-Crown-6)·2H2O]MnBr4. Excited by UV light, these highly crystalline manganese halides exhibit strong green light emissions from the d–d electron transition of Mn2+with near-unity photoluminescence quantum yield and submillisecond lifetime. Benefiting from the dynamic and weak ionic bonding interactions, these 0D manganese halides display reversible water-response on/off luminescence switching but fail in any other aprotic solvents. Therefore, these 0D hybrid manganese halides can be explored as ultrafast visual fluorescence probes to detect the trace amount of water in organic solvents with multiple superiorities of rapid response time (< 2 s), ultralow detection limit (9.71 ppm), excellent repeatability, etc. The reversible water-response luminescent on/off switching also provides a binary optical gate with advanced applications in anticounterfeiting and information security, etc.

An efficient and novel nitrating reagent has been developed for ipso-nitration of arylboronic acids. By using inexpensive and commercially available Cu(NO3)2/CF3COOH as nitrating reagent, various nitroarenes are produced in moderate to excellent yields (51-96%). Advantages of this procedure are the operational simplicity and no need of extra catalyst.

Peptide-based T cell vaccines targeting the conserved epitopes of influenza virus can provide cross-protection against distantly related strains, but they are generally not immunogenic. Foreign antigen-specific regulatory T (Treg) cells are induced under subimmunogenic conditions peripherally, although their development and role in vaccine-mediated antiviral immunity is unclear. Here, we demonstrated primary vaccination with peptides alone significantly induced antigen-specific Foxp3+Treg cells, which were further expanded by repeated vaccination with unadjuvanted peptides. Certain adjuvants, including CpG, suppressed the induction and expansion of antigen-specific Treg cells by peptide vaccination. Interestingly, secondary influenza virus infection significantly increased the frequency of preexisting antigen-specific Treg cells, although primary infection barely induced them. Importantly, specific depletion of vaccine-induced antigen-specific Treg cells promoted influenza viral clearance, indicating their inhibitory role in vivo. Immunization with CpG-adjuvanted peptides by the subcutaneous prime–intranasal-boost strategy restricted the recruitment and accumulation of antigen-specific Treg cells in lung, and stimulated robust T cell immunity. Finally, subcutaneous prime–intranasal-boost immunization with CpG-adjuvanted peptides or whole-inactivated influenza vaccines protected mice from heterosubtypic influenza virus infection. In conclusion, antigen-specific Treg cells induced by peptide vaccines attenuate the antiviral immunity against influenza virus infection. CpG-adjuvanted peptide vaccines provide heterosubtypic influenza protection probably by inhibiting Treg development and enhancing T cell immunity.

Peptide-based T cell vaccines targeting the conserved epitopes of influenza virus can provide cross-protection against distantly related strains, but they are generally not immunogenic. Foreign antigen-specific regulatory T (Treg) cells are induced under subimmunogenic conditions peripherally, although their development and role in vaccine-mediated antiviral immunity is unclear. Here, we demonstrated primary vaccination with peptides alone significantly induced antigen-specific Foxp3+Treg cells, which were further expanded by repeated vaccination with unadjuvanted peptides. Certain adjuvants, including CpG, suppressed the induction and expansion of antigen-specific Treg cells by peptide vaccination. Interestingly, secondary influenza virus infection significantly increased the frequency of preexisting antigen-specific Treg cells, although primary infection barely induced them. Importantly, specific depletion of vaccine-induced antigen-specific Treg cells promoted influenza viral clearance, indicating their inhibitory role in vivo. Immunization with CpG-adjuvanted peptides by the subcutaneous prime–intranasal-boost strategy restricted the recruitment and accumulation of antigen-specific Treg cells in lung, and stimulated robust T cell immunity. Finally, subcutaneous prime–intranasal-boost immunization with CpG-adjuvanted peptides or whole-inactivated influenza vaccines protected mice from heterosubtypic influenza virus infection. In conclusion, antigen-specific Treg cells induced by peptide vaccines attenuate the antiviral immunity against influenza virus infection. CpG-adjuvanted peptide vaccines provide heterosubtypic influenza protection probably by inhibiting Treg development and enhancing T cell immunity.

High-lift siphon drainage by 4-mm internal diameter siphon hoses is a real-time, free-power, and long-term approach for slope drainage. The conventional hydraulics formula for pressurized pipe flow is generally used to calculate the single-phase velocity of siphon flow. However, an intensive cavitation phenomenon occurs in the high-lift siphon hose and then a two-phase flow is formed. Research on the velocity of high-lift siphon flow is a prerequisite for the application of siphon drainage with a 4-mm siphon hose. Few investigations of this subject have been carried out. Hence, experiments on the high-lift (8 m≤H0≤10.3 m) siphon drainage in a 4-mm siphon hose were performed. The characteristics of siphon flow under different conditions were observed and test data were obtained. Comparisons between test results and calculated results showed that significant errors were given by the hydraulics formula. It is demonstrated that the effect of gas in a siphon hose should be included in the calculation of flow velocity. The findings can be used to determine the number of siphon hoses and layout of siphon drainage holes, and provide valuable information for geotechnical companies.

Sodium acrylate (NaAA) and acrylamide (AM) were grafted onto poly (vinyl alcohol) (PVA) using potassium persulfate as an initiator, Graft copolymerization namely poly (vinyl alcohol)-g-poly (acrylamide/sodium acrylate) (PVA-g-PAM/SAC). The poly (vinyl alcohol)-g-poly (vinylamine/sodium acrylate) (PVAMC) was prepraed by Hofmann rearrangement.The PVAMC homogeneous membrane was characterized by fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM).The water resistance of the PVAMC membranes is the best when pH of the PVAMC solutions was 4, at that time the numbers of-NH3+ and-COO- groups trended to be equal, so the isoelectric point was pH=4. At 90 °C the pervaporation of PVAMC composite membrane was tested and showed that the separation factor and the permeate flux were about 1001 and 1341 g/(m2·h) for 90wt% ethanol aqueous solution, and they were about 1297 and 1040 g/(m2·h) for 90wt% isopropanol aqueous solution.

Vitamin D has been considered as an immune modulator, and exerted the effect through the vitamin D receptor (VDR). This study investigated the associations of single-nucleotide polymorphisms (SNPs) of VDRwith the outcomes of Hepatitis C virus (HCV) infection. Three SNPs (rs2228570, rs757343 and rs739837) were genotyped by TaqMan assay among Chinese population, including 538 HCV spontaneous clearance subjects, 834 persistent infection subjects and 1030 uninfected subjects. Binary logistic analyses were used to control the effects of confounding factors. The results showed that subjects with the rs757343 A allele and rs739837 A allele had the significantly reduced risk of HCV susceptibility (all PBonferroni<0.05 in dominant/additive model). In the stratified analysis, the protection of rs757343 A allele and rs739837 A allele against HCV infection remained effective in some subgroups. In addition, patients carrying rs739837 CA genotype were less prone to develop persistent infection (PBonferroni=0.033) and such effect still work in several subgroups in the stratified analysis. Furthermore, haplotype analysis indicated that when compared with the most frequent GC haplotype, the haplotype carrying AA (odds ratio (OR)=0.66, 95% confidence interval (CI)=0.56–0.78) and GA (OR=0.64, 95% CI=0.47–0.85) suggested a protective effect. Our findings indicated that the polymorphisms of VDRare associated with the outcomes of HCV infection among Chinese population.

Based on the intermittent and compartmental energy consuming method of residential buildings in Hot Summer and Cold Winter Zone, a two-dimensional heat transfer model of one household flat is built for a transient study of the wall insulation effect on building energy efficiency. With the mode of compartmental energy consuming, the energy consumption of interior walls accounts for nearly 45% of the total amount, thus insulation for interior walls should be taken into more consideration as well as for exterior walls. With the intermittent energy consuming mode, exterior wall external insulation can decrease air-conditioner heating load. However, when cooling in summer nights, the temperature of wall is higher than both indoor and outdoor. External insulation hinders heat in walls from dissipating to outside, and thus there is an “anti-insulation” effect existing here. Interior insulation can effectively decrease both heating and cooling loads of air-conditioner.

According to the climate and energy saving characteristics in the hot summer and cold winter zone, especially in view of requirements for thermal insulation in summer, reflective thermal insulation coatings (RTIc) are gradually applied in building energy efficiency of exterior envelope. A feasible evaluation method for energy saving effect is very important for acceptance of energy efficient building construction. In this paper, thermal resistance has been used as the evaluation index. The energy efficiency of the reflective thermal insulation coatings was investigated. Four hot boxes were applied contrast test. Based on the qualitative and quantitative analysis of electricity consumption within 36 hours and comparative analysis of heating process simulating sunlight environment, thermal resistance of RTIc can be calculated on the basis of the correlation between power saving rate and thermal resistance so that energy saving effect of RTIc can be quantitatively characterized.

The starch-based polyampholyte (SPAM) was synthesized by graft copolymerization of starch with acrylamide (AM) and acrylic acid (AA). The structural and chemical characteristics of the product, as well as its efficiency in slowing the nitrogen release and water evaporation in soil were examined. The slow-release effect of SPAM was better than starch, starch-g-SPAC (Sodium polyacrylate) and starch-g-PVAC (Polyvinyl ammonium chloride) for urea release, particularly when the pH of SPAM solution was equal to 5(approached or equaled to isoelectric point, IEP). These studies showed that the product with good slow-release and being environmentally friendly is biodegradable carrier materials.

In order to selectively separate luteolin from its crude solution, we synthesized luteolin molecular imprinted polymers (LMIP) with high recognition specificity for luteolin, using an imprinting technique. Luteolin was used as template, methanol as solvent, and N,N’-methylenebisacrylamide (MBAA) as the cross-linking. Then prepared LMIP were characterized and evaluated by scanning electron microscope (SEM) and equilibrium absorption experiments. The results showed that the cavities matching with the template molecules in size and structure were present in the LMIP. Adsorption dynamics analysis suggested that, when the adsorption time reached 4 h, the adsorption process had reached balance and the adsorption capacity was at steady state. The selective adsorption amount reached at 35.65 umol/g for the LMIP, while a lower value of 11.68 umol/g for the blank polymer (i.e. nontemplated). Relative to the corresponding blank polymer, LMIP had an excellent recognition to luteolin in methanol solution. Keywords: Molecular imprinting, Molecular recognition, Adsorbent, Luteolin

The cyclic fluctuation of reservoir water level makes soft rock in bank slope under cyclic seepage pressure and will bring certain effects on permeability characteristics of the soft rock. In order to obtain the relationship between the seepage pressure and seepage time, permeability tests were conducted on red sandstone specimens (from Three Gorges Reservoir Area, China) under cyclic seepage pressures by the self-developed equipment with the function to simulate cyclic fluctuation of reservoir water level. Experimental results show that the water infiltration of the sample was not stable in first three cycles, and it changed from 0.444 mL to 0.086mL. After three cycles, the water infiltration fluctuated between 0.060mL and 0.069mL. It can be drawn that the seepage velocity increase with the raise of pressure and it fit a four times polynomial in each cycle. The study indicates that increasing the seepage pressure appropriately can improve the seepage velocity, and greatly shorten the seepage time.