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Raoultella ornithinolyticais an encapsulated Gram-negative aerobic bacillus belonging to the Enterobacteriaceae family. Originally it used to be a member of Klebsiella. However, in the year 2001, R. ornithinolyticawas reclassified as a new genus type from the Klebsiellaspecies along with two others, and this was based on new genetic approaches. Occurrence of Raoultellain human infections is rare, and its successful identification is confusing. We herein report a misidentification of R. ornithinolyticaby the VITEK-2 Compact automatic microbial identification system (VITEK-2 AMS), but matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA analysis could correctly identify it.

Abstract: Objective: To investigate the clinical significance of the serum VEGF as a marker for monitoring the clinical course of tumor patients cured by surgery and radiotherapy. Methods: Enzyme linked immunosobent assay (ELISA) was used to detect serum levels of VEGF in the patients with carcinoma. Results: X-ray irradiation could induce the tumor cells to express and secret VEGF. Patients with elevated values of serum VEGF 60 days after radiotherapy had higher rate of tumor recurrence and metastasis. There was more chance of metastasis in lung cancer patients with higher level of VEGF after surgical resection (12/21). Less post-operation (3 months-4 years) patients without relapse or cancerometastasis showed elevated values of serum VEGF than those with relapse or cancerometastasis. There was negative correlation between the serum Hb and VEGF in the tumor patients (γ=−0.289, P<0.01). In the 28 patients with normal Hb levels at pre-operation, 17 patients with decreased Hb levels had more chance getting higher VEGF after operation than the others (P<0.05). Conclusion: clinical manifestation should be considered in the application of serum VEGF as a tumor marker, a prognostic factor, and a recurrence indicator of tumor. To determine the levels of serum VEGF and Hb, correct the low level of Hb and block the effect of VEGF by special means may be helpful for tumor patients.