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Here, we present a protocol for encapsulating DNA molecules under crowded conditions within cell-sized lipid-coated droplets. We describe steps for preparing a lipid-oil mixture and adding an aqueous solution containing DNA, which, when mixed, forms water-in-oil droplets of radii between ∼5 and 100 μm. We then detail procedures for quantifying the dynamics of DNA molecules in these droplets by analyzing fluorescence microscopy time series using differential dynamic microscopy. This protocol can be utilized to investigate DNA transport within a range of biomimetic and crowded environments.

Surface plasmon polariton scattering from 2D subwavelength silicon wires is investigated using the finite-difference time-domain method. It is shown that coupling an incident surface plasmon polariton to intercavity modes of the particle can dramatically change transmitted fields and plasmon-induced forces. In particular, both transmission and optical forces are highly sensitive to the particle size that is related to the excitation of whispering gallery modes or standing wave modes depending on the particle shape and size. These features might have potential sensing applications.

We demonstrate that a single sub-wavelength nanoaperture in a metallic thin film can be used to achieve dynamic optical trapping and control of a single dielectric nanowire. A nanoaperture can trap a nanowire, control its orientation when illuminated by a linearly polarized incident field, and rotate the nanowire when illuminated by a circularly polarized incident field. Compared to other designs, this approach has the advantage of a low-power driving field entailing low heating and photodamage.