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The carnivorous Yellowtail Snapper Ocyurus chrysurushas the potential for production in aquaculture. However, there is a lack of studies investigating the digestibility of ingredients needed to formulate balanced diets for this species. The in vitro protein digestibility of fish meal (FM), poultry by‐product meal (PBM), poultry meal (PM), protiblend, canola meal (CM), soybean meal, and wheat gluten was evaluated using the pH‐stat technique. The in vivo digestibility was determined by formulating test diets with a 70:30 ratio of the reference diet and the test ingredients. In terms of in vitro digestibility, FM, PBM, and PM exhibited the highest degree of hydrolysis (DH). Conversely, PBM displayed the lowest apparent digestibility coefficient of protein (ADCprotein) in the in vivo digestibility test. Meanwhile, FM, PM, and CM demonstrated higher values of protein digestibility. Beta regression between ADCproteinand the DH and ash content of the ingredients resulted in a high correlation (R2= 0.94). The results of the present study indicate that Yellowtail Snapper efficiently digested and absorbed FM, PM, and CM ingredients, which contain a lower content of ash. Impact statementSeveral fish species are overexploited in the production of fish meal, the primary ingredient in fish feed. Therefore, the comprehensive evaluation of potential ingredient substitutes for fish meal is imperative to foster the sustainable development of aquaculture. Several fish species are overexploited in the production of fish meal, the primary ingredient in fish feed. Therefore, the comprehensive evaluation of potential ingredient substitutes for fish meal is imperative to foster the sustainable development of aquaculture.

Ultra-black coatings have served various roles in space instrumentation and hardware. The Ebonol C coating has been used for various missions requiring low reflectivity on aluminum substrates that are electrically conducting. This coating is produced through the formation of a cupric oxide structure on an intermediate layer of copper applied to the aluminum. For many years, this commercial formulation has been used ubiquitously for space instruments. The coating relies upon details of the copper base layer that have been trade secret at various finishing companies, and the availability of the Ebonol C formulated chemistry from the chemical supplier. In recent years, this product has been discontinued at various times, leaving vendors to either rely on expired stock or to formulate their own equivalents. Here, we detail the processing methods, chemistries, and resultant coating structures that produce reflectance performance equivalent to baseline historical reflectance data. This process detail will ensure equivalent performance for such coatings for future missions and link the performance to past and currently deployed instruments.

The number of Electric Vehicles (EVs) on the roads is expected to dramatically rise within the next few years. This is poised to substantially increase the total electricity demand due to EV charging. The key question is whether today’s distributions systems can handle this increased charging demand. Our central hypothesis is that coordinated charging is a must to enable wide scale deployment of EV chargers. Coordinated charging refers to scheduling, and possibly optimizing, the charging action of EVs so that charging is more focused during grid off-peak hours. Due to the importance of charging incentives, the objective of this paper is to take a step back and obtain real data that help gauge EV drivers’ level of acceptance to charging scheduling incentives. Using New York City as a living lab, a case study was carried out to analyze the effectiveness of those incentives. The results of a survey, taken by 119 New Yorkers, shed light on people’s response to charging incentives. For instance, 85% of the survey respondents chose to travel longer for a cheaper EVSE instead of heading to a near EVSE at a higher cost.

El síndrome hemolítico urémico atípico se caracteriza por anemia hemolítica, trombocitopenia y lesión renal aguda; ocasionado por una alteración en la regulación del sistema del complemento; es parte de las microangiopatías trombóticas (MAT) con un daño vascular a nivel histológico de los capilares. En este documento se reportan tres casos de pacientes de sexo femenino, entre los 25 y 55 años, con distintas manifestaciones clínicas iniciales, pero con alteraciones a nivel hematológico y renal que caracterizan al síndrome hemolítico urémico. Se evidenció trombocitopenia, anemia y alteración renal en todas las pacientes, aunque en grado variable. Se discute sobre las manifestaciones clínicas, las diferencias con la púrpura trombocitopénica trombótica, principal diagnóstico diferencial, haciendo énfasis en la importancia de tener un alto índice de sospecha clínica para el diagnóstico oportuno y el tratamiento.

estudios recientes han reportado fenómenos trombóticos o coagulopatía en pacientes con COVID-19. Hay posiciones divergentes en cuanto a la prevención, el diagnóstico y el tratamiento de estos fenómenos, y la práctica clínica actual está basada únicamente en deducciones por extensión a partir de estudios retrospectivos, series de casos, estudios observacionales y guías internacionales desarrolladas previas a la pandemia.

ABSTRACTPrevious research on remediation has examined course placement practices, but little is known about first responders and how their beliefs about proper course placements help maintain patterns in course access. This study examined how taken-for-granted racial beliefs were used as legitimate knowledge by community college counselors. Haney López’s (2000) race as commonsense theoretical perspective was used to analyze interview data from 34 counselors in 2 southern California community colleges. Data showed that counselors recreated race categories and racial hierarchy, and they did so by reinforcing beliefs about white students as intelligent and deserving higher placements and Latina/o students as comparatively lower in ability and deserving remediation. The results suggest that counselors begin institutionalizing racial sorting immediately as first-time students begin to enroll. The findings highlight the importance of racial mindfulnessin policy, practice, and research.

Presentamos un caso interesante de meningoencefalitis aguda por reactivación del virus de varicela zóster en un paciente inmunocomprometido en ausencia de neuralgia dermatomal ni lesiones cutáneas atribuibles al virus de varicela zóster.

La rotura del septum ventricular como complicación mecánica posterior a un infarto agudo de miocardio (IAM) es poco frecuente a partir del uso de medicamentos fibrinolíticos, pero altamente mortal. Se debe sospechar en pacientes con disnea, dolor torácico, soplo pansistólico en borde esternal izquierdo y choque cardiogénico, confirmándose el defecto, la localización y el tamaño a través de ecocardiografía y doppler color. El manejo quirúrgico sigue siendo la mejor opción terapéutica comparado con el manejo médico. Se presenta el reporte de un caso en un paciente masculino adulto mayor con hipertensión arterial, quien desarrolló un IAM con elevación del segmento ST anterolateral. Se realizó fibrinólisis con alteplasa y posteriormente angiografía coronaria más intervención coronaria percutánea de rescate en las primeras 24horas en la arteria descendente anterior. Al cuarto día del inicio del cuadro se documenta la rotura del septum ventricular que lleva a choque cardiogénico y posterior deceso.

La mediastinitis es una infección potencialmente mortal, y debido a su baja incidencia, se cuenta con poca información epidemiológica. Las infecciones odontogénicas complicadas como la angina de Ludwig son una causa poco frecuente de mediastinitis necrotizante descendente, pero causan 90% de las infecciones necrosantes. Se presenta el caso de un paciente masculino de 34 años, con enfermedad periodontal que desarrolla angina de Ludwig. Progresa a mediastinitis necrotizante descendente y shock séptico, requiriendo manejo quirúrgico de urgencia, estancia en UCI, Ventilación mecánica invasiva y manejo antibiótico de amplio espectro, con múltiples complicaciones asociadas al compromiso mediastinal. Se analizan aspectos clave del caso como epidemiología, fisiopatología, diagnóstico y tratamiento de la mediastinitis necrotizante descendente secundaria a infecciones odontogénicas. La angina de Ludwig con mediastinitis necrotizante descendente es una complicación grave pero prevenible este tipo de infecciones. Su manejo requiere un abordaje multidisciplinario oportuno para reducir la morbimortalidad.

Reportar un caso de implante de cierre de ruptura del seno de Valsalva e implante de válvula aórtica transcateterismo.

AbstractA Bayesian analysis of genetic parameters for quantitative traits was carried out in full-sib families of popcorn through four cycles (C5, C6, C7, and C8) of recurrent selection. The parameters of Bayesian model were estimated using the independence chain algorithm. Grain yield and plant height were found to be moderately heritable ranging from 0.25 to 0.48 and 0.45 to 0.58, respectively. The highest heritability estimates were found for popping expansion volume at C5( = 0.71), and C7( = 0.63). The response of recurrent selection was 30.5 and 32.3% for grain yield and popping expansion volume, respectively. In C8, predicted genetic advances (from the Bayesian prediction of genotypic values) were 4.8, 4.3, and 2.9% for plant height, grain yield, and popping expansion volume, respectively. The significant genetic variability among popcorn families in successive cycles of recurrent selection has sustained continued genetic progress in breeding for the traits of interest.

Un meta-análisis de la relación entre la satisfacción laboral de los empleados y la satisfacción de los clientes se llevó a cabo arrojando una correlación estadísticamente significativa para esta relación. Los resultados sugieren que el contexto de servicio es un moderador de la relación entre la satisfacción en el trabajo y la satisfacción de los clientes. Algunas implicaciones para el futuro se presentan investigación.

La cirugía cardiaca con circulación extracorpórea en pacientes obstétricas representa un capítulo especial, debido a los cambios fisiológicos producidos por el embarazo y factores como el manejo anestésico, la monitorización y la perfusión durante el bypass cardiopulmonar que se producen sobre el binomio madre-feto, organismos en situaciones diferentes y con intereses opuestos. Describimos el manejo anestésico de una paciente de 26,2semanas de embarazo con un tromboembolismo pulmonar y trombo auricular adherido a catéter central con comunicación interauricular.

Cardiac surgery with extracorporeal circulation in obstetric patients is a separate chapter because of the physiological changes brought about by pregnancy and factors such as anesthetic management, monitoring and perfusion during the cardiopulmonary bypass that affects both the mother and the child; both experience different circumstances and have dissimilar interests. The paper discusses the anesthetic management of a patient during her 26.2 weeks of gestation with a pulmonary thromboembolism and atrial intracavitary thrombus attached to the central catheter with atrial septal defect.

The Internet has become a growing channel for consumer purchases. Half of all U.S. consumers made at least one purchase on-line in 2001. However, many consumers report frustration with the lack of support for navigation within many Internet retailers' web sites. Several design patterns have been suggested to overcome these limitations, such as expanded hierarchies and breadcrumbs. This study investigated the effects of these design patterns on users' quantitative performance and subjective preference for ecommerce web sites. Expanded hierarchies, a design pattern that is commonly used by many retail web sites, degraded all of the performance metrics assessed in the study. Users required more time, made more errors, used more clicks, and had lower satisfaction scores for sites designed with expanded hierarchies. The results for breadcrumbs suggest that they may improve performance. The inclusion of breadcrumbs reduced the number of clicks required by users to complete the tasks, but other performance metrics did not reach statistical significance. The results indicate that design patterns that are believed to improve performance a priorimay not yield the results expected.

Design and analysis of nonequilibrium separation process problem are discussed. Even though there have been published articles on the design and analysis of equilibrium separation process problems, these issues have not been addressed in great detail as far as nonequilibrium problems are concerned. Strategies are developed for drawing residue curve maps using mass‐transfer model equations. In addition the multistage nonequilibrium separation process problem is rigorously analyzed and it is shown that, as in the single‐stage case, any multiplicity that occurs is a result of the multiplicity that occurs here in the phase equilibrium calculations at the interface.

15-Lipoxygenase 2 (15-LOX2) is a recently cloned human lipoxygenase that shows tissue-restricted expression in prostate, lung, skin, and cornea. The protein level and enzymatic activity of 15-LOX2 have been shown to be down-regulated in prostate cancers compared with normal and benign prostate tissues. The biological function of 15-LOX2 and the role of loss of 15-LOX2 expression in prostate tumorigenesis, however, remain unknown. We report the cloning and functional characterization of 15-LOX2 and its three splice variants (termed 15-LOX2sv-a, 15-LOX2sv-b, and 15-LOX2sv-c) from primary prostate epithelial cells. Western blotting with multiple primary prostate cell strains and prostate cancer cell lines reveals that the expression of 15-LOX2 is lost in all prostate cancer cell lines, accompanied by decreased enzymatic activity revealed by liquid chromatography/tandem mass spectrometry analyses. Further experiments show that the loss of 15-LOX2 expression results from transcriptional repression caused by mechanism(s) other than promoter hypermethylation or histone deacetylation. Subsequent functional studies indicate the following: 1) the 15-LOX2 product, 15(S)-hydroxyeicosatetraenoic acid, inhibits prostate cancer cell cycle progression; 2) 15-LOX2 expression in primary prostate epithelial cells is inversely correlated with cell cycle; and 3) restoration of 15-LOX2 expression in prostate cancer cells partially inhibits cell cycle progression. Taken together, these results suggest that 15-LOX2 could be a suppressor of prostate cancer development, which functions by restricting cell cycle progression.

The Internet provides a fantastic opportunity for users to obtain information for which they would otherwise have no access. However the existence of billions of web pages presents as much of a challenge as it does an opportunity. Surveys of search engine use have determined that they generally do not support efficient and effective searching. Part of this problem is that little consideration has been given to the nature of the search task in designing the output of most search interfaces. This study investigated alternate output structures for presenting the results of on-line search. Forty participants completed two search tasks using both the standard list output structure and an alternate tabular structure. The results showed that the tabular structure supported a wider variety of search strategies, including some that were not feasible with the standard list. The tabular structure also supported faster parsing and was preferred by users. The study provides evidence that consideration of multiple search strategies would enhance the design of search output structures.

Equations constituting the nonequilibrium rate‐based separation process problem involving binary and ternary mixtures were analyzed. Multiplicity occurring in the nonequilibrium stage resulted from multiplicity taking place in the equilibrium calculations at the interface. This is achieved by demonstrating a one‐to‐one correspondence between the bulk and interface compositions in a nonequilibrium stage. The analysis of the film model for the mass‐transfer problem where the fluxes are obtained using the Maxwell‐Stefan equations showed solution uniqueness for this equation set. It also showed that the imposition of the mass‐transfer equations does nothing to cause additional multiplicities.

An alternative mevalonate‐independent pathway for isoprenoid biosynthesis has been recently discovered in eubacteria (including Escherichia coli) and plant plastids, although it is not fully elucidated yet. In this work, E. colicells were engineered to utilize exogenously provided mevalonate and used to demonstrate by a genetic approach that branching of the endogenous pathway results in separate synthesis of the isoprenoid building units isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). In addition, the IPP isomerase encoded by the idigene was shown to be functional in vivo and to represent the only possibility for interconverting IPP and DMAPP in this bacterium.

Angiogenesis, the formation of new blood vessels from preexisting ones, is a fundamental stage in the metastatic pathway. For the primary tumor, this neovascularization provides nutrients and oxygen as well as a route by which metastatic tumor cells gain access to the circulatory system. Among these metastatic tumor cells, there are subgroups of cells that express an angiogenesis-inducing cells phenotype (AICs) as well as others that do not. Tumor cells not expressing the angiogenesis-inducing cells phenotype (non-AICs) invade new tissues and remain as dormant micrometastases unless they accompany AICs. Thus, either alone or with non-AICs, angiogenesis-inducing cells form rapidly growing, clinically detectable metastases. Much of the current research in this area is concentrated on the vascularization of primary tumors, but the regulation of angiogenesis by extravasating or invading tumor cells has not being extensively studied. We have developed a working model, which demonstrates that human metastatic prostate cancer cells (PC-3) appear to induce human vascular endothelial cells (HUVECs) to translocate across a Matrigel-coated 8 urn membrane. The parameters of this model (i.e. pore size, seeding-cell density, seeding times) were established using highly invasive murine melanoma cells (B16F10) seeded on murine microvascular endothelial cells (CD3). We have further modified our model in order to include a host compartment made of collagen gel, in order to mimic the in vivo site of metastases-induced angiogenesis.

Human embryonic kidney cells (HEK-7) were transfected with the gene for the human α2C receptor (α2C4). Cells were grown in 96-well microtiter plates and cyclic AMP levels were measured by scintillation proximity assay, a modified radioimmunoassay technique. Radioactivity was quantified using a TopCountTM Scintillation detector. Cyclic AMP was increased in a dose-dependent manner by the addition of exogenous forskolin. The forskolin-induced enhancement of cyclic AMP was inhibited dose-dependently by the addition of α2-adrenergic agonists, and this inhibition was blocked by the addition of adrenergic antagonists. The extent of the inhibitory response caused by α2-adrenoceptor agonists was related to the receptor density in clonal cell lines derived from the transfected parental HEK-7 cells. By using cells grown in microtiter format, and employing the technological advantages of scintillation proximity assay and TopCountTM detection, it was possible to simultaneously evaluate the effects of multiple experimental permutations on cellular production of cyclic AMP with minimal disturbance of the cells and minimal and/or automated manipulation of the cyclic AMP formed. This combination of techniques should allow rapid testing of the actions of adrenergic agonists and antagonists on cells transfected with receptors linked to cyclic AMP formation.