Search

Particle-loaded inks in binder jetting have proven to increase the packing density of the powder bed, to improve the sintering properties, and to selectively change the material composition in the part. However, particles also change the rheological properties and thus the penetration behavior of the ink in the powder bed. The penetration behavior influences the reliability of the binder jetting process. To ensure adequate accuracy and sufficient strength of the green part, an effective connection between the layers is crucial. Insufficient penetration leads to a weak bonding of the layers, while excessive penetration reduces the geometrical accuracy. The prediction of the penetration behavior can be beneficial to set the process parameters for new material systems and to control the distribution of particles in the green part. This study presents a modeling approach for the determination of the penetration depth of particle-loaded inks in the binder jetting process on the basis of the sessile drop method. An additional process model was established that allowed the integration of the process parameters of the binder jetting procedure. The validation was performed by measuring the penetration depth of three inks with particle loads of 1 m%, 5 m%, and 10 m% into a spherical aluminum oxide powder. The comparison of the experimentally determined with the predicted penetration depths showed a good agreement for low particle loads. With higher particle loads, the deviations from the experimental data increased due to an accumulation of the particles on the top of the particle-loaded layer.

Type 2 diabetes mellitus (T2DM) is a genetically influenced disease, but few studies have been performed to investigate the genetic basis of T2DM in Vietnamese subjects. Thus, the potential associations of KCNJ11and ABCC8single nucleotide polymorphisms (SNPs) with T2DM were investigated in a Kinh Vietnamese population. A cross-sectional study consisting of 404 subjects including 202 T2DM cases and 202 non-T2DM controls was designed to examine the potential associations of 4 KCNJ11and ABCC8SNPs (rs5219, rs2285676, rs1799859, and rs757110) with T2DM. Genotypes were identified based on restriction fragment length polymorphism and tetra-primer amplification refractory mutation system polymerase chain reaction. After statistically adjusting for age, sex, and BMI, rs5219 was found to be associated with an increased risk of T2DM under 2 inheritance models: codominant (OR = 2.15, 95% confidence intervals [CI] = 1.09–4.22) and recessive (OR = 2.08, 95%CI = 1.09–3.94). On the other hand, rs2285676, rs1799859, and rs757110 were not associated with an increased risk of T2DM. Haplotype analysis elucidated a strong linkage disequilibrium between the 3 SNPs, rs5219, rs2285676, and rs757110. The haplotype rs5219(A)/rs2285676(T)/rs757110(G) was associated with an increased risk of T2DM (OR = 1.42, 95%CI = 1.01–1.99). The results show that rs5219 is a lead candidate SNP associated with an increased risk of developing T2DM in the Kinh Vietnamese population. Further functional characterization is needed to uncover the mechanism underlying the potential genotype-phenotype associations.

Objective: The aim of this study was to investigate risk factors for cutaneous adverse reactions (CARs) in Kinh Vietnamese. Methods: All patients were prospectively recruited in Ho Chi Minh City. Presence of the HLA-B*58:01 allele was determined by real-time PCR-sequence-specific amplification by using the PG5801 Detection Kit (Pharmigene, Taipei). Patients with severe (SCARs) and mild (MCARs) CARs and controls were compared for differences in features prospectively collected, and odds ratios (ORs) with 95% confidence intervals (CIs) were estimated. Results: On comparing 32 patients with SCARs and 395 tolerant controls, we identified eight strong risk factors: increased age (OR 15.1 [95% CI 5.8–40.1], P< 0.0001), female sex (OR 333 [40–43,453], P< 0.0001), allopurinol for asymptomatic hyperuricemia (OR 955 [120–125,847], P< 0.0001), allopurinol starting dose > 150 mg (OR 316 [101–122], P< 0.0001), diuretics intake (OR 304 [35–40,018], P< 0.0001), eGFR < 60 ml/min/1.73 m2(OR 100 [32–353], P< 0.0001), history of allopurinol-induced skin reaction (OR 78 [6–10,808], P= 0.004), and HLA-B*58:01 carriage (OR 147 [45–746], P< 0.0001). HLA-B*58:01 allele frequency in controls was 7.3%. For MCARs (n= 74), risk factors were eGFR < 60 ml/min/1.73 m2(OR 4.9 [1.61–14.6], P= 0.006), history of allopurinol-induced skin reaction (OR 27 [2–3777], P= 0.01), and asymptomatic hyperuricemia (OR 27 [2–3777], P= 0.01). Conclusion: This study confirmed 8 risk factors, including HLA-B*58:01, for SCARs and identified 3 risk factors for MCARs in Kinh Vietnamese. HLA-B*58:01 genotyping could guide the indication for allopurinol in Kinh Vietnamese patients with gout.